Article
Chemistry, Analytical
Mohammad K. D. Manshadi, Amin Mansoorifar, Jung-Chih Chiao, Ali Beskok
Summary: This study introduces inexpensive, rapid, sensitive, and quantifiable impedance-based immunosensors to evaluate the concentration of neutralizing antibodies (NAb) against the coronavirus. The sensor detection mechanism is mainly based on the diffusion of biomolecules onto the electrode surface. The results demonstrate that the sensor is capable of detecting NAb in the range of 45 ng/mL to 185 ng/mL. Due to its fast test results and lower costs, this sensor can be used to assess NAb in people's blood serum before receiving further COVID vaccine doses.
ANALYTICAL CHEMISTRY
(2023)
Article
Physics, Fluids & Plasmas
Amirreza Malekanfard, Zhijian Liu, Hui Zhao, Yongxin Song, Xiangchun Xuan
Summary: Insulator-based dielectrophoresis (iDEP) is a emerging technique for particle manipulation in microfluidic devices, where induced charge electroosmisis (ICEO) and electrothermal flow (ETF) exhibit different strength vortex patterns in low and high electric conductivity fluids, but become marginal in moderate-concentration buffers due to opposing effects of ICEO and ETF.
PHYSICAL REVIEW FLUIDS
(2021)
Review
Biochemical Research Methods
Min Song, Xiaogang Lin, Zhijia Peng, Maoxiao Zhang, Jayne Wu
Summary: Biosensors are crucial in decentralized diagnostics, with affinity biosensors relying on specific binding between biological ligands and target analytes. Integrating affinity biosensors with ACEK enrichment has the potential to enhance sensitivity and speed in detection.
Article
Biochemical Research Methods
Quan Yuan, Jiamei Huang, Jie Jayne Wu, Nazmul Islam
Summary: This research optimizes the microchannel design for ACET micropumps and achieves effective delivery of biofluids, bringing new progress to the field of on-chip biofluid delivery research and applications.
Article
Engineering, Multidisciplinary
Hamdy Abdelhamid, Omar E. Morsy, Ayman El-Shibiny, Reda Abdelbaset
Summary: This article proposes an integrated platform for manipulating and identifying microorganisms, especially foodborne pathogens. By using an electrokinetic-based actuator and capacitive-based sensor, the manipulation and identification of microorganisms can be achieved. Experiments demonstrate that the platform can rapidly identify pathogens without the need for complex and expensive tools and methods.
ALEXANDRIA ENGINEERING JOURNAL
(2022)
Article
Biochemical Research Methods
Amirreza Malekanfard, Zhijian Liu, Le Song, Akshay Kale, Cheng Zhang, Liandong Yu, Yongxin Song, Xiangchun Xuan
Summary: Insulator-based dielectrophoresis (iDEP) utilizes electric field gradients around insulating structures to manipulate particles, with the potential to trap and concentrate nanoparticles in a ratchet-based iDEP microdevice. The electrothermal flow circulations generated from locally amplified Joule heating are found to be more effective with increasing electric field strength, especially for larger particles and in microchannels with symmetric ratchets.
Article
Computer Science, Information Systems
Duje Coko, Ivo Stancic, Lea Dujic Rodic, Dora Cosic
Summary: In this paper, the development of a novel contactless omnidirectional capacitive proximity sensor is presented. The sensor is energy-efficient and has been extensively evaluated for its performance. It can be easily utilized in various applications, such as mobile robotics and human-machine interaction.
Article
Automation & Control Systems
Yang Cao, Jingyan Dong
Summary: This article proposes a resistive self-sensing approach for soft electrothermal actuators to enable closed-loop control without external sensors. The self-sensing of actuator's deformation is achieved by measuring the resistance change of the embedded microfilament heater. A closed-loop controller was designed using the self-sensed deflection signal, with a switching proportional-integral-derivative control algorithm to handle different responses in the heating and cooling stages. The designed control algorithm showed significantly improved response speed, tracking accuracy, and disturbance rejection compared to open-loop operation.
IEEE-ASME TRANSACTIONS ON MECHATRONICS
(2021)
Article
Biochemical Research Methods
Sandra Stanke, Christian Wenger, Frank F. Bier, Ralph Hoelzel
Summary: This paper presents a simple and fast method using alternating current electrokinetic forces to immobilize sub-micrometer objects onto nanoelectrode arrays. The influenza virus is chosen as a model organism due to its medical relevance. One of the outstanding features is the ability to permanently immobilize viral material to the electrodes, eliminating the need for prior chemical surface modification. The accumulation of viral material over time is observed using fluorescence microscopy, and the influences of side effects like electrothermal fluid flow are discussed. By combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly attracted to the electrode edge.
Article
Engineering, Electrical & Electronic
Ehsan Afshari, Mahshid Amirabadi
Summary: The article introduces a modular converter with stackable cells, which does not require electrolytic capacitors for power conversion, enhancing reliability and maintenance. The design also allows for scalability and modularity.
IEEE TRANSACTIONS ON POWER ELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Fraj Echouchene, Thamraa Al-shahrani, Hafedh Belmabrouk
Summary: The study focused on the application of a novel reaction-surface design and electrothermal force in microfluidic immunosensors, demonstrating that the characteristics of the biosensor are enhanced by using a circular ring design of the sensing area. The results also showed that the initial slope related to the association phase is multiplied by a factor of 2 when the voltage is increased from 10 to 15 V, which is valuable for designing new microfluidic biosensors.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Electrical & Electronic
Semih Taniker, Vincenzo Costanza, Paolo Celli, Chiara Daraio
Summary: We propose the realization of capacitive temperature sensors based on the concept of displacement amplification. Our design utilizes high CTE metallic layers and a low CTE dielectric layer to achieve large out-of-plane displacements and capacitive changes as the temperature increases.
IEEE SENSORS JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Jihye Jang, Seung Won Lee, Seokyeong Lee, Chang Eun Lee, Eui Hyuk Kim, Wookyeong Jin, Sejeong Lee, Youngkyung Kim, Jin Woo Oh, Youngdoo Jung, HoYeon Kim, Hyungseok Yong, Jeongok Park, Sangmin Lee, Cheolmin Park
Summary: In this study, a wireless stand-alone interactive display (WiSID) without electrical interconnection was developed. It demonstrated potential applications in wireless dynamic monitoring of medical fluids and smart braille display.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Analytical
Jose Eladio Flores-Mena, Pablo Garcia-Sanchez, Antonio Ramos
Summary: In this study, we investigate the scattering of metal colloids by an insulating circular post under the influence of an alternating current (AC) electric field. We analyze the effects of electrical forces such as induced dipole-repulsion, induced-charge electroosmotic (ICEO) flow, and dielectrophoresis on the particles. The relative influence of these forces is examined in relation to the frequency of the AC field, particle size, and distance to the post. Numerical simulations are performed to model the scattering of metal colloids in a microchannel with an insulating circular post under alternating current electric fields. The simulation results show that the maximum particle deviation occurs when the applied electric field is parallel to the flow direction, and this deviation is more significant at low electric field frequencies where the ICEO interaction dominates over other mechanisms.
Article
Thermodynamics
Min Sung Kang, Jeong Park, Suk Ho Chung, Chun Sang Yoo
Summary: The study investigated the effect of polyethylene (PE) insulator thickness on flame spread over electrical wire with a copper core of high thermal conductivity. Experimental results showed that flame spread rate (FSR) is influenced by the outer wire diameter and categorized into four sub-regimes as the AC electric field frequency increases.
COMBUSTION AND FLAME
(2022)
Article
Chemistry, Analytical
Kailiang Zhang, Yukun Ren, Meiying Zhao, Tianyi Jiang, Likai Hou, Hongyuan Jiang
Summary: The study introduces a method for manipulating microswimmers using thermal buoyancy-capillary convection in multiple microfluidic systems. By adjusting the voltage applied on microheaters, the direction and velocity of granular samples in liquid buffer can be flexibly controlled, which is applicable to various applications such as cargo delivery and sensing.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Jiyu Meng, Tiejun Li, Jingmeng Cheng, Feng Pan, Junwei Li
Summary: In this study, a microfluidic chip based on co-flow step emulsification was proposed for droplet digital PCR (ddPCR), which combines droplet generation, PCR amplification, and fluorescence detection on a single device. The chip offers low cost, easy operation, excellent quantitative capability, and small size, making it a promising tool for DNA molecule quantification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Shanshan Li, Jiaqi Zhang, Yanrong Li, Yang Xu
Summary: In this study, porphyrin-based NCPs with dual fluorescence emission were synthesized via a solvothermal process. These materials could be used for ratiometric fluorescent sensing of pH and copper ion. NCPs@PEI-1 showed a good fluorescence response to pH and could also be used as a sensor for detecting Cu2+.
Article
Engineering, Electrical & Electronic
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Jiyu Meng, Tiejun Li, Feng Pan, Zichao Wang, Junwei Li
Summary: This study presents a reliable microfabrication method for producing tapered glass capillaries using a combination of chemical etching and mechanical polishing. The method offers advantages in terms of size control and surface quality, making it highly promising for the microfabrication of glass capillary devices.
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
(2022)
Article
Chemistry, Analytical
Jiyu Meng, Chengzhuang Yu, Shanshan Li, Chunyang Wei, Shijie Dai, Hui Li, Junwei Li
Summary: In this study, the relationship between microfluids and the glass substrate in microfluidic devices was investigated. With an intelligent structure design and liquid metal, temperature sensing of micro-scale fluids using a millimeter-scale industrial temperature sensor was demonstrated. The proposed heat transfer model and temperature control method showed excellent performance in temperature-sensitive experiments.
Article
Chemistry, Analytical
Chengzhuang Yu, Shanshan Li, Chunyang Wei, Shijie Dai, Xinyi Liang, Junwei Li
Summary: This article introduces the design of a portable and low-cost fluorescence microscope and evaluates its performance. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. By using 3D print components and a webcam, the microscope is assembled at a low cost.
Article
Chemistry, Analytical
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Tiejun Li, Jiyu Meng, Junwei Li
Summary: The presented microfluidic chip provides a possible solution for achieving 3D cell culture and enabling scalable and high-throughput cell culture, with great potential for application in biological fields.
Article
Chemistry, Analytical
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Jiyu Meng, Tiejun Li, Jingmeng Cheng, Junwei Li
Summary: This study proposes a droplet-based multivolume microfluidic device for easy-to-operate and integrated droplet digital PCR (ddPCR), achieving DNA molecule quantification with a wide dynamic range, easy operation, and miniaturization.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Chengzhuang Yu, Shijie Dai, Shanshan Li, Junwei Li, Hezhi Hu, Jiyu Meng, Chunyang Wei, Jie Jayne Wu
Summary: A rapid nucleic acid detection method was developed in this study, using PCR and ICP technology combined with Nafion film for enrichment. The capability of the method was demonstrated through an animal-derived component detection experiment for meat product identification applications, with a detection time of about 35 min.
Article
Chemistry, Analytical
Wei Zhou, Xingyu Li, Wanyun Su, Hongbiao Zheng, Guangxin An, Zhilin Li, Shanshan Li
Summary: This research proposes a method based on a microfluidic chip to obtain a uniform distribution of somatic cells and improve counting accuracy. Experimental results show the significance of this method for the detection and prevention of mastitis.
Article
Biochemical Research Methods
Chengzhuang Yu, Shijie Dai, Ziqi Zhang, Shanshan Li, Jingmeng Cheng, Hezhi Hu, Jayne Wu, Junwei Li
Summary: Meat adulteration detection is achieved using a multiplex digital polymerase chain reaction method and a low-cost device. A polydimethylsiloxane microfluidic device is used to automatically load polymerase chain reaction reagents into microchambers. The method can distinguish DNA templates from different animal species and has a limit of detection of 3 copies/μL. The method has potential for point-of-care testing with portable microscopy equipment.
Article
Chemistry, Analytical
Bowen Yang, Chao Wang, Xinyi Liang, Jinchao Li, Shanshan Li, Jie Jayne Wu, Tanbin Su, Junwei Li
Summary: Cell viability is essential for drug screening. This study proposed a low-cost microfluidic impedance cytometry device with 100-micron wide indium tin oxide (ITO) electrodes to assess cell viability without the cytotoxicity of dye staining.
Article
Engineering, Electrical & Electronic
Shanshan Li, Chao Wang, Bowen Yang, Xinyi Liang, Junwei Li
Summary: Impedance flow cytometry, as a label-free single cell analysis approach, provides valuable differential signals. However, particle coincidence is inevitable for high throughput detection, especially with high cell concentration or pumping rate. In this study, a numerical model and a novel method for particle coincidence were proposed to investigate multiparticle events. By statistically analyzing signal peaks, the threshold for particle events and the particle numbers were determined. Compared to customized thresholds, a data-driven cutoff line fundamentally improves the accuracy of particle recognition. Moreover, the key operational parameters and the best practices to avoid multiparticle coincidence events in real applications are elucidated.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Analytical
Jinchao Li, Jingmeng Cheng, Shanshan Li, Jie Jayne Wu, Junwei Li
Summary: In this research, we developed a chamber-based digital PCR (cdPCR) microfluidic device compatible with fluorescence imaging systems for detecting milk adulteration. The device enables digitalization of PCR reagents in microchambers, followed by DNA amplification through thermocycling. Fluorescence images of the microchambers are then analyzed to accurately quantify the target DNA and differentiate intentional adulteration from accidental contamination. The device was validated by authenticating camel milk samples, demonstrating its effectiveness in real biotechnical applications.
Article
Computer Science, Information Systems
Wei Zhou, Wanyun Su, Xingyu Li, Zhaozong Meng, Shanshan Li
Summary: This paper proposes a feature extraction and classification method based on x-ray images to improve the defect recognition rate of thermal batteries. By combining the Gray Level Co-occurrence Matrix and Local Binary Pattern, the gray texture of the monomer is extracted and analyzed for serial feature fusion. The experimental results show that this method achieves a defect recognition rate of 98.9%.