Article
Environmental Sciences
Yan Zhang, Jing Zhao, Bian -hua Zhou, Er-jie Tian, Wei-shun Tian, Hong-wei Wang
Summary: The study found that a low molybdenum diet led to significant atrophy of the thymus in mice, with decreased thymus weight and organ index. Differential protein analysis and bioinformatics revealed that the proteins affected by low molybdenum were mainly involved in protein metabolism and nucleic acid binding activity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Medicine, Research & Experimental
Jie Zhu, Ling Han, Ruilai Liu, Zhenghua Zhang, Qiong Huang, Xu Fang, Ke Yang, Guiqin Huang, Zhizhong Zheng, Nikhil Yawalkar, Hui Deng, Kexiang Yan
Summary: By analyzing the proteome profile of PBMCs from patients with PsA, PsO, and healthy controls, this study identified 14 potential biomarkers for PsA diagnosis. Additionally, the expression of SIRT2 was significantly higher in PsA patients compared to PsO and healthy controls, and was negatively correlated with the phosphorylation of p38MAPK.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemical Research Methods
Jongmin Woo, Madeline Schoenfeld, Xinguo Sun, Thierry Iraguha, Zhanxiang Zhou, Qibin Zhang
Summary: Using a simplified method, we conducted a proteomics study of intestinal tissue enriched with Paneth cells and identified several proteins associated with Paneth cell characteristics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Fisheries
Lijie Liu, Lidong Lin, Zengling Ma, Guangce Wang, Mingjiang Wu
Summary: High temperature stress destroyed the cell membrane of Sargassum fusiforme, leading to differential expression of proteins mainly involved in glycolysis, nucleoside diphosphate metabolism, and carbohydrate catabolism. Proteomics analyses showed that proteins associated with synthesis, folding, degradation, photosynthesis, energy, and carbohydrate metabolism exhibited differential expression under high temperature stress.
AQUACULTURE RESEARCH
(2021)
Article
Biochemical Research Methods
Yanjuan He, Xupeng Zang, Jingjing Kuang, Huaqiang Yang, Ting Gu, Jie Yang, Zicong Li, Enqin Zheng, Zheng Xu, Gengyuan Cai, Zhenfang Wu, Linjun Hong
Summary: Protein profiling in the uterine luminal fluid during different stages of the estrus cycle and pregnancy in pigs was investigated using iTRAQ proteomics technology. The study revealed significant differences in protein composition during embryo implantation and identified key proteins involved in this process as well as embryonic development.
JOURNAL OF PROTEOMICS
(2022)
Article
Medicine, Research & Experimental
Dingnan Wang, Han Yu, Yunzhou Li, Zongying Xu, Shaohua Shi, Dou Dou, Lili Sun, Zhili Zheng, Xinghua Shi, Xiulan Deng, Xianggen Zhong
Summary: Melatonin has shown significant therapeutic effects against ANIT-induced cholestasis in rats by enhancing antioxidant function and relieving abnormal fatty acid metabolism. iTRAQ proteomics results suggest that melatonin may inhibit ANIT-induced cholestasis through various metabolic pathways such as fatty acid degradation, peroxisome proliferator-activated receptor signaling pathway, and other related pathways.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Clinical Neurology
Dominique F. Leitner, Christopher William, Arline Faustin, Manor Askenazi, Evgeny Kanshin, Matija Snuderl, Declan McGuone, Thomas Wisniewski, Beatrix Ueberheide, Laura Gould, Orrin Devinsky
Summary: This study used mass spectrometry to analyze the differential protein expression in frontal cortex and hippocampus of sudden unexplained death in childhood (SUDC) cases. The results showed activated oxidative phosphorylation and inhibited EIF2 signaling in the frontal cortex, and activated acute phase response in the hippocampus, suggesting potential mechanisms for SUDC. The weighted gene correlation network analysis of clinical history also revealed modules associated with viral pathology, fever, and febrile seizure history.
ACTA NEUROPATHOLOGICA
(2022)
Review
Genetics & Heredity
Xiulan Chen, Yaping Sun, Tingting Zhang, Lian Shu, Peter Roepstorff, Fuquan Yang
Summary: Isobaric labeling technology has become a key tool for comparing protein expression in biological samples, although challenges remain in terms of quantitative accuracy and precision. The different types of isobaric mass tags and advantages and disadvantages of the labeling method are detailed, along with precautions needed at each step of the workflow for reliable quantification results. The broad applications of isobaric labeling technology in biological and clinical studies, with a focus on thermal proteome profiling and proteogenomics, are also discussed.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Agriculture, Dairy & Animal Science
Muhan Zhang, Daoying Wang, Xinglian Xu, Weimin Xu, Guanghong Zhou
Summary: The study identified 301 differentially expressed proteins in duck muscles related to lipid oxidation through iTRAQ analysis and confirmed by PRM. These proteins were found to be involved in lipid binding and metabolism, lipolysis, stress response, oxidative respiratory chain, and redox regulation, potentially impacting lipid oxidation in muscles.
Article
Virology
Yaxin Li, Tao Guo, Xiaokui Wang, Wei Ni, Ruirui Hu, Yuying Cui, Taotao Mi, Shengwei Hu
Summary: The study analyzed the pathogenic mechanisms of Bovine viral diarrhea virus (BVDV) by comparing protein expression levels in infected host cells. Different proteins were found to be differentially expressed at different time points post-infection, mainly involved in metabolic processes, biological regulation, and localization. Various signaling pathways related to BVDV infection and host resistance were enriched at different stages of infection.
Article
Biochemistry & Molecular Biology
Chuanxi Sun, Shuqian Lin, Zhen Li, Huazheng Liu, Yixin Liu, Keke Wang, Tianyi Zhu, Guiming Li, Bin Yin, Renzhong Wan
Summary: Diclofenac sodium (DS), a nonsteroidal anti-inflammatory drug, exhibits toxicity to birds. This study found that DS may induce kidney cell apoptosis, interfere with purine metabolism, and inhibit the expression of OAT2 in chicken kidneys.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Oncology
Agnieszka Fatalska, Natalia Rusetska, Elwira Bakula-Zalewska, Artur Kowalik, Sebastian Zieba, Agnieszka Wroblewska, Kamil Zalewski, Krzysztof Goryca, Dominik Domanski, Magdalena Kowalewska
Summary: Our study aimed to identify protein markers of VSCC tumors for patient stratification based on progression risk. Mass spectrometry-based proteomics revealed immune response as the most over-represented category for differentially expressed proteins (DEPs) in progVSCC vs. d-fVSCC tumors, with pathway profiling indicating bacterial infections linked to aggressive VSCC phenotypes. HMGA2 and PRTN3 were identified as proteins predicting VSCC progression and show promise as markers for patient stratification. Bacterial infections triggering an inflammatory response seem to contribute to cancer progression in VSCC, suggesting that targeted therapies should consider bacterial rather than viral infection status.
Article
Oncology
Keqiang Yan, Bin Bai, Yan Ren, Benliang Cheng, Xia Zhang, Haichao Zhou, Yuting Liang, Lingyun Chen, Jin Zi, Qinghai Yang, Qingchuan Zhao, Siqi Liu
Summary: The tumor microenvironment plays a crucial role in cancer development and metastasis. In colorectal cancer, the protein expression status of the stromal tissues significantly impacts the progression from normal to adenoma and carcinoma. However, the evaluation of protein expression at the stromal level is still limited. This study recruited 22 CRC patients and used laser capture microdissection and mass spectrometry to analyze the proteomic characteristics at different stages of CRC. The results revealed distinctive protein abundance patterns between the stroma and epithelial tissues, indicating the importance of stromal proteins in CRC development. The biochemical features of the tumor stromal proteomes were characterized by the enrichment of T cells, upregulated antigen presentation pathways, and enhanced immune signal interactions. Tissue microarray analysis further confirmed the presence of lymphoid lineages in the tumor stroma. Based on these findings, a hypothesis was proposed that the TME of adenoma and carcinoma in colorectal tissue are comparable, while the transition from benign to malignant epithelium is likely driven by changes in genomic mutations or/and expression.
FRONTIERS IN ONCOLOGY
(2022)
Article
Agronomy
Dongjin Qing, Guofu Deng, Yinghua Pan, Lijun Gao, Haifu Liang, Weiyong Zhou, Weiwei Chen, Jingcheng Li, Juan Huang, Ju Gao, Chunju Lu, Hao Wu, Kaiqiang Liu, Gaoxing Dai
Summary: By using the iTRAQ proteomics approach, we investigated the response of rice to low temperature stress. In this study, we detected changes in multiple proteins and found that the regulated proteins are mainly involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites, and carbon metabolism. Additionally, we observed that mRNA levels do not always correspond to protein levels. This study provides new insights into cold stress responses in rice with respect to proteomics and identifies candidate genes for cold-tolerance rice breeding.
Article
Plant Sciences
Liqing Lu, Haijian Yang, Yanhui Xu, Li Zhang, Juxun Wu, Hualin Yi
Summary: This study used RNA-seq and Pacbio-seq technologies to analyze the transcriptome and plant hormone signals in citrus seed development, revealing the important roles of hormone signaling, cell division, and nutrient metabolism processes. Additionally, several genes, including CrWRKY74, were found to play critical roles in seed abortion.
