☆ 4.5 Article

2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

ELECTROPHORESIS (2013)

期刊

ELECTROPHORESIS

卷 34, 期 4, 页码 505-509

出版社

WILEY-BLACKWELL

DOI: 10.1002/elps.201200256

关键词

Fungal proteomics; Genome annotation; Proteome reference map; Trichothecene

类别

Biochemical Research Methods Chemistry, Analytical

资金

Fonds National de la Recherche, Luxembourg [FNR/SECAL/07/02]

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摘要

Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.

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