期刊
ELECTROPHORESIS
卷 33, 期 9-10, 页码 1397-1401出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201100693
关键词
bathophenanthroline; Fluorescent stains; Iridium(III) complexes; SDS-PAGE
资金
- National Natural Science Foundations of China [20975074, 21072218]
- Innovation Fund for Technology Based Firms [09C26213203747]
This article reports a new class of luminescent metal complexes, biscyclometalated iridium(III) complexes with an ancillary bathophenanthroline disulfonate ligand, for staining protein bands that are separated by electrophoresis. The performances of these novel staining agents have been studied in comparison with tris(bathophenanthroline disulfonate) ruthenium(II) tetrasodium salt (i.e. RuBPS) using a commercially available imaging system. The staining agents showed different limits of detection, linear dynamic ranges, and protein-to-protein variations. The overall performances of all three stains were found to be better than or equivalent to RuBPS under the experimental conditions.
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