4.5 Article

Sequential microfluidic droplet processing for rapid DNA extraction

期刊

ELECTROPHORESIS
卷 32, 期 23, 页码 3399-3405

出版社

WILEY-BLACKWELL
DOI: 10.1002/elps.201100078

关键词

DNA extraction; Droplet processing; Droplet splitting; Reagent addition

资金

  1. 973 Program [2007CB714505, 2007CB714507]
  2. CAS [KJCX2-YW-H18, YZ200908]

向作者/读者索取更多资源

This work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for ?-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.

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