期刊
ELECTROPHORESIS
卷 32, 期 16, 页码 2091-2098出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201000657
关键词
Alzheimer's disease; Enrichment; Low-abundance protein; Functionalized magnetic nanoparticles; Serum amyloid P component
资金
- National Nature Science Foundation of China [20975016, 21005007]
- Major State Basic Research Program of China [2006CB910101]
- Beijing Natural Science Foundation [2093036]
- Specialized Research Fund for the Doctoral Program of Higher Education [200800270006, 20100003120014]
This paper describes the applications of multifunctional magnetic nanoparticles (MNPs) for the enrichment of low-abundance proteins for polyacrylamide gel electrophoresis (PAGE) separation. The hemoglobin-functionalized MNPs, named Hb-MNPs, were obtained based on electrostatic interactions and covalent binding between the hemoglobin (Hb) and the MNPs. It was demonstrated that the proteins in human serum were selectively conjugated to Hb-MNPs, which can be used for the selective enrichment of low-abundance proteins. Three and seven kinds of proteins were identified by MS after 1-D and 2-D PAGE, respectively. Comparing with native PAGE without the treatment of MNPs, some proteins were observed, such as human serum amyloid P component (SAP), vitamin D-binding protein, and serine peptidase inhibitor. Because the high concentration of SAP can be considered as a signal for the neurodegeneration of Alzheimer's disease, the present Hb-MNPs-based method was applied to investigate the serum level of SAP for the diagnosis of Alzheimer's disease, and the results are satisfying.
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