期刊
ELECTROPHORESIS
卷 32, 期 23, 页码 3392-3398出版社
WILEY-BLACKWELL
DOI: 10.1002/elps.201100362
关键词
Contamination; Fraction collection; Fractionation; Reconstruction; Simultaneous space sampling
资金
- Japan Science and Technology Agency
- Ministry of Education, Culture, Sports, Science, and Technology of Japan: KAKENHI [19049001, 23225006, 470]
- Hokkaido Innovation through Nanotechnology Support (HINTS)
- Grants-in-Aid for Scientific Research [23225006, 23686026, 23659862] Funding Source: KAKEN
Fraction collection of selected components from a complex mixture plays a critical role in biomedical research, environmental analysis, and biotechnology. Here, we introduce a novel electrophoretic chip device based on a signal processing theorem that allows simultaneous space sampling for fractionation of ssDNA target fragments. Ten parallel extraction channels, which covered 1.5-mm-long sampling ranges, were used to facilitate the capturing of fast-moving fragments. Furthermore, the space sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Fragments of 180, 181, and 182 bases were simultaneously collected, and then the recovered DNA was PCR amplified and assessed by CE analysis. The 181-base target was shown to be isolated in a 70-mm-long separation length within 10?min, in contrast to the >50?min required for the 300-mm-long separation channel in our previous study. This method provides effective combination of time and space, which is a breakthrough in the traditional concept of fraction collection on a chip.
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