Article
Pharmacology & Pharmacy
Yong Deng, Jing Zhao, Shaoping Li
Summary: Polysaccharides are important for biomedical and functional food applications, but their quantification is challenging due to their complex structure and lack of standards. This study proposes an improved method using saccharide mapping to assess polysaccharides from Hericium erinaceus. The method involves digestion, labeling, and separation of specific oligosaccharides, resulting in accurate quantification. The proposed method can enhance the quality control of polysaccharides, benefiting not only H. erinaceus but also other materials.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Xiao Yu, Wanxiang Yu, Xiaohui Zhang, Yongjie Wang, Shumei Wang, Haiyun Zhai
Summary: A new solid-phase extraction (SPE) coupled with capillary electrophoresis (CE) method was developed for preconcentration and determination of anthraquinones and flavonoids in honey. By optimizing extraction parameters and using chitin as adsorbent, the method achieved enrichment and detection of target compounds with high sensitivity.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yusuke Gonda, Akina Matsuda, Kenichiro Adachi, Chiharu Ishii, Masataka Suzuki, Akina Osaki, Masashi Mita, Naoto Nishizaki, Yoshiyuki Ohtomo, Toshiaki Shimizu, Masato Yasui, Kenji Hamase, Jumpei Sasabe
Summary: Mammals maintain systemic dominance of L-amino acids through enzymatic degradation and excretion of D-amino acids. The majority of microbial D-amino acids are catabolized by host enzymes, while urinary excretion plays a minor role. This active regulation of amino acid homochirality depends on maternal catabolism during the prenatal period and switches to host catabolism after birth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Beryl X. Li, Daniel K. Kim, Steven Bloom, Richard Y. -C. Huang, Jennifer X. Qiao, William R. Ewing, Daniel G. Oblinsky, Gregory D. Scholes, David W. C. MacMillan
Summary: This study presents a novel method for site-selective tyrosine bioconjugation using photoredox catalysis, which allows for chemically modifying native proteins with high site-selectivity. The water-soluble photocatalyst lumiflavin has been demonstrated to induce oxidative coupling between a phenoxazine dialdehyde tag and a single tyrosine site on native proteins, even in the presence of multiple tyrosyl side chains, enabling the synthesis of structurally defined fluorescent conjugates.
Article
Biochemical Research Methods
Hai Yen Ta, Lucie Perquis, Cedric Sarazin, Bruno Guiard, Varravaddheay Ong Meang, Fabrice Collin, Francois Couderc
Summary: The study utilized capillary electrophoresis coupled to LED-induced fluorescence detection and CBQCA labeling for the analysis of amino acids such as tryptophan, facing challenges in detection and quantification, especially for Trp. Another approach, CE/capacitively coupled contactless conductivity detection, showed better performance for quantification of Trp, Tyr, Val, and Ile, with a LOD of 2 μM for Trp.
Article
Chemistry, Multidisciplinary
Guoxiu Wang, Wenjuan Lv, Congjie Pan, Hongli Chen, Xingguo Chen
Summary: A novel chiral COF, DA-TD COF, was synthesized and used as a chiral stationary phase for open-tubular capillary electrochromatography, showing excellent enantioseparation efficiency and stability. The enantioseparation mechanism was also studied in this work.
CHEMICAL COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Gregory Gauthier-Coles, Angelika Broer, Malcolm Donald McLeod, Amee J. George, Ross D. Hannan, Stefan Broer
Summary: SNAT2 is an important amino acid transporter involved in amino acid accumulation, cellular osmolarity, and cell growth. A potent inhibitor of SNAT2 has been identified through high-throughput screening, with selectivity against other transporters. Combined with a glucose transport inhibitor, this compound can halt the proliferative growth of cancer cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Fawei Zhu, Tianqi Yuan, Wang Jing, Kai Chen, Lumin Wang, Qiachi Fu, Jianping Guan, Miao Chen, Qi Liu, Hua Yang, Xiaoqing Chen
Summary: A low-cost, high-throughput photoresponsive strategy for the sensing of amino acid enantiomers was proposed, utilizing the oxidation reactions of amino acid oxidase and substrate with an aromatic boronic ester. The method demonstrated sensitive detection of D/L-AA concentrations through the colorimetric and fluorescent response of the probe HBT-PB to H2O2. The practicality and adaptability of the scheme were confirmed by accurate analysis of methionine and Dopa samples, highlighting its potential for high-throughput screening and sensitive analysis.
Article
Endocrinology & Metabolism
Shuiya Sun, Dongjuan He, Cheng Luo, Xihua Lin, Jiahua Wu, Xueyao Yin, Chengfang Jia, Qianqian Pan, Xuehong Dong, Fenping Zheng, Hong Li, Jiaqiang Zhou
Summary: Recent studies have shown that plasma amino acid levels are associated with visceral obesity, insulin resistance, and future development of diabetes and cardiovascular diseases. This study aimed to examine the association between amino acid profile and metabolic syndrome in the Chinese Han population. The results indicated that MetS patients exhibit an abnormal amino acid profile, with certain amino acids showing significant differences between MetS and non-MetS groups. Additionally, decreased taurine levels may be linked to the future development of MetS.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Analytical
Shaoyan Zhang, Jinfeng Ning, Qingqing Wang, Wei Wang
Summary: Flavonoids are widely used in the treatment of various diseases due to their antioxidant, anti-inflammatory, anticancer and antiviral properties. In this study, a method of fluorescence enhancement of flavonoids was introduced for the first time using sodium acetate for flavonoid derivatization. Five specific flavonoids were successfully separated and analyzed by capillary electrophoresis with laser-induced fluorescence detection. The developed method was then applied to the determination of flavonoids in traditional Chinese medicines, and the presence of flavonoids was confirmed in all tested medicines. The method proved to be fast, sensitive and reliable for the determination of flavonoids.
ANALYTICAL METHODS
(2023)
Article
Plant Sciences
Bin Zhou, Prabhakar S. Achanta, Gauri Shetye, Shao-Nong Chen, Hyun Lee, Ying-Yu Jin, Jinhua Cheng, Mi-Jin Lee, Joo-Won Suh, Sanghyun Cho, Scott G. Franzblau, Guido F. Pauli, James B. McAlpine
Summary: The paper discusses the structures, naming, and anti-Mycobacterium tuberculosis activity of cyclopeptides such as rufomycin and ilamycin. Through NMR spectroscopy, the configuration and diastereoisomers of these compounds were determined, and semisynthetic derivatives were prepared for validation. Structural differences and conformers were analyzed to understand the biological activity of these compounds, leading to a unified nomenclature for the group.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Plant Sciences
Julien Besnard, Unnati Sonawala, Bal Maharjan, Eva Collakova, Scott A. Finlayson, Guillaume Pilot, John McDowell, Sakiko Okumoto
Summary: Amino acids play important roles in biosynthesis and signaling, affecting plant development and immune responses. Changes in amino acid levels can trigger stress phenotypes and pathogen resistance through various pathways, indicating a link between amino acid transport and stress responses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Nutrition & Dietetics
Alyssa Paoletti, Paul B. Pencharz, Ronald O. Ball, Dehan Kong, Libai Xu, Rajavel Elango, Glenda Courtney-Martin
Summary: This study determined the total sulfur amino acid (TSAA) requirement in older adults and found that the requirement is significantly higher in older men compared to older women.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Agronomy
Tianyi Wang, Yunfei Li, Yumin Huang, Xiaoming Zhao, Zhaobin Dong, Weiwei Jin, Wei Huang
Summary: This study identified the positive regulatory role of ZmAAP6, a maize gene, in grain protein content (GPC) regulation. Mutant and overexpression analysis revealed its involvement in protein body formation and amino acid transportation.
Article
Biochemistry & Molecular Biology
Ya Chun Yu, Jung Min Han, Sunghoon Kim
Summary: Aminoacyl-tRNA synthetases play essential roles in protein synthesis and recent studies have revealed their previously unknown biological functions beyond catalytic roles. Sensing intracellular nutrients is crucial for cell physiology, and certain types of ARSs may be involved in sensing and signaling their cognate amino acids within cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Biochemical Research Methods
Francois Couderc, Varravaddheay Ong-Meang, Verena Poinsot
Article
Biochemical Research Methods
Audrey Ric, Vincent Ecochard, Jason S. Iacovoni, Audrey Boutonnet, Frederic Ginot, Varravaddheay Ong-Meang, Verena Poinsot, Laurent Paquereau, Francois Couderc
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Yuan Hui Liu, Yin Shan Jiao, Li Xue Liu, Dan Wang, Chang Fu Tian, En Tao Wang, Lei Wang, Wen Xin Chen, Shang Ying Wu, Bao Lin Guo, Zha Gen Guan, Verena Poinsot, Wen Feng Chen
MOLECULAR PLANT-MICROBE INTERACTIONS
(2018)
Review
Biochemical Research Methods
Verena Poinsot, Hai Yen Ta, Varravaddheay Ong Meang, Lucie Perquis, Pierre Gavard, Bernard Pipy, Francois Couderc
Article
Microbiology
Dan Wang, Francois Couderc, Chang Fu Tian, Wenjie Gu, Li Xue Liu, Verena Poinsot
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Oncology
Mohamad Alaeddine, Melissa Prat, Verena Poinsot, Valerie Gouaze-Andersson, Helene Authier, Etienne Meunier, Lise Lefevre, Camille Alric, Christophe Dardenne, Jose Bernad, Laurent Alric, Bruno Segui, Patricia Balard, Francois Couderc, Bettina Couderc, Bernard Pipy, Agnes Coste
CANCER IMMUNOLOGY RESEARCH
(2019)
Article
Plant Sciences
Fernando Sorroche, Mathilda Walch, Lan Zou, David Rengel, Fabienne Maillet, Chrystel Gibelin-Viala, Verena Poinsot, Christian Chervin, Catherine Masson-Boivin, Clare Gough, Jacques Batut, Anne-Marie Garnerone
Article
Biochemical Research Methods
Lucie Perquis, Hai Yen Ta, Varravaddheay Ong-Meang, Alix Poinso, Fabrice Collin, Verena Poinsot, Francois Couderc
Article
Biochemistry & Molecular Biology
Benoit Daubech, Verena Poinsot, Agnieszka Klonowska, Delphine Capela, Clemence Chaintreuil, Lionel Moulin, Marta Marchetti, Catherine Masson-Boivin
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
Article
Multidisciplinary Sciences
Tomas Allen Rush, Virginie Puech-Pages, Adeline Bascaules, Patricia Jargeat, Fabienne Maillet, Alexandra Haouy, Arthur QuyManh Maes, Cristobal Carrera Carriel, Devanshi Khokhani, Michelle Keller-Pearson, Joanna Tannous, Kevin R. Cope, Kevin Garcia, Junko Maeda, Chad Johnson, Bailey Kleven, Quanita J. Choudhury, Jessy Labbe, Candice Swift, Michelle A. O'Malley, Jin Woo Bok, Sylvain Cottaz, Sebastien Fort, Verena Poinsot, Michael R. Sussman, Corinne Lefort, Jeniel Nett, Nancy P. Keller, Guillaume Becard, Jean-Michel Ane
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Nathalie Pizzinat, Varravaddheay Ong-Meang, Florence Bourgailh-Tortosa, Muriel Blanzat, Lucie Perquis, Daniel Cussac, Angelo Parini, Verena Poinsot
Review
Biochemical Research Methods
Verena Poinsot, Varravaddheay Ong-Meang, Audrey Ric, Pierre Gavard, Lucie Perquis, Francois Couderc
