Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Article
Chemistry, Analytical
Yuyan Xue, Haimei Shi, Bin Feng, Liang Qiao, Chuanfan Ding, Shaoning Yu
Summary: In this study, bacteria in blood culture bottles were enriched using co-magnetic beads for MALDI-TOF MS profiling. Compared to single magnetic beads, co-magnetic beads showed better bacterial capture efficiency, decreasing false-negative results. This proposed strategy is more suitable for enriching clinically unknown bacteria from blood culture bottles for MALDI-TOF MS database identification.
Article
Biochemical Research Methods
Ashley Di Meo, Dorsa Sohaei, Ihor Batruch, Pantelis Alexandrou, Ioannis Prassas, Eleftherios P. Diamandis
Summary: The analysis utilized LC-MS/MS technology to profile the normal human proteome from 117 samples across 46 normal tissues and organs, quantifying 10,438 unique proteins. By combining data from biological fluid proteomics of healthy individuals, tandem mass spectra corresponding to 13,028 unique human protein-coding genes were generated, serving as an important complementary resource for future biomarker discovery.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Immunology
Xiaoyue He, Donghua Liu, Qicong Chen
Summary: The study identified the significant role of heat shock protein HSP90 in the melanin synthesis pathway of T.marneffei through proteomic analysis. The HSP90 inhibitor geldanamycin demonstrated effective inhibition of melanin production in T.marneffei yeast.
MICROBIAL PATHOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Yajun Hu, Jun Yao, Zening Wang, Hui Liang, Cunyu Li, Xinwen Zhou, Fengying Yang, Yang Zhang, Hong Jin
Summary: Trichosanthin (TCS), a Chinese herbal medicine, has been used in the treatment of gynecological diseases. In this study, the anticancer properties of TCS were tested using the human trophoblast cell line BeWo. MTT method was used for primary screening, and proteomics analysis was conducted to investigate the drug effect. The study identified several differentially expressed proteins and suggested potential pathways and signal transduction for disease treatment.
Article
Endocrinology & Metabolism
Hicham Benabdelkamel, Mohamed Rafiullah, Afshan Masood, Abdulaziz Alsaif, Mohthash Musambil, Assim A. Alfadda
Summary: This study used proteomics approach to analyze the alterations in the thyroid tissue proteome in patients with benign diffuse goiter. The results showed significant changes in the expression of tissue proteins, particularly in signaling pathways and metabolic pathways.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Severin A. Korfhage, Sven Rossel, Saskia Brix, Catherine S. McFadden, Steinunn Hilma Olafsdottir, Pedro Martinez Arbizu
Summary: This study tested a fast and cheap species delimitation tool for Octocorallia and Scleractinia in the Northeast Atlantic. The results showed that genetic methods could distinguish species relatively well, while proteomic fingerprinting only achieved limited success due to a lack of replicates. It is predicted that with the establishment of a reference library, proteomic fingerprinting could provide a rapid and cost-effective alternative for species discrimination in corals.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Applied
Ling Ling, Liyan Jiang, Qirui Chen, Bo Zhao, Yueying Li, Xinhua Guo
Summary: A rapid and highly reproducible method for profiling oligosaccharides in beer using PAPAN reactive matrix and MALDI-TOF MS was reported. Through optimization of sample dilution factor and cationization agents, successful identification of maltodextrin distributions in different brands of beers was achieved, showing potentials for analysis of oligosaccharides in other foods.
Article
Plant Sciences
Swetha Sudha Nagamalla, Malini Devi Alaparthi, Sunitha Mellacheruvu, Ravindar Gundeti, Jana Priya Sony Earrawandla, Someswar Rao Sagurthi
Summary: The study investigated the impact of drought stress on two cotton varieties, revealing that Bt cotton showed stronger drought tolerance compared to non-Bt cotton at the protein expression and physiological levels. This finding may aid in breeding new drought-tolerant and high-yielding cotton hybrid varieties.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
Liang Shan, Han Gao, Jing Zhang, Wentao Li, Yue Su, Yinlong Guo
Summary: This study combined MALDI-TOF MS with EM technology to directly analyze exosomal proteins in human plasma and serum. The results showed that EM had higher sensitivity in the high-mass range and characteristic exosomal proteins were identified. Furthermore, when using EM as a detector, there was a good linear relationship between peak intensity and concentration.
Article
Chemistry, Applied
Rocio Galindo-Lujan, Laura Pont, Victoria Sanz-Nebot, Fernando Benavente
Summary: This study presents a method using mass spectrometry to analyze quinoa proteins, allowing for accurate identification of different commercial quinoa varieties. The developed method provides reliable protein fingerprints for classification of quinoa products based on their protein profiles.
Article
Chemistry, Applied
Xue Yi Tan, Azizah Misran, Leona Daniela Jeffery Daim, Benjamin Yii Chung Lau
Summary: Four methods were evaluated to extract proteins from Musang King durian pulps, with acetone-phenol method producing the best results. Differential protein analyses revealed that 15 proteins were down-regulated and 3 proteins were up-regulated after a year of frozen storage. This study indicates that frozen storage affects protein expression in various functional aspects.
Article
Immunology
Peng Liu, Jianqiang Wu, Dandan Sun, Haolong Li, Zhihong Qi, Xiaoyue Tang, Wei Su, Yongzhe Li, Xuzhen Qin
Summary: This study aimed to identify and validate candidate protein biomarkers for cryoglobulinemia (CGE) using a TMT-PRM-ELISA workflow. APOA1 and PCSK9 were confirmed to be increased in CGE patients, indicating the involvement of proteins in cholesterol metabolism in the development of CGE. These findings provide valuable insights for pathogenesis research and biomarker discovery in CGE.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Hannah S. J. Choi, Grishma Hirode, Chien-Hung Chen, Tung-Hung Su, Wai-Kay Seto, Stijn Van Hees, Margarita Papatheodoridi, Sabela Lens, Grace L. H. Wong, Sylvia M. Brakenhoff, Rong-Nan Chien, Jordan J. Feld, Milan J. Sonneveld, Henry L. Y. Chan, Xavier Forns, George Papatheodoridis, Thomas Vanwolleghem, Man-Fung Yuen, Yao-Chun Hsu, Jia-Horn Kao, Markus Cornberg, Bettina E. Hansen, Wen-Jue Jeng, Harry L. A. Janssen
Summary: This study compared the relapse rates and treatment outcomes after discontinuation of therapy in chronic hepatitis B patients using entecavir (ETV) and tenofovir disoproxil fumarate (TDF). It found that TDF was associated with a higher rate of HBsAg loss, earlier virological relapse, and higher clinical relapse compared to ETV. However, the rates of HBsAg loss and retreatment were similar between the two treatment groups. Finite therapy can be considered for CHB patients on either TDF or ETV therapy.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Gastroenterology & Hepatology
Huapeng Lin, Terry Cheuk-Fung Yip, Xinrong Zhang, Guanlin Li, Yee-Kit Tse, Vicki Wing-Ki Hui, Lilian Yan Liang, Jimmy Che-To Lai, Stephen Lam Chan, Henry Lik-Yuen Chan, Grace Lai-Hung Wong, Vincent Wai-Sun Wong
Summary: The relative importance of liver-related death increases with age in patients with NAFLD, especially among women.
Article
Gastroenterology & Hepatology
Harry L. A. Janssen, Jinlin Hou, Tarik Asselah, Henry L. Y. Chan, Fabien Zoulim, Yasuhito Tanaka, Ewa Janczewska, Ronald G. Nahass, Stefan Bourgeois, Maria Buti, Pietro Lampertico, Oliver Lenz, Thierry Verbinnen, Joris Vandenbossche, Willem Talloen, Ronald Kalmeijer, Maria Beumont, Michael Biermer, Umesh Shukla
Summary: The study evaluated the efficacy and safety of JNJ-56136379 in patients with chronic hepatitis B. The results showed that JNJ-56136379+NA significantly reduced HBV DNA and HBV RNA levels, but had limited effects on HBsAg or HBeAg declines in HBeAg-positive patients. Overall, JNJ-56136379 did not demonstrate clear superiority over NA in terms of efficacy.
Article
Gastroenterology & Hepatology
Terry Cheuk-Fung Yip, Vincent Wai-Sun Wong, Mandy Sze -Man Lai, Jimmy Che-To Lai, Vicki Wing-Ki Hui, Lilian Yan Liang, Yee-Kit Tse, Henry Lik-Yuen Chan, Grace Lai -Hung Wong
Summary: We investigated the long-term incidence of hepatocellular carcinoma (HCC) and hepatic decompensation among chronic hepatitis B patients who have achieved hepatitis B surface antigen (HBsAg) seroclearance. The study found that the proportion of patients who developed HCC after HBsAg clearance was 1.1%, while the proportion of patients who developed hepatic decompensation was 1.3%. HBsAg clearance for over 7 years was associated with a reduced risk of hepatic decompensation but not HCC.
JOURNAL OF HEPATOLOGY
(2023)
Review
Gastroenterology & Hepatology
Do Young Kim, Bao Nguyen Toan, Chee-Kiat Tan, Irsan Hasan, Lyana Setiawan, Ming -Lung Yu, Namiki Izumi, Nguyen Nguyen Huyen, Pierce Kah-Hoe Chow, Rosmawati Mohamed, Stephen Lam Chan, Tawesak Tanwandee, Teng-Yu Lee, Thi Thanh Nguyen Hai, Tian Yang, Woo -Chang Lee, Henry Lik Yuen Chan
Summary: PIVKA-II is valuable for detecting AFP-negative hepatocellular carcinoma (HCC), and could be used in combination with AFP for early HCC detection. It is clinically useful for monitoring HCC treatment, predicting recurrence and microvascular invasion risk, and facilitating patient selection for liver transplant. However, more evidence is needed for its role in surveillance, including small HCC.
CLINICAL AND MOLECULAR HEPATOLOGY
(2023)
Article
Gastroenterology & Hepatology
Ada S. F. Chan, Henry L. Y. Chan, Bruno K. L. Yan, Mooris K. C. Lai
Summary: A study conducted in a private hospital in Hong Kong found that the use of ATP bioluminescence assay is a rapid and cost-effective method for evaluating the presence of microbial residue on endoscopes and monitoring their cleanliness.
Article
Gastroenterology & Hepatology
Xinrong Zhang, Terry Cheuk-Fung Yip, Yee-Kit Tse, Vicki Wing-Ki Hui, Guanlin Li, Huapeng Lin, Lilian Yan Liang, Jimmy Che-To Lai, Mandy Sze-Man Lai, Johnny T. K. Cheung, Henry Lik-Yuen Chan, Stephen Lam Chan, Alice Pik-Shan Kong, Grace Lai-Hung Wong, Vincent Wai-Sun Wong
Summary: This study found that risk factor control and treatment improved significantly in patients with NAFLD and T2D from 2000 to 2020, with improved glycemic and lipid control but no significant change in blood pressure control.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Gastroenterology & Hepatology
Kenneth W. W. Lin, Rajneesh Kumar, Feng Shen, Henry L. -Y. Chan, Grace L. -H. Wong, Rahul Kumar, Wan Cheng Chow, Su Lin, Vincent W. -S. Wong, Jian-Gao Fan, George B. -B. Goh
Summary: The prevalence of concomitant chronic hepatitis B (CHB) and hepatic steatosis (HS) is increasing in Asia. Non-invasive tests (NITs) have good accuracy in predicting fibrosis degree in patients with concomitant CHB and HS.
LIVER INTERNATIONAL
(2023)
Article
Oncology
Claudia Wing-Kwan Wu, Rashid Nok-Shun Lui, Vincent Wai-Sun Wong, Tsz-Fai Yam, Terry Cheuk-Fung Yip, Ken Liu, Jimmy Che-To Lai, Yee-Kit Tse, Tony Shu-Kam Mok, Henry Lik-Yuen Chan, Kelvin Kwok-Chai Ng, Grace Lai-Hung Wong, Stephen Lam Chan
Summary: The study successfully validated the efficacy of the Baveno VII criteria in predicting high-risk varices in patients with hepatocellular carcinoma, providing a non-invasive method for assessing the risk of variceal bleeding and hepatic decompensation.
Article
Gastroenterology & Hepatology
Xinrong Zhang, Terry Cheuk-Fung Yip, Grace Lai-Hung Wong, Wei-Xuan Leow, Lilian Yan Liang, Lee-Ling Lim, Guanlin Li, Luqman Ibrahim, Huapeng Lin, Jimmy Che To Lai, Angel Mei-Ling Chim, Henry Lik Yuen Chan, Alice Pik-Shan Kong, Wah Kheong Chan, Vincent Wai-Sun Wong
Summary: The study aimed to investigate whether automated fibrosis score calculation and electronic reminder messages could increase the detection of advanced liver disease in patients with type 2 diabetes. The results showed that in the intervention group using automated fibrosis score calculation and electronic reminders, 33.3% of patients received appropriate care, compared to only 3.1% in the control group.
Meeting Abstract
Gastroenterology & Hepatology
Che-To Lai, Vincent Wai-Sun Wong, Angel Mei-Ling Chim, Vicki Wing-Ki Hui, Yee-Kit Tse, Terry Cheuk-Fung Yip, Henry Lik-Yuen Chan, Grace Lai-Hung Wong
Meeting Abstract
Gastroenterology & Hepatology
Che-To Lai, Vincent Wai-Sun Wong, Vicki Wing-Ki Hui, Yee-Kit Tse, Terry Cheuk-Fung Yip, Henry Lik-Yuen Chan, Grace Lai-Hung Wong
Meeting Abstract
Gastroenterology & Hepatology
Yan Liang, Terry Cheuk-Fung Yip, Jimmy Che-To Lai, Amy Shuk-Man Lam, Yee-Kit Tse, Vicki Wing-Ki Hui, Henry Lik-Yuen Chan, Vincent Wai-Sun Wong
Meeting Abstract
Gastroenterology & Hepatology
Terry Cheuk-Fung Yip, Wayne Yau-Wang Hui, Wilfred Wang-Fung Ho, Zackary Kwun-Lam Tsang, Tsz-Fai Yam, Mandy Sze-Man Lai, Yee-Kit Tse, Henry Lik-Yuen Chan, Grace Lai-Hung Wong, Vincent Wai-Sun Wong
Meeting Abstract
Gastroenterology & Hepatology
Grishma Hirode, Bettina Hansen, Chien-Hung Chen, Tung-Hung Su, Margarita Papatheodoridi, Sabela Lens, Stijn Van Hees, Sylvia Brakenhoff, Hannah S. J. Choi, Rong-Nan Chien, Wai-Kay Seto, Grace Wong, Jordan Feld, Henry L. Y. Chan, Man-Fung Yuen, Milan Sonneveld, Thomas Vanwolleghem, Xavier Forns, George Papatheodoridis, Jia-Horng Kao, Yao-Chun Hsu, Markus Cornberg, Rachel Wen-Juei Jeng, Harry Janssen
JOURNAL OF HEPATOLOGY
(2022)
