Review
Pharmacology & Pharmacy
Renata Maia, Violeta Carvalho, Rui Lima, Graca Minas, Raquel O. Rodrigues
Summary: This systematic review summarizes the recent progress in the use of microneedles (MNs) in biomedical applications, including drug delivery, biomarker detection, and their potential integration with microfluidic devices. Although MNs have been extensively utilized in lab-on-a-chip platforms, recent studies have also explored their applicability in organ-on-a-chip models. Overall, the presence of MNs in advanced microfluidic devices offers simplified drug delivery, microinjection, and biomarker detection through integrated biosensors, enabling real-time monitoring of various biomarkers in lab- and organ-on-a-chip platforms.
Article
Chemistry, Analytical
Mahesh P. Bhat, Mahaveer Kurkuri, Dusan Losic, Madhuprasad Kigga, Tariq Altalhi
Summary: A PDMS microfluidic channel integrated with a UV-vis fibre-optic spectrometer and a new colorimetric probe was developed for sensitive and real-time quantitative measurements of fluoride ions (F). The device features an 'S' shaped microchannel acting as a microreactor to enhance the reaction between the colorimetric probe and F ions. This optofluidic device demonstrates high sensitivity and selectivity for F ions, outperforming conventional methods in detecting real samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Electrical & Electronic
Sarah E. Lu, Andrew Morris, Geraldine Clinton-Bailey, Medya Namiq, Paul C. Gow, Antony Birchill, Sebastian Steigenberger, James Wyatt, Reuben Forrester, Matthew C. Mowlem, Phillip E. Warwick
Summary: Nuclear reactor process control often relies on manual sampling and laboratory analysis for monitoring pure β-emitting radionuclides, leading to delays in data availability and response times. The development of an in situ microfluidic Lab on Chip (LoC) system with integrated detection offers a promising solution, reducing occupational exposure and monitoring costs while providing improved temporal resolution through near real-time data acquisition.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Review
Pharmacology & Pharmacy
Ghazal Shabestani Monfared, Peter Ertl, Mario Rothbauer
Summary: Cutaneous wound healing is a complex process involving cell communication events and cell migration to achieve wound closure. Microfluidic and lab-on-a-chip technologies have improved in vitro wound healing assays to create more reliable models resembling the in vivo wound microenvironment.
Article
Biophysics
Xu-Guang Hu, Yong Zhao, Yun Peng, Xiao-Ming Chen, Lu-Feng Wang, Zi-Ting Lin, Jian Zhao, Sheng Hu
Summary: We demonstrated a temperature-compensated optofluidic DNA biosensor for microfluidic chip. The biosensor composed of an interferometer and a fiber Bragg grating achieved specific detection of single stranded complementary DNA. The sensor could also monitor temperature and achieve fast sample replacement with low sample consumption. This optofluidic DNA biosensor has great significance in DNA hybridization research and biological analysis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Electrical & Electronic
Aleksandra Pokrzywnicka, Patrycja Sniadek, Rafal Walczak
Summary: This paper presents an integrated microprism matrix for light coupling and optical sensing systems, fabricated using controlled negative pressure glass thermal reflow process. The controlled coupling of the beam into a substrate was achieved through determination of optimal geometric dimensions of microprisms and configuration of a measurement setup. Fluorimetric tests demonstrated the potential use of a microprism matrix for 500 nm long-pass detection in lab-on-a-chip platforms.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2021)
Article
Computer Science, Theory & Methods
Xing Huang, Tsung-Yi Ho, Wenzhong Guo, Bing Li, Krishnendu Chakrabarty, Ulf Schlichtmann
Summary: Flow-based microfluidic biochips are increasingly utilized in biology and biochemistry for laboratory procedures, with advancements in manufacturing technology allowing for integration of thousands of microvalves into a small platform. Computer-aided design (CAD) in the past decade has attracted research interest for automation of biochip design, enabling designers to explore new chip architectures and simplify complex tasks. Key trends and directions for future research focus on maximizing the potential of microfluidics to advance the industry.
ACM COMPUTING SURVEYS
(2021)
Article
Optics
Riccardo Zamboni, Ludovic Gauthier-Manuel, Annamaria Zaltron, Liana Lucchetti, Mathieu Chauvet, Cinzia Sada
Summary: This work presents a reconfigurable opto-microfluidic coupling between optical waveguides and tilted microfluidic channels in monolithic lithium niobate crystal. The light path connecting two waveguide arrays located on opposite sides of a microfluidic channel depends on the refractive index between the liquid phase and the hosting crystal. Proof-of-concept applications are herein presented, including microfluidic optical waveguide switching, optical refractive index sensing, and wavelength demultiplexing.
Review
Chemistry, Analytical
Yu-Jia Wei, Ya-Nan Zhao, Xuan Zhang, Xing Wei, Ming-Li Chen, Xu-Wei Chen
Summary: Nowadays, biochemical analysis, such as cell analysis, disease diagnosis and treatment, environmental monitoring, and food safety, is receiving increasing attention. Optically integrated microfluidic technology combines the benefits of high sensitivity, fast detection, and excellent selectivity. By utilizing flexible optical detection techniques and precisely-controlled fluidics, microfluidic chips enable high throughput analysis, reduced reagent consumption, and decreased assay expenditure. This review introduces the integration of microfluidic chips with various optical detection techniques and their applications in biochemical analysis, and also discusses the future prospects of optically integrated microfluidic chips.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Luis Abraham Garcia-Hernandez, Eduardo Martinez-Martinez, Denni Pazos-Solis, Javier Aguado-Preciado, Ateet Dutt, Abraham Ulises Chavez-Ramirez, Brian Korgel, Ashutosh Sharma, Goldie Oza
Summary: This paper reviews recent developments in the detection of cancer cells using the microfluidics-based lab-on-a-chip method, with a focus on techniques that use optical phenomena and various probes for sensing, amplification, and immobilization. The paper also discusses current challenges and prospects for cancer diagnosis.
Article
Engineering, Electrical & Electronic
Jens Ducree
Summary: Non-genuine medical products, including diagnostic devices, have become a lucrative business for fraudsters, causing significant damage to revenues and reputation of companies, as well as posing a significant risk to the health of people and societies.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Engineering, Multidisciplinary
Tong Hou, Hui Chang, Haoliang Jiang, Pengcheng Wang, Na Li, Yongxin Song, Dongqing Li
Summary: The smartphone integrated microfluidic lab-on-chip device enables real-time detection, counting, and sizing of living algae in ship's ballast water, achieving high accuracy and convenience in operation.
Article
Biophysics
Stefanie Fuchs, Veronika Rieger, Anders O. Tjell, Sarah Spitz, Konstanze Brandauer, Roland Schaller-Ammann, Juergen Feiel, Peter Ertl, Ingo Klimant, Torsten Mayr
Summary: A miniaturized enzymatic, optical glucose sensor element was developed for stable glucose measurements inside microfluidic systems, providing valuable information on the viability and metabolic state of cultured cells.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Electrical & Electronic
Juul Goossens, Thijs Vandenryt, Joseph W. W. Lowdon, Bart van Grinsven, Kasper Eersels, Wim Deferme, Ronald Thoelen
Summary: Molecular imprinted polymers (MIPs) are used for their selective recognition of different analytes, but the immobilization process poses challenges. This research introduces dye-displacement MIPs to enhance their application potential. A disposable lab-on-chip cartridge is used to allow MIPs to interact with the target solution, coupled to a portable optical sensor setup for quick screening of samples. The setup has the capability to identify different color dyes and intensities, and can be used for various sensing applications including food quality assessment, environmental testing, and pharmaceutical analysis.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Review
Chemistry, Analytical
Federico Sala, Carlotta Ficorella, Roberto Osellame, Josef A. Kaes, Rebeca Martinez Vazquez
Summary: Understanding cell migration is crucial for unraveling physiological phenomena and predicting pathologies like cancer metastasis. Microfluidic devices have been used to investigate cellular migration response by engineering mechanical and spatial stimuli. This review discusses the existing technologies, such as soft lithography and femtosecond laser micromachining, used in microfluidic cellular migration assays. It highlights the different geometrical configurations employed and envisions future developments in in vitro migration studies under spatial confinement in microfluidic devices.
Article
Materials Science, Multidisciplinary
Reka Geczy, Monica Agnoletti, Mikkel F. Hansen, Jorg P. Kutter, Katayoun Saatchi, Urs O. Hafeli
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2019)
Article
Chemistry, Analytical
Rasmus R. Svejdal, Eleanor R. Dickinson, Drago Sticker, Jorg P. Kutter, Kasper D. Rand
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Cristina Roman-Hidalgo, Elia Santigosa-Murillo, Maria Ramos-Payan, Nickolaj J. Petersen, Joerg P. Kutter, Stig Pedersen-Bjergaard
Review
Nanoscience & Nanotechnology
Drago Sticker, Reka Geczy, Urs O. Hafeli, Jorg P. Kutter
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Biochemical Research Methods
Nan Lu, Drago Sticker, Andreas Kretschmann, Nickolaj J. Petersen, Jorg P. Kutter
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Review
Biochemical Research Methods
Nan Lu, Jorg P. Kutter
Article
Chemistry, Analytical
Rasmus R. Svejdal, Drago Sticker, Claus Sonderby, Jorg P. Kutter, Kasper D. Rand
ANALYTICA CHIMICA ACTA
(2020)
Article
Biochemical Research Methods
Nan Lu, Nickolaj J. Petersen, Andreas C. Kretschmann, Jorg P. Kutter
Summary: Non-aqueous capillary electrophoresis on a thiol-ene microfluidic chip coupled with ESI-MS offers a versatile system for the analysis of lipophilic compounds in various organic solvents, showing good stability and separation efficiency. The integration of ESI emitter on the microchip demonstrated stable signal with high separation efficiency using ethanol-based buffers. The direct coupling of non-aqueous electrophoresis to mass spectrometry allows for fast analysis with good sensitivities for hydrophobic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Tullio V. F. Esposito, Helene Stutz, Cristina Rodriguez-Rodriguez, Marta Bergamo, Lovelyn Charles, Reka Geczy, Colin Blackadar, Jorg P. Kutter, Katayoun Saatchi, Urs O. Hafeli
Summary: Albumin microparticles, particularly macroaggregated albumin (MAA), are commonly used in pharmaceutical applications for drug delivery and imaging. A microfluidic platform was developed to create more tunable and precise MAA particles, demonstrating potential for pharmaceutical utility in targeted drug delivery and distribution assessment in vivo. The microfluidic chip offers an elegant and controllable method for preparing albumin microparticles for biomedical applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Chemistry, Analytical
Gerard Comamala, Camilla C. Krogh, Vibe S. Nielsen, Joerg P. Kutter, Josef Voglmeir, Kasper D. Rand
Summary: This study presents an improved method for HDX-MS analysis of challenging PTM-rich proteins by immobilizing enzymes on microfluidic chips to create IMERs. The combination of different enzymes allows for efficient online disulfide-bond reduction, deglycosylation, and proteolysis in HDX-MS analysis. This integrated workflow enables the mapping of epitopes and identification of regions involved in protein interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Zishuo Yuan, Drago Sticker, Hans Christian Cederberg Helms, Jorg P. Kutter
Summary: Proper oxygen supply is crucial for obtaining reliable results in in vitro cell models. This study employed microfluidic chips made from thiol-ene polymers to generate controlled oxygen gradients. Kinetic studies revealed that physisorbed water on channel surfaces and in the immediate bulk plays a significant role in the oxygen scavenging process.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Biochemical Research Methods
Yuye Zhou, Alexander Jonsson, Drago Sticker, Guojun Zhou, Zishuo Yuan, Jorg P. Kutter, Asa Emmer
Summary: This study utilized a microfluidic chip to analyze the functions and compositions of two important proteins, osteopontin and immunoglobulin G. The results showed that the microfluidic chip had the advantages of rapid and efficient protein digestion and glycopeptide enrichment, and it had potential applications in detecting protein biomarkers of related diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Caroline Despicht, Cecilie H. Munkboel, Hua Nee Chou, Peter Ertl, Mario Rothbauer, Jorg P. Kutter, Bjarne Styrishave, Andreas Kretschmann
Summary: The development of cell-based microfluidic assays offers new opportunities in toxicity testing through integration of new functionalities, automation, and high throughput. This study aimed to miniaturize the H295R steroidogenesis assay from the well-plate format to a microfluidic chip. PDMS and thiol-ene were tested as potential chip materials, and thiol-ene showed more favorable properties for H295R cell culture. A microfluidic chip design and cell seeding procedure were successfully developed, achieving repeatable and uniform cell distribution.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Junwei Wang, Jorg P. Kutter, Huiling Mu, Arshnee Moodley, Mingshi Yang
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Biochemical Research Methods
Reka Geczy, Drago Sticker, Nicolas Bovet, Urs O. Hafeli, Jorg P. Kutter
