Article
Chemistry, Analytical
Yang Li, Yi Yang, Yinghui Liu, Jinyuan Liu, Yunjia Yang, Jing Zhang, Yue Zou, Lin Shu, Nanyin Han, Bing Shao
Summary: A new method was developed for measuring and diagnosing total IgE in serum for food allergies. This method uses magnetic beads and ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to extract and quantify IgE, and combines in vitro binding experiments to evaluate its binding capacity with different allergenic proteins, thus speculating the allergen causing allergic reactions.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jiaojiao Sun, Haimei Shi, Yuyan Xue, Wenmin Cheng, Menghuan Yu, Chuanfan Ding, Fuxing Xu, Shaoning Yu
Summary: The accuracy of bacterial identification based on MALDI-TOF MS can be affected by functional proteins, releasing bacteria from functional material can increase accuracy.
Article
Nanoscience & Nanotechnology
Chenxu Wang, Xuefei Miao, Jiaju Zha, Wenhui Guo, Qingyong Ren, Yujing Zhang, Feng Xu, Luana Caron
Summary: The geometry of a magnetocaloric regenerator plays a crucial role in the performance and heat exchange efficiency of a magnetic refrigerator. A new and easy method has been proposed to produce monolithic MRs with highly ordered and unidirectional oriented microchannels. The MRs retain an orthorhombic perovskite structure after sintering, exhibiting a significantly higher isothermal entropy change.
SCRIPTA MATERIALIA
(2023)
Article
Cell & Tissue Engineering
Alessandro Blasimme, Jeremy Sugarman
Summary: Human pluripotent stem cells have the potential to create in vitro models that mimic post-implantation human embryos. However, ethical concerns arise from such integrated embryo models, and it is necessary to address them in order to establish appropriate policies and regulations that support scientific innovation and medical progress.
Article
Agriculture, Multidisciplinary
Xin Zhao, Jiayin Lu, Shuping Long, Winnie C. Soko, Qin Qin, Liang Qiao, Hongyan Bi
Summary: A strategy combining IMS and MALDI-TOF MS was developed to test seafood allergens, with consistent results compared to ELISA tests and a 0% false positive rate. The method shows potential for detecting seafood allergens to ensure the safety of allergic patients.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemical Research Methods
Chiara Lago, Matteo Gianesello, Lucia Santomaso, Gloria Leva, Claudio Ballabio, Marica Anderle, Francesco Antonica, Luca Tiberi
Summary: This article describes a protocol for generating medulloblastoma and high-grade glioma organoids by electroporating cerebellum and forebrain organoids, and provides detailed procedures for drug screening, lineage tracing, co-culture, and in vivo assays. Human organoids derived from genetically modified cerebral organoids differentiated from hiPSCs have been shown to be a valid alternative model for studying brain cancer biology, genetics, and testing therapies. The protocol includes the generation of cerebellum and forebrain organoids, genetic modification, orthotopic transplantation, co-culture experiments, lineage tracing, and drug screening. The entire process takes 60-65 days for cancer organoid generation and 1-4 weeks for downstream applications. It requires at least 3-6 months of proficiency in hiPSCs culture, organoid generation, and procedures on immunodeficient mice.
Article
Chemistry, Analytical
C. Toyos-Rodriguez, A. Llamedo-Gonzalez, D. Pando, S. Garcia, J. A. Garcia, F. J. Garcia-Alonso, A. De la Escosura-Muniz
Summary: Magnetic beads (MBs) are widely used in biosensing platforms due to their magnetic behavior, simplifying sample purification and separation. In this study, novel, reliable, and low-cost MBs were synthesized and used as electrochemical immunosensing platforms, showing improved performance.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Zhaoliang Chai, Hongyan Bi
Summary: In this study, anti-E. coli functionalized magnetic beads were constructed and successfully used to capture E. coli from fish sarcoplasmic protein and fish muscle protein extracts. The reproducibility and accuracy of the method were demonstrated through experiments. The method showed high specific detection of target bacteria in complex biological samples.
Article
Multidisciplinary Sciences
Angus McMullen, Sascha Hilgenfeldt, Jasna Brujic
Summary: This research demonstrates that by controlling the number of bonds between particles and selecting the appropriate molecular properties of linkers, programmed microscale self-organization can be achieved to form specific structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell & Tissue Engineering
Ayaka Yanagida, Daniel Spindlow, Jennifer Nichols, Anish Dattani, Austin Smith, Ge Guo
Summary: Researchers have developed a human embryo model called blastoid through self-organization, which can form blastocyst-like structures within a short period of time, displaying three tissue layers and mimicking natural blastocysts. Single-cell transcriptome analysis confirms the high fidelity of the blastoid model, providing a versatile and scalable experimental model for human embryo research.
Article
Cell Biology
Xulun Wu, Wentao Zhao, Hao Wu, Qiancheng Zhang, Yiming Wang, Kunyuan Yu, Jinglei Zhai, Fan Mo, Meijiao Wang, Shiwen Li, Xili Zhu, Xiaoyan Liang, Baoyang Hu, Guang-Hui Liu, Jun Wu, Hongmei Wang, Fan Guo, Leqian Yu
Summary: The interactions between extra-embryonic tissues and embryonic tissues are crucial for proper early embryo development. However, the understanding of this crosstalk is limited due to ethical restrictions, difficulty in obtaining natural human embryos, and lack of appropriate in vitro models. Our study showed that by aggregating human embryonic stem cells with human trophoblast stem cells, hESCs self-organized into an asymmetric structure with primitive streak-like cells exclusively located at one end and morphologically flattened cells presumed to be extra-embryonic mesoderm cells induced at the opposite end. This research revealed potential roles of the extra-embryonic trophectoderm in regulating proper primitive streak formation and inducing extra-embryonic mesoderm cells from the human epiblast.
CELL PROLIFERATION
(2023)
Article
Multidisciplinary Sciences
Tatsuya Fukuyama, Lucan Yan, Masahito Tanaka, Megumi Yamaoka, Kei Saito, Shih-Chieh Ti, Chung-Chi Liao, Kuo-Chiang Hsia, Yusuke T. Maeda, Yuta Shimamoto
Summary: The spindle is a dynamic intracellular structure essential for chromosome segregation during cell division. It can exhibit varied shape morphologies through nonrandom, bistable self-organization paths. The emergence of different spindle shapes is influenced by both physical and molecular factors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Saradha Venkatachalapathy, Dyuthi Sreekumar, Prasuna Ratna, G. Shivashankar
Summary: This study reveals the importance of actomyosin contractile forces in regulating heterogeneous cell-fate decisions. Mechanical checkpoints contribute to the heterogeneity in cell state transitions.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Parisa Fathi Rezaei, Shahab Ghanbari, Gholamreza Mahdavinia
Summary: The kappa carrageenan-chitosan nanomagnetics (CCMNs) and chitosan-pectin nanomagnetics (CPMNs) were synthesized and characterized. The exo-polygalacturonase (exo-PG) was purified and immobilized on CCMNs and CPMNs by physical adsorption. The immobilized Exo-PG on CPMNs and CCMNs beads showed retained activity after multiple cycles, indicating their potential application in food industries.
PROCESS BIOCHEMISTRY
(2023)
Article
Medicine, General & Internal
Dan Ma, Nana Liang, Liyun Zhang
Summary: There is no simple method for early diagnosis and evaluation of rheumatoid arthritis (RA). This study aimed to determine potential biomarkers and establish diagnostic patterns for RA using proteomic fingerprint technology combined with magnetic beads. The diagnostic model with four potential protein biomarkers showed high specificity and sensitivity in accurately distinguishing RA patients from healthy controls.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Analytical
Vera Dosedelova, Frantisek Foret, Martina Doubkova, Kristian Brat, Petr Kuban
Summary: Exhaled breath condensate (EBC) is a promising non-invasive sample for clinical diagnostics. A novel EBC collection device was developed to maintain a stable temperature during sampling process, which proved to significantly influence the concentration of metabolites in EBC at different sampling temperatures.
Article
Chemistry, Analytical
Marco Morani, Myriam Taverna, Zuzana Krupova, Lucile Alexandre, Pierre Defrenaix, Thanh Duc Mai
Summary: This study presents a novel microfluidic droplet-based strategy for high-performance isolation of extracellular vesicles (EVs) using a magnetic bead-based approach combined with polyethylene glycol (PEG) without the use of antibodies. The reproducibility of EV recovery was improved, and isolated EVs identity was confirmed using capillary electrophoresis (CE) coupled with laser-induced fluorescent (LIF) detection. A purpose-made droplet platform was developed to further improve EVs capture efficiency and high throughput, achieving a significant improvement in recovery rate while reducing sample and reagent volumes and operation time.
Article
Chemistry, Analytical
Theo Lienard-Mayor, Camille Bricteux, Amel Bendali, Nguyet-Thuy Tran, Arnaud Bruneel, Myriam Taverna, Thanh Duc Mai
Summary: In this study, a new microfluidic droplet platform called Lab-in-Droplet is presented for multistep glycoprotein sample treatment. The platform enables full automation, droplet sensing and heating, allowing for the realization of several advanced functions such as heating and mixing with organic solvents. The Lab-in-Droplet platform demonstrated superiority over the conventional batchwise protocol, with reduced reagent consumption, shorter processing time, improved glycan labeling yield, and maintained glycan separation profile.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Bin Yang, Thanh Duc Mai, Nguyet Thuy Tran, Myriam Taverna
Summary: This study presents a new integrated approach for fluorescent labeling and analysis of N-glycans using capillary electrophoresis. The approach utilizes the capillary as both a microreactor for labeling and a separation channel for analysis, allowing for automation and improved efficiency. The method was successfully applied to analyze fluorescently labeled N-linked oligosaccharides from human immunoglobulin G and rituximab.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Caroline Twarog, Elias Fattal, Magali Noiray, Brigitte Illel, David J. Brayden, Myriam Taverna, Herve Hillaireau
Summary: This study compares the biophysical interactions between exenatide and C-10 or SNAC, finding that specific supramolecular structures are formed and non-specific interactions dominate the binding process. Bile salts in the gastrointestinal lumen inhibit the interaction between these permeation enhancers and exenatide.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Editorial Material
Biochemical Research Methods
Lucile Alexandre, Jiashu Sun, Myriam Taverna, Wenwan Zhong
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Vladimir Jonas, Anna Tycova, Jan Prikryl, Adela Kotzianova, Vladimir Velebny, Frantisek Foret
Summary: In this study, silver nanoparticles were deposited on a highly hydrophilic nanofibrous composite using a nano spray for surface-enhanced Raman spectroscopy (SERS) mapping of a peptide-incorporated material. The distance between the nano spray and the sample was found to be the most crucial parameter, as it directly affected the moisture content of the deposited colloid. Residual water was identified as a sensitivity enhancer. Additionally, the addition of a sodium chloride solution to the colloid increased its ionic strength, leading to a more homogeneous profile of the deposit. After the deposition process, the treated sample was scanned using a SERS laser, and the collected Raman spectra were transformed into a distribution map of the peptide at a concentration of 5 mu g/g.
Article
Chemistry, Analytical
Frantisek Foret, Doo Soo Chung, Jana Lavicka, Jan Prikryl, Haengdo Lee, Iveta Drobnikova
Summary: The International Inter-disciplinary Conference of Chemical Analysis, APCE-CECE-ITP-IUPAC 2022, held in Siem Reap, Cambodia, brought together analytical chemists from around the world to exchange knowledge and start collaborations.
Article
Chemistry, Analytical
Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Lac Thuy Huu Nguyen, Stephanie Descroix, Thanh Duc Mai
Summary: This study introduces a new concept of on-line dual preconcentration stages for capillary electrophoresis (CE), allowing two different preconcentration approaches to be used in the same capillary. The first stage involves dynamic magneto-extraction of target analytes using circulating magnetic beads, while the second stage utilizes electrokinetic preconcentration to focus the eluted analytes into a nano band before CE separation. A purpose-made instrument combining electrophoretic and microfluidic modules is used to control the movement of magnetic beads and analyte flow. The potential of this new enrichment principle and instrument is demonstrated for CE separation of target double-stranded DNA (ds-DNA) using light-emitting-diode-induced fluorescent (LEDIF) detection. The dual-stage preconcentration allows for in-capillary sample pretreatment and CE separation without mismatch of working volumes or waste of pretreated samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Joanie Otin, N. Thuy Tran, Aurelie Benoit, Corinne Buisson, Myriam Taverna
Summary: This study proposes a capillary electrophoresis method to analyze misused growth hormone-releasing hormone (GHRH) analogs. The use of dimethyl-beta-cyclodextrin as a chiral selector allows the separation of basic peptide analogs, including enantiopeptides that differ by one amino acid chirality. Electrokinetic preconcentration methods were investigated to increase sensitivity. The combination of preconcentration with chiral capillary zone electrophoresis successfully separates GHRH analogs with detection limits ranging from 75 to 200 ng/mL.
Review
Chemistry, Analytical
Denisa Smolkova, Richard Cmelik, Jana Lavicka
Summary: Capillary electrophoresis is widely used for oligosaccharide and glycan analysis, but the low electrophoretic mobility and lack of chromophore or fluorophore in saccharide molecules require modification before separation and detection. This review summarizes the labeling strategies used for oligosaccharide/glycan analysis, categorized based on the labeling mechanism and saccharide molecule. In addition to the commonly used 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, less common and recently developed tags applicable in glycomics are also summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Natalia Melicherova, Vit Gloser, Jan Skalak, Martin Trtilek, Jana Lavicka, Frantisek Foret
Summary: The study developed an online sampling and CE analysis method for plant xylem sap to study plant fitness under stress conditions. The method allows for real-time monitoring of sap composition changes, which is beneficial for investigating stress-induced alterations.
Review
Chemistry, Analytical
Blanca H. Lapizco-Encinas, Y. Victoria Zhang, Putuma P. Gqamana, Jana Lavicka, Frantisek Foret
Summary: Capillary electrophoresis (CE) is a powerful separation technique that offers high resolution, great sensitivity, and short processing times. When combined with mass spectrometry (MS), CE-MS systems provide accurate characterization of ionized species and significantly enhance the capabilities of MS. This review article introduces the analytical potential of CE-MS systems and discusses recent advances and future developments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Denisa Smolkova, Dana Moravcova, Jana Lavicka
Summary: Sample preparation is crucial for the analysis of oligosaccharides and glycans. Introduction of a positive charge into saccharide molecule improves the analysis. This study evaluates the performance of five solid-phase extraction sorbents for clean-up and concentration of positively labeled maltooligosaccharides.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Trong Khoa Mai, Frederic Halgand, Thanh Duc Mai
Summary: A new method for studying the orientation and density of mouse antibody grafting on magnetic beads, which can be used in immunoassays and biosensors with fluorescent detection of biomolecules, is reported in this study. The method involves selective enzymatic digestion to obtain F(ab')2 and Fc fragments, followed by separation and determination of these fragments using size exclusion chromatography (SEC) coupled with fluorescence detection (FLD). The developed method was used to evaluate the grafting capacity of immunoglobulin (IgG2a) on different biofunctionalized magnetic beads, and the results show that tosyl-activated and protein G beads have superior grafting capacity compared to carboxylic beads at the optimal IgG:bead ratios. Under optimized conditions, more than 70% of antibodies were grafted onto tosyl-activated and protein G beads in the correct orientation. The method was then demonstrated with commercially available antibodies specific to amyloid-beta peptide 1-42 (A beta 1-42) for magneto-immunoassays and fluorescent detection of this peptide, which is an established biomarker for the molecular diagnosis of Alzheimer's disease.
