Article
Medical Laboratory Technology
Junko Fujihara, Yoshikazu Takinami, Yasuyuki Kawai, Kaori Kimura-Kataoka, Haruo Takeshita
Summary: The study found significantly higher serum cfDNA concentrations in postmortem subjects compared to healthy controls and patients with cardiac disease, especially in those who died from asphyxia. Fragment larger than 10,000 bp was rare in postmortem samples, while a high concentration of 150-200 bp fragments was characteristic of postmortem subjects.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Abdallah M. Zeid, Jenny Jeehan M. Nasr, Fathalla Belal, Mohamed Walash, Noritada Kaji, Yoshinobu Baba
Summary: A green chiral lab-on-a-chip cyclodextrin-based microchip electrophoretic method was developed for enantioseparation of BCN and Phe enantiomers. The method utilized the synergistic effects of cyclodextrin and methylcellulose, achieving efficient separation in short time.
MICROCHEMICAL JOURNAL
(2021)
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Muhammad Noman Hasan, Ran An, Asya Akkus, Derya Akkaynak, Adrienne R. Minerick, Chirag R. Kharangate, Umut A. Gurkan
Summary: Paper-based microchip electrophoresis shows potential for bringing lab tests to the point of need, but issues with high electrical values causing pH and temperature changes can impact the accuracy and reproducibility of tests. The development of HemeChip aims to provide low-cost, rapid, and accurate electrophoresis tests for hemoglobin analysis. Through pH and temperature characterization, and acid pretreatment of cellulose acetate paper, researchers were able to mitigate shifts and create a stable environment for reproducible hemoglobin electrophoresis separation on HemeChip.
Review
Chemistry, Analytical
Marija Jozanovic, Iva Pukles, Nikola Sakac, Emanuel Carrilho, Aniko Kilar, Brunislav Matasovic, Mirela Samardzic, Mateja Budetic, Ferenc Kilar
Summary: Microchip electrophoresis is the most promising method for integrated lab-on-a-chip devices, and the use of nanomaterials in electrophoresis has opened up vast possibilities. This comprehensive review provides an overview of recent applications of nanomaterials in microchip electrophoresis, including their use as stationary and pseudostationary phases, as well as for analyte preconcentration. The review aims to highlight the increased capabilities of microchip electrophoresis and nanomaterial synergy in analytical separation methods.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hongyu Nie, Zhihui Li, Xiaokun Wang, Rongmeng Gu, Hongyan Yuan, Yong Guo, Dan Xiao
Summary: In this study, a multi-input capacitively coupled contactless conductivity detector (MICD)-D-4 was proposed with high sensitivity, and further improved by combining it with differential detection to form (DFMICD)-D-4. The new detector showed improved performance compared to traditional single-input and double-input detectors, with a higher response-to-baseline ratio and a low limit of detection (0.7 nM). It can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis, demonstrating its versatility and superiority over standard conductivity meters.
Article
Biochemistry & Molecular Biology
Lili Chang, Dan Wang, Cunzhi Peng, Qi Wang, Bingqiang Xu, Zheng Tong
Summary: Separation of nucleic acids and proteins is crucial in molecular biology research. This study found that high-concentration agarose gels can be easily dissolved in an autoclave and have good resolution for DNA and protein bands. Improved buffer conditions also contributed to the success of high-concentration agarose gel electrophoresis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biotechnology & Applied Microbiology
Amadeo Sena-Torralba, Yulieth D. Banguera-Ordonez, Laia Mira-Pascual, Angel Maquieira, Sergi Morais
Summary: To decentralize health care, the development of point-of-care (PoC) assays has gained attention. The lateral flow immunoassay (LFIA) is seen as a promising bioanalytical method due to its low cost and single-step detection process. However, its limited sensitivity and inability to detect disease biomarkers at clinically relevant levels have limited its application. This review explores the potential of merging different electrokinetic phenomena into paper-based assays to enhance their analytical performance.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Polymer Science
Hiroyuki Ohshima
Summary: This study develops a general theory for the time-dependent transient electrophoresis of a weakly charged spherical colloidal particle in a polymer gel medium. The Laplace transform of the transient electrophoretic mobility is derived by considering the hydrodynamic interaction between the particle and the gel medium based on the Brinkman-Debye-Bueche model. The theory covers both transient gel electrophoresis and transient free-solution electrophoresis, with the relaxation time for the former being shorter and decreasing as the Brinkman screening length decreases.
Article
Polymer Science
Yi Yang, Xiaodong Li, Hao Jiang, Mengchen Ge, Xing Su, Meishuai Zou, Guoping Li
Summary: A novel strategy was demonstrated to construct functionalized carbon nanotubes (CNTs) with polyurethane (PU) chains by combining mussel-inspired dopamine chemistry and polycondensation reactions. The poly-dopamine (PDA) was first functionalized to the surface of CNTs, then acted as a bridge and covalently reacted with polyurethane chains to form a network structure called CNTs-PDA-PU, which enhanced the grafting ability of polyurethane chains onto CNTs. The functionalized carbon nanotubes significantly improved the mechanical and damping properties of nanocomposites.
Article
Chemistry, Analytical
Ping Sun, Jing Wu, Shenghong Yang, Hongli Li, Lei Zhao, Yuanhang Wang, Xiayan Wang, Qiaosheng Pu
Summary: By investigating microchips with sub-millimeter channels, this study successfully reduced cost and improved ease of operation and separation efficiency, making microchip capillary electrophoresis a viable alternative to conventional slab gel electrophoresis in most biological laboratories.
Article
Chemistry, Physical
Valentina Trovato, Eti Teblum, Yulia Kostikov, Andrea Pedrana, Valerio Re, Gilbert Daniel Nessim, Giuseppe Rosace
Summary: A novel method is proposed to turn insulating cotton fabrics into electrically conductive materials by depositing VACNT, with shorter VACNT-based composite showing the best electrical conductivity. The developed e-textiles are suitable for application as humidity sensing smart textiles, demonstrating adequate response time and repeatability for end-users.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemical Research Methods
Lindsay Schneider, Anubhav Tripathi
Summary: This research presents a new method for identifying and separating target DNA of the same size based on targeted sequences. The method allows for the evaluation of multiple target analytes in a sample without fluorescence detection. The method utilizes microfluidic electrophoresis to separate different 150 bp target sequences into different sizes. It has the potential to be used for mutation detection and multiplexed analysis.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Tonoy K. Mondal, J. Hunter West, Stuart J. Williams
Summary: By using conductive carbon nanofiber mats, large-scale trapping of nanoparticles can be achieved to overcome Brownian transport. This method is cost-effective compared to traditional cleanroom procedures and can serve as a foundation for microscale electrokinetic devices.
Article
Chemistry, Multidisciplinary
Wenhao Yang, Wangyi Zeng, Liang Chai, Yanxin Jiang, Longjiang Deng, Guang Yang
Summary: In this work, a multifunctional conductive fabric was successfully prepared and demonstrated excellent performance as a corrosion resistant, durable, and lightweight strain sensor for modern flexible wearable devices. The fabric showed high sensitivity, wide detection range, excellent stability and durability, superhydrophobicity, and efficient photothermal conversion performance.