期刊
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
卷 12, 期 2, 页码 -出版社
UNIV CATOLICA DE VALPARAISO
DOI: 10.2225/vol12-issue2-fulltext-12
关键词
metal ions; nitrate reductase; nitrite accumulation; nitrite reductase; Paracoccus pantotrophus
资金
- The Royal Golden Jubilee (RGJ) [2.B.WL/45/A.1]
The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accumulation. Enzymatic studies revealed that the membrane-bound nitrate reductase and the periplasmic nitrite reductase had activities of 998 +/- 28 and 373 +/- 18 nmol (mg protein)(-1) min(-1), respectively after growing Paracoccus pantotrophus P16 in medium supplemented with 1.5 mu M Fe3+. If provided with 1.5 mu M Fe3+ and 2.4 mu M Mo6+, the membrane-bound nitrate reductase activity increased to 6,223 +/- 502 nmol (mg protein)(-1) min(-1) and the periplasmic nitrite reductase was 344 +/- 20 nmol ( mg protein)(-1) min(-1). The results indicated that an addition of Fe3+ and Mo6+ led to an overstimulation of nitrate reductase activity as compared with nitrite reductase activity. When glucose was supplied, the minimal ratio of carbon per nitrate (C/N) was 2.31 mg C/mg NO3--N with denitrification yield of 0.45 g NO3--N/g C. Addition of ethanol instead of glucose, the minimal ratio of C/N was 1.15 mg C/mg NO3--N with denitrification yield of 1.08 g NO3--N/g C.
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