期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 48, 期 -, 页码 28-31出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2014.08.007
关键词
L-DOPA; Serum; Screen printed electrodes; Carbon nanotubes; Tyrosinase; Thionine
A disposable electrochemical biosensor for the determination of L-DOPA in undiluted human serum is described. It is based on the use of a carbon nanotubes-modified screen printed electrode covered with an electropolymerized poly(thionine) film and an outer cross-linked layer containing tyrosinase. Further coverage of the sensor with a Nafion layer allows its use directly in human serum, obviating the need for sample pretreatment. The sensor exhibited a linear behavior in the 0.8-22 mu M concentration range and offers promise for decentralized clinical testing of L-DOPA. (C) 2014 Elsevier B.V. All rights reserved.
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