期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 18, 期 -, 页码 81-84出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2012.02.024
关键词
Cytosensor; HeLa cell; DPV; Surface-confined redox probe
资金
- National Natural Science Foundation of China [11174105, 21075120, 21105095]
- 973 project [2009CB930100, 2010CB933600]
The possibility of using electrochemical methods to investigate cell immobilization and natural physiological states provides great advances in life science research and public health protection. Herein, cytosensors with surface-confined ferrocene as signal indicator were developed to study the immobilization of human cervical carcinoma (HeLa) cells. With layer-by-layer (LBL) technique positively charged ferrocene functionalized poly(allylamine hydrochloride) (Fc-PAH) and negatively charged single-wall carbon nanotubes (SWNTs) were alternately assembled on 3-mercaptopropionic acid (MPA) modified gold substrates. The as-prepared cytosensors presented good biocompatibility and HeLa cells could keep viability for 72 h on the materials according to the proliferation results. With differential pulse voltammetry (DPV) measurements the cytosensors exhibited high sensitivity to the detection of HeLa cells within a wide concentration range from 10 to 10(7) cells/mL. (C) 2012 Elsevier B.V. All rights reserved.
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