期刊
ELECTROANALYSIS
卷 23, 期 5, 页码 1168-1174出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000595
关键词
Scanning electrochemical microscopy; Nanoelectrodes; Single cell analysis
资金
- MEXT (Ministry of Education, Culture, Sports, Science and Technology), Japan [18101006, 9750055, 22245011]
- Center for Interdisciplinary Research, Tohoku University
- Grants-in-Aid for Scientific Research [22245011] Funding Source: KAKEN
The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in mu m-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.
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