期刊
ELECTROANALYSIS
卷 23, 期 1, 页码 91-99出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201000486
关键词
PSA; Tumour marker; Electrochemical detection; Microfluidic; Magnetic beads; Multiarray
In this paper, two simple and sensitive approaches for prostate specific antigen (PSA) detection are presented, by using antibody modified paramagnetic microparticles coupled to multiplexed electrochemical platforms. The first proposed approach is based on 8-sensors screen-printed arrays as candidate for electrochemical transducers and a simple target capturing step by means of antibody-functionalised magnetic beads. In order to improve the performances of the immunosensing, a second approach is developed by using GRAVI-Cell. This is microfluidic-based affinity assay device for running bead-protocols. This innovative system combines a special chip containing eight polymer microchannels, with a portable, computer-controlled instrument. Both immunosensing strategies developed promise to be a sensitive, multiplexed tool for fast and easy PSA analysis.
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