High Performance Oxide Functionalized Nitrogen-Doped Mesocellular Carbon Foam for Biosensor Construction

Nitrogen-doped mesocellular carbon foam (denoted as MCF-CNx) with high surface area and large pore volatile was prepared and characterized in detail. The MCF-CNx was further functionalized by oxidation with HNO3 (denoted as MCF-CNx-O) in order to effectively improve its hydrophilicity and biocompatibility. Both MCF-CNx and MCF-CNx-O were used for immobilization of Hb and design of electrochemical biosensors. The activity of Hb immobilized on MCF-CNx-O is two times higher than that of Hb immobilized oil MCF-CNx. The Hb-MCF-CNx-O-Nafion modified electrode displays fast response. high sensitivity and low detection limit to the detection of hydrogen peroxide. The sensitivity of Hb-MCF-CNx-O-Nafion modified electrode (477 mu A mM(-1) cm(-2)) is twice that of Hb-MCF-CNx-Nafion modified electrode.