期刊
ELECTROANALYSIS
卷 21, 期 20, 页码 2190-2197出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200904681
关键词
DNA hydridization; SERS; Electrochemical melting curve; Anthraquinone label; Base pair mismatches
资金
- ORSAS
In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting (E-melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22-mer oligonucleotides labelled with a modified anthraquinone at the 3'-end. Discrimination between the wild type, 1653C/T (point mutation) and Delta F508 (triple deletion) is demonstrated.
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