期刊
ELECTROANALYSIS
卷 21, 期 16, 页码 1862-1868出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200904647
关键词
Biosensors; Prussian blue; Glucose; Hydrogen peroxide; Ionic liquids
资金
- Institute for Advanced Studies in Basic Science (IASBS)
Prussian blue modified carbon ionic liquid electrodes (PB-CILEs) were fabricated using chemical and electrochemical procedures. Chemically fabricated PB-CILE exhibited an excellent sensitivity (0.0866 mu A mu M(-1)), low detection limit (0.01 mu M) and two linear ranges (0.01-1 and 1-600 mu M) toward hydrogen peroxide. Then, glucose oxidase (GOx) was immobilized on the surface of PB-CILE to fabricate glucose biosensor using three different procedures involving cross linking with glutaraldehyde (GLU) and bovine serum albumin (BSA), entrapment into the Nafion matrix and covering with a sol-gel layer. Glucose biosensor fabricated using cross linking procedure showed the best sensitivity (0.0019 mu A mu M(-1)) and operational stability for glucose.
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