Article
Engineering, Environmental
Yunhan Jiang, Ying Liu, Jian Zhang
Summary: Mixed antibiotics can stimulate the growth and microcystin synthesis of Microcystis aeruginosa by activating photosynthesis, carbon metabolism, and other pathways to enhance resistance to oxidative stress. This study provides insights into the mechanisms underlying the hormetic stimulation of harmful cyanobacterial blooms by antibiotic contaminants.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Xiaodi Duan, Chiqian Zhang, Ian Struewing, Xiang Li, Joel Allen, Jingrang Lu
Summary: This study evaluated the efficacy of an early-warning system that uses cyanotoxin-encoding genes to predict harmful cyanobacterial blooms in Harsha Lake, Ohio. The results showed that the abundances and transcript levels of cyanotoxin-encoding genes were significantly correlated with total cyanotoxin concentrations and accurately predicted cyanotoxin production. This early-warning system can be a useful tool for lake managers in predicting and preventing harmful cyanobacterial blooms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Marine & Freshwater Biology
Li Kang, Maira Mucci, Miquel Lurling
Summary: In this study, nine products were tested for their effectiveness in combating harmful cyanobacterial blooms in lakes. Algicides, chitosan, and hydrogen peroxide were found to be the most powerful in reducing cyanobacteria biomass and impacting photosynthetic efficiency and phosphorus concentrations.
Article
Environmental Sciences
Gabriela Rocio Sosa Benegas, Suzan Prado Fernandes Bernal, Valeria Maia de Oliveira, Michel Rodrigo Zambrano Passarini
Summary: The study revealed the potential of Antarctic bacteria to inhibit the proliferation of Microcystis aeruginosa and degrade microcystin-LR, suggesting their possible application in mitigating cyanobacterial blooms in the future as an alternative or supporting approach.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Engineering, Environmental
Tesfaye Muluye, Tadesse Fetahi, Flipos Engdaw, Adem Mohammed
Summary: Public concerns about the production of cyanotoxins in water and its harmful effects on human and animal health are increasing due to eutrophication in aquatic ecosystems. A review of 64 studies from 1990 to 2022 found cyanotoxins in 95 waterbodies across 15 African countries, with microcystins being the most common toxin. Concentrations of microcystins exceeding the WHO drinking water guideline were reported in 63% of the surveyed aquatic ecosystems. Monitoring of nutrients, cyanobacteria, and cyanotoxins in African waterbodies is necessary for sustainable water resources management.
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
(2023)
Article
Environmental Sciences
Giridhar Babu Anam, Dinneswara Reddy Guda, Young-Ho Ahn
Summary: The study investigated the response of Microcystis aeruginosa to phytohormone exposure under terpinolene stress. Results showed that terpinolene inhibited growth and photosynthetic activities of M. aeruginosa while stimulating microcystins production through oxidative stress and antioxidant defense mechanisms. Hormones like IAA and BSL played critical roles in enhancing antioxidant defense mechanisms and microcystins production under terpinolene stress.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Mauro Cesar Palmeira Vilar, Thiago Ferreira da Costa Pena Rodrigues, Aloysio da Silva Ferrao-Filho, Sandra Maria Feliciano de Oliveira e Azevedo
Summary: Cyanobacteria, as photosynthetic microorganisms, play a crucial role in energy flow within aquatic food webs by interacting with zooplankton. Despite their ability to produce toxic metabolites known as cyanotoxins, the ecological and adaptive role of these toxins remains debated. Studies have suggested that cyanotoxins may serve as a defense mechanism against herbivory by zooplankton, but their overall impact on cyanobacteria fitness is still unclear.
JOURNAL OF CHEMICAL ECOLOGY
(2021)
Article
Microbiology
Liang Zhao, Li-Zhou Lin, Ying Zeng, Wen-Kai Teng, Meng-Yun Chen, Jerry J. Brand, Ling-Ling Zheng, Nan-Qin Gan, Yong-Hui Gong, Xin-Yi Li, Jin Lv, Ting Chen, Bo-Ping Han, Li-Rong Song, Wen-Sheng Shu
Summary: In freshwater ecosystems, the interaction between algae and heterotrophic bacteria may lead to the formation of bacterial populations with the ability to degrade phosphonates, increasing the proliferation capabilities of cyanobacteria. Metatranscriptomic analysis of aggregate samples suggests that the cooperation between algae and heterotrophic bacteria plays a crucial role in the proliferation of cyanobacteria.
Article
Food Science & Technology
Jingjing Zhou, Mingzhi Qu, Christy Dunkinson, Daniel D. Lefebvre, Yuxiang Wang, R. Stephen Brown
Summary: The presence of cyanobacteria during a harmful algal bloom can significantly affect the survival of Escherichia coli bacteria. This is due to the toxic effect of cyanobacteria-produced microcystins on E. coli. Therefore, the use of E. coli as a fecal indicator bacteria needs to take into consideration the presence of cyanobacteria.
Article
Biotechnology & Applied Microbiology
Micheline Kezia Cordeiro-Araujo, Adriana Sturion Lorenzi, Mathias Ahii Chia, Eliane Christina Mota, Maria do Carmo Bittencourt-Oliveira
Summary: This study investigated the combined effects of light intensity, UV-B radiation, and temperature on the circadian rhythm of toxin production, gene expression, and H2O2 formation in Microcystis aeruginosa. The results showed that different conditions of light and UV-B radiation altered the circadian rhythm of microcystin production and release, impacting gene expression.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Food Science & Technology
He Zhang, Yan Xie, Rongzhen Zhang, Zhongliang Zhang, Xinglong Hu, Yao Cheng, Ruozhen Geng, Zengling Ma, Renhui Li
Summary: Harmful cyanobacterial blooms pose a threat to aquatic ecosystems and public health globally. Searching for high efficiency algicidal bacteria has become an important task. In this study, a bacterial strain named Streptomyces sp. HY exhibited highly algicidal activity against Microcystis aeruginosa and other cyanobacterial strains, while showing minimal impact on green algae. The algicidal mechanism involved damages to the photosynthesis system, morphological injury of algal cells, oxidative stress, and dysfunction of the DNA repair system. HY treatment also reduced the expression levels of genes related to microcystin biosynthesis. Overall, Streptomyces sp. HY shows promise as a candidate for controlling harmful cyanobacterial blooms.
Article
Food Science & Technology
Viktor A. Gabyshev, Sergey I. Sidelev, Ekaterina N. Chernova, Anna A. Vilnet, Denis A. Davydov, Sophia Barinova, Olga I. Gabysheva, Zoya A. Zhakovskaya, Ivan V. Voronov
Summary: This study tested the hypothesis of year-round presence of toxigenic Microcystis and cyanotoxins in Lake Ytyk-Kyuyol. Three independent approaches were used: mass-spectrometry, molecular methods, and light microscopy. Microcystis flos-aquae and M. aeruginosa were the dominant species, and the presence of M. aeruginosa and microcystin biosynthesis genes were confirmed. Microcystin concentration was low, indicating the potential viability of overwintering colonies.
Article
Geosciences, Multidisciplinary
Valeria M. Lemos, Marianna Lanari, Margareth Copertino, Eduardo R. Secchi, Paulo Cesar O. de Abreu, Jose H. Muelbert, Alexandre M. Garcia, Felipe C. Dumont, Erik Muxagata, Joao P. Vieira, Andre Colling, Clarisse Odebrecht
Summary: Estuaries are important ecosystems that provide ecological and economic services, but they are facing threats worldwide. Long-term ecological studies are crucial for understanding and managing estuarine functioning, and for predicting scenarios.
EARTH SYSTEM SCIENCE DATA
(2022)
Article
Engineering, Environmental
Yixin He, Guining Wei, Bingran Tang, Muhammad Salam, Ai Shen, Yanyan Wei, Xin Zhou, Mengzi Liu, Yongchuan Yang, Hong Li, Yufeng Mao
Summary: This study investigates the interactions between microplastics (MPs), toxins, and biofilm in water environments. The results show that MPs can adsorb toxic substances and be colonized by microorganisms, leading to the reduction of toxins in water. The colonization of microorganisms on MPs enhances the bio-adsorption of toxins, while certain bacteria in the biofilm facilitate the biodegradation of toxins. These findings provide new insights into the ecological risks of microplastics.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Ronald B. Souza, Margareth S. Copertino, Gilberto Fisch, Marcelo F. Santini, Walter H. D. Pinaya, Fabiane M. Furlan, Rita de Cassia M. Alves, Osmar O. Moller, Luciano P. Pezzi
Summary: This study measured the atmospheric CO2 concentrations in a salt marsh in southern Brazil and analyzed the CO2 exchange fluxes between vegetation and the atmosphere using eddy correlation techniques. The results showed that the CO2 fluxes were modulated by transient atmospheric systems and surrounding water level variations. It was found that the salt marsh acted as a CO2 sink during daytime and a CO2 source during nighttime. The seasonal changes in CO2 fluxes were influenced by sunlight, temperature, marsh inundation, winds, and plant biomass.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Simone Simionatto, Silvana Beutinger Marchioro, Marcelo dos Santos Barbosa, Vanessa Galli, Clarice Brink Brum, Sergio Jorge, Odir Antonio Dellagostin
Summary: This study standardized an ELISA method using six recombinant proteins of Mycoplasma hyopneumoniae for detection of serum antibodies in pigs, showing promising results in both diagnostic tests and seroprevalence surveys in commercial pig herds. The recombinant proteins P95 and P102 were identified as potential targets for future diagnostic tests in differentiating infected animals from vaccinated ones.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Everton B. Bettin, Jessica Dorneles, Amanda S. Hecktheuer, Andriele B. Madruga, Amilton C. P. Seixas Neto, Alan J. A. McBride, Thais L. Oliveira, Andre A. Grassmann, Odir A. Dellagostin
Summary: Leptospirosis is an emerging infectious disease caused by pathogenic Leptospira spp. Developing a universal vaccine requires identifying highly conserved epitopes exposed on the bacterial surface. A recombinant BCG vaccine targeting leptospiral TBDRs induced a protective and sterilizing immune response in Syrian hamsters, showing promise for preventing leptospirosis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Violetta Dias Pacce, Margarida Neves Souza, Natasha Rodrigues de Oliveira, Frederico Schmitt Kremer, Sergio Jorge, Nilo Ikuta, Vagner Ricardo Lunge, Odir Antonio Dellagostin
Summary: PCR and LAMP assays targeting novel genes lic13162 and lic20239 were developed to directly detect pathogenic Leptospira. The PCR assays were successful, while the LAMP assays had limitations.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Electrochemistry
Ana Luiza Lorenzen, Ariane Moraes dos Santos, Luani Poll dos Santos, Luciano da Silva Pinto, Fabricio Rochedo Conceicao, Franciele Wolfart
Summary: Electrochemical sensors and biosensors are useful for fast, inexpensive, sensitive, and easy detection of various specimens. In this study, a modified electrode was used to detect COVID-19 antibodies, showing a linear selective response. The method has the potential to replace traditional tests due to specific antibody-antigen binding interaction and fast response.
ELECTROCHIMICA ACTA
(2022)
Article
Immunology
Reginaldo G. Bastos, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M. Cooke, Odir A. Dellagostin, Raul G. Barletta, Carlos E. Suarez
Summary: This article discusses the potential use of BCG TI as a strategy to control babesiosis infection more efficiently, supported by experimental evidence and the possibility of vaccine development.
Article
Medicine, Legal
Gabriel O. Urtiaga, William B. Domingues, Eliza R. Komninou, Amanda W. S. Martins, Eduardo B. Blodorn, Eduardo N. Dellagostin, Rafael dos S. Woloski, Luciano S. Pinto, Clarice B. Brum, Luciana Tovo-Rodrigues, Vinicius F. Campos
Summary: SNP analyses from a forensic intelligence perspective have proven to be an important tool for limiting the number of suspected offenders and finding missing persons. This study used DNA microarray assay to analyze saliva samples collected from a glass surface using a double swab technique commonly applied at crime scenes. Results showed that reliable genotypes with a confidence level of more than 90% can be obtained with a Call Rate above 69.41%, regardless of the experimental condition. The study suggests that DNA microarray can support criminal investigation teams from a forensic intelligence perspective, and future improvements may eliminate the purification step in crime analysis and investigation.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Julio Henrique Ferreira de Sa Queiroz, Marcelo dos Santos Barbosa, Lais Goncalves Ortolani Miranda, Natasha Rodrigues de Oliveira, Odir Antonio Dellagostin, Silvana Beutinger Marchioro, Simone Simionatto
Summary: The study aimed to select and evaluate three Treponema pallidum recombinant proteins for syphilis serodiagnosis, and found that rTp0684 showed better potential as an antigen for the development of syphilis serodiagnosis.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Food Science & Technology
Maria Isabel Ferreira Campos, Paula Perazzo de Souza Barbosa, Laura Junqueira Camargo, Luciano Da Silva Pinto, Binaca Mataribu, Catarina Serrao, Luis Fernando Marques-Santos, Jose Honorio Lopes, Julia Mariano Caju de Oliveira, Carlos Alberto de Almeida Gadelha, Tatiane Santi-Gadelha
Summary: This study aimed to evaluate the biological activities and acute toxicity of goat whey proteins. The results showed that goat whey proteins exhibited antibacterial, antiproliferative, and antioxidant activities, and did not have harmful effects on Artemia franciscana larvae. Therefore, goat whey has great potential to be developed as a safe biopreservative or functional ingredient in the food industry.
Review
Immunology
Odir Antonio Dellagostin, Sibele Borsuk, Thais Larre Oliveira, Fabiana Kommling Seixas
Summary: This review explores the strategies for developing and using auxotrophic BCG strains, which can serve as a model for studying M. tuberculosis and potentially provide a stable and effective alternative to BCG, as well as a vector for recombinant live vaccines.
Article
Pharmacology & Pharmacy
Gabriela Haedrich, Gustavo Richter Vaz, Juliana Bidone, Virginia Campello Yurgel, Helder Ferreira Teixeira, Alexandre Goncalves Dal Bo, Luciano da Silva Pinto, Mariana Appel Hort, Daniela Fernandes Ramos, Antonio Sergio Varela Junior, Pedro Eduardo Almeida da Silva, Cristiana Lima Dora
Summary: This study aimed to produce nanostructured lipid carriers with novel glycosylated amphiphilic to attach WGA on the surface for improved intracellular drug delivery. The functionalization of nanocarriers with WGA protein increased phagocytosis by macrophages, showing potential for treating intracellular pathogens.
Article
Biotechnology & Applied Microbiology
Jenifer Malheiros Goncalves, Thayna Laner Cardoso, Stella Buchhorn de Freitas, Rafael Woloski, Amilton Clair Pinto Seixas Neto, Luciano da Silva Pinto, Elba Sampaio de Lemos, Daiane Drawanz Hartwig
Summary: Bartonella henselae, a Gram-negative bacterium, causes cat scratch disease (CSD) in humans through scratches or bites, with vaccination and efficient diagnostic methods being potential control measures. Recombinant chimeric proteins (rC1, rC2, rC3) were designed and evaluated for CSD control, with significant IgG antibody responses in mice immunized with rC1 and rC3 from day 28, and with rC2 from day 35.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Laura Junqueira de Camargo, Mara Andrade Colares Maia, Rafael dos Santos Woloski, Caroline Rizzi, Gustavo Marcal Schmidt Garcia Moreira, Claus Troger Pich, Luciano da Silva Pinto
Summary: A novel lectin, rBTL, was generated by modifying the amino acid sequence of BanLec. It maintained the structure and specificity of the carbohydrate-binding site, exhibited biological activity, and showed cytotoxicity against human melanoma cells.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Neurosciences
Thiago Angelo Smaniotto, Angela Maria Casaril, Darling de Andrade Lourenco, Fernanda S. Sousa, Fabiana K. Seixas, Tiago Collares, Rafael Woloski, Luciano da Silva Pinto, Diego Alves, Lucielli Savegnago
Summary: Our study investigated the effects of intranasal administration of interleukin-4 (IL-4) on mice with depressive-like behavior induced by chronic unpredictable mild stress (CUMS). We found that IL-4 reversed the depressive behavior and regulated oxidative stress markers in the brain structures, suggesting its potential as an innovative strategy for major depression (MD) treatment.
PSYCHOPHARMACOLOGY
(2023)
Article
Veterinary Sciences
Janaina Fadrique da Silva, Diego Alexandre Hemb Alba, Sergio Jorge, Patricia Gindri, Tatiane Senna Bialves, Guilherme Nunes de Souza, Fabio Raphael Pascoti Bruhn, Ligia Margareth Cantarelli Pegoraro, Odir Antonio Dellagostin
Summary: Through a study on dairy herds from different mesoregions of Rio Grande do Sul State in Southern Brazil, it was found that the prevalence of Leptospira antibodies was higher than expected, with Djasiman serogroup being particularly common, despite not being included in current commercial vaccines. The presence of wetlands and rodents as risk factors associated with Djasiman is consistent with scientific literature.
ACTA SCIENTIAE VETERINARIAE
(2022)
Article
Chemistry, Physical
Marcely Echeverria Oliveira, Bruno Vasconcellos Lopes, Jessica Helisa Hautrive Rossato, Guilherme Kurz Maron, Betty Braga Gallo, Andrei Borges La Rosa, Raphael Dorneles Caldeira Balboni, Mariliana Luiza Ferreira Alves, Marcos Roberto Alves Ferreira, Luciano da Silva Pinto, Fabricio Rochedo Conceicao, Evandro Piva, Claudio Martin Pereira de Pereira, Marcia Tsuyama Escote, Neftali Lenin Villarreal Carreno
Summary: A diagnostic chip platform based on a disposable electrochemical biosensor has been developed for the detection of SARS-CoV-2 antibodies. It helps with epidemiological public safety measures and the control of population's immune response to vaccines.
