Article
Multidisciplinary Sciences
Tomokazu Kurita, Masako Iwai, Keishi Moroi, Kumiko Okazaki, Seiji Nomura, Fumihiko Saito, Shinichiro Maeda, Akihide Takami, Atsushi Sakamoto, Hiroyuki Ohta, Tetsushi Sakuma, Takashi Yamamoto
Summary: Algal lipids, produced through photosynthesis and carbon dioxide assimilation, are expected to be the basis for sustainable fuels. However, the use of genetically modified organisms (GMOs) in outdoor culture is restricted. Recent research has discovered that plasmids containing yeast centromere and autonomous replication sequence can behave as episomes in Nannochloropsis species. This study aims to develop a genome editing system that allows the removal of expression vectors after introducing mutations, thereby creating mutated Nannochloropsis without any transgenes.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Maria Belenioti, Eirini Mathioudaki, Evrykleia Spyridaki, Demetrios Ghanotakis, Nikolaos Chaniotakis
Summary: Grape pomace is a significant issue in the Greek wine industry due to its negative environmental impact. However, by using Chlamydomonas reinhardtii, the polyphenols in grape pomace can be efficiently reduced, and the resulting product has potential as a soil conditioner.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Theocharis T. Nazos, Demetrios F. Ghanotakis
Summary: Immobilized Chlamydomonas reinhardtii cells in high concentrations of alginate beads exhibited higher metabolic efficiency, while lower alginate concentrations resulted in increased cell leakage.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Shun Tamaki, Kazunari Ozasa, Toshihisa Nomura, Marumi Ishikawa, Koji Yamada, Kengo Suzuki, Keiichi Mochida
Summary: Zeaxanthin is required for the formation of functional photoreactive organelles in Euglena gracilis, highlighting the evolutionary differences between euglenophytes and chlorophytes in the photosensing mechanism. The study demonstrates the metabolic regulation of photoreactive organelle formation in Euglena gracilis and its importance in eyespot formation.
Article
Engineering, Environmental
Neng Yan, Wen-Xiong Wang
Summary: This study demonstrates the internalization of silver nanoparticles (AgNPs) in unicellular algae, with different surface coatings affecting the pathways for cellular entry. Furthermore, trophic transfer of AgNPs from algae to zooplankton species was visualized, revealing differences in retention and distribution patterns based on surface coatings.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Plant Sciences
Yaqi An, Dong Wang, Jingxia Du, Xinwei Wang, Jianwei Xiao
Summary: The carbon dioxide emitted by human accounts for only a small fraction of global photosynthesis consumption, half of which is due to microalgae. The high efficiency of algae photosynthesis is attributed to the pyrenoid-based CO2-concentrating mechanism (CCM). The formation of pyrenoid which has a variety of Rubisco-binding proteins mainly depends on liquid-liquid phase separation (LLPS) of Rubisco, a CO2 fixing enzyme. The current research on the structure, assembly and application of Chlamydomonas reinhardtii pyrenoids is summarized in this article, providing new ideas for improving crop photosynthetic performance and yield.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Engineering, Environmental
Vera Slaveykova, Sanghamitra Majumdar, Nicole Regier, Weiwei Li, Arturo A. Keller
Summary: Metabolomics was used to study the response of green alga to sublethal concentrations of inorganic mercury and monomethylmercury. The alga adjusted its metabolism by increasing levels of various metabolites involved in different pathways after exposure to low concentrations of mercury compounds. Exposure to inorganic mercury resulted in more pronounced metabolic perturbations in comparison to monomethylmercury, while most metabolic disturbances were common between the two treatments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Environmental Sciences
Aoxue Sun, Limei Xu, Gaoxiang Zhou, Erqin Yin, Tiantian Chen, Yong Wang, Xiaochen Li
Summary: This study investigated the impacts of micro-plastics as carriers on the toxicity of heavy metals. It found that nano-sized plastics showed higher adsorption capacities and lower desorption rates for Pb2+ compared to micro-sized plastics. The presence of micro-plastics decreased the bioavailability of Pb2+ to Chlamydomonas reinhardtii and suppressed membrane damage and oxidative stress. The study also observed that the size of micro-plastics influenced their capacity to carry heavy metal ions and their toxicity to C. reinhardtii.
Article
Environmental Sciences
Zhongquan Jiang, Yutong Sun, Huize Guan, Danqing Sun, Shu Fang, Xuening Ma, Zhongyang Wang, Zhen Li, Chunhua Zhang, Ying Ge
Summary: The study found that when exposed to low concentrations of As(V), Chlamydomonas reinhardtii accumulated starch and produced more extracellular polysaccharides, while under high concentrations of As(V), the cells redirected carbon flux and transformed lipids into polysaccharides, indicating the critical involvement of polysaccharides in As(V) resistance in C. reinhardtii.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Shin-Ichiro Ozawa, Felix Buchert, Ruby Reuys, Michael Hippler, Yuichiro Takahashi
Summary: The substitution of P171L in the Rieske FeS PETC subunit of cytochrome b(6)f alters the pH dependency of the photosynthetic control mechanism. This study demonstrates that different stringency of photosynthetic control is established in Chlamydomonas reinhardtii depending on cellular metabolism, and the lumen pH-sensitive PETC-P171L mutant can be used to read out various associated effects. The Delta pH component in PETC-P171L depends on the availability of oxygen.
Article
Environmental Sciences
Fangbing Qi, Yu Gao, Jiaqi Liu, Xiangyu Yao, Kai Han, Ziyi Wu, Yingjuan Wang
Summary: Cadmium (Cd) pollution is a threatening challenge to the environment and human health, with serious impacts on natural organisms. Green algae, such as Chlamydomonas reinhardtii (C. reinhardtii), have the potential to be a safer and more cost-effective approach for treating heavy metal ions in wastewater due to their sorption properties. However, C. reinhardtii is affected by heavy metal ions when sorbed. Melatonin can protect plants from damage under stress conditions. In this study, the effects of melatonin on C. reinhardtii under Cd stress were investigated, including cell morphology, chlorophyll content, enzymatic activity, gene expression, and the AsA-GSH cycle. The presence of melatonin effectively protected the activity of photosynthetic system II, enhanced antioxidant activity, upregulated gene expression in the AsA-GSH cycle, and reduced the level of ROS, alleviating the damage caused by Cd toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Plant Sciences
Xiaosong Gu, Li Cao, Xiaoying Wu, Yanhua Li, Qiang Hu, Danxiang Han
Summary: This study identified galactosyl hydrolases (CrGH) in lipid bodies of Chlamydomonas reinhardtii and found that deficiency of CrGH led to an increase in MGDG and DGDG content and a decrease in TAG content under high light stress conditions. The up-regulation of CrGH under high light stress suggests its involvement in the conversion of photosynthetic membranes to TAGs, revealing a new paradigm for lipid metabolism in microalgae.
Article
Biotechnology & Applied Microbiology
Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
Summary: In this study, the accessibility of different isoprenoid precursor pools for sesquiterpenoid production in Chlamydomonas reinhardtii was investigated. It was found that both the cytoplasm and chloroplast can be utilized for the generation of sesquiterpenoids. Targeting the fusion protein to the chloroplast and increasing gene dosage significantly improved sesquiterpene production. In addition, potential prenyl unit transporters were proposed based on bioinformatic analyses, which could enhance the capabilities of sesquiterpenoid production.
MICROBIAL CELL FACTORIES
(2022)
Article
Biology
Karl F. Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, Pinfen Yang
Summary: Intraflagellar transport (IFT) plays a crucial role in protein transportation into flagella, but the interaction between IFT trains and diverse proteins remains largely unknown. This study reveals that ARMC2/PF27 is necessary for IFT of radial spokes in Chlamydomonas, providing insights into the assembly process of flagella.
Article
Biochemistry & Molecular Biology
Ashish K. Maurya, Piali Sengupta
Summary: The paper introduces a novel cilia regulator called XBX-4, which modulates the CCRK and MAK kinase pathway in C. elegans. Mutations associated with Joubert syndrome in the XBX-4 DUF3719 domain also result in cilia elongation.