Article
Biochemistry & Molecular Biology
Xukai Jiang, Yuliang Sun, Kai Yang, Bing Yuan, Tony Velkov, Lushan Wang, Jian Li
Summary: The study developed an asymmetric outer membrane (OM) model and used molecular dynamics simulations to investigate the interaction dynamics of lipopolysaccharide (LPS)-containing OM with the polymyxins. It was discovered that polymyxin molecules penetrated the OM core sugar region but were trapped before entering the lipid A region due to a major free energy barrier at the LPS inner core and lipid A interface. Calcium ions were found to inhibit the penetration of polymyxins into the hydrophobic region of the OM by mediating cross-linking interactions between LPS molecules.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Sejeong Lee, Hagan Bayley
Summary: This study shows that the outer membrane lipid bilayer of Escherichia coli can be reconstructed as a droplet interface bilayer, which provides a physiologically realistic model for studying the properties of antibiotics. The findings demonstrate that the model outer membrane lipid bilayers differ from simple lipid bilayers in terms of translocation efficiency and stability.
Review
Immunology
Shuming Wang, Jiayi Guo, Yang Bai, Cai Sun, Yanhao Wu, Zhe Liu, Xiaofei Liu, Yanfeng Wang, Zhigang Wang, Yongmin Zhang, Huifang Hao
Summary: This paper reviews the applications of bacterial outer membrane vesicles in tumor vaccines and their interaction with host cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Zeinab Mohamed, Jung-Ho Shin, Surajit Ghosh, Abhishek K. Sharma, Ferra Pinnock, Samavi Bint E. Naser Farnush, Tobias Dorr, Susan Daniel
Summary: Research shows that a surface-supported membrane platform using outer membrane vesicles (OMVs) can mimic bacterial membrane features and study its interaction with antibacterial compounds. This platform maintains critical outer membrane features like fluidity, while also retaining complex membrane components like OMPs and LPS, crucial for studying membrane-mediated antibiotic resistance.
ACS INFECTIOUS DISEASES
(2021)
Review
Engineering, Biomedical
Wenlong Huang, Lingxi Meng, Yuan Chen, Zaiquan Dong, Qiang Peng
Summary: Antibiotic therapy is important, but its improper use and emergence of drug resistance have reduced its effectiveness. Therefore, developing more potent antimicrobial therapies is crucial. Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria have been used as active agents, drug carriers, and vaccine adjuvants, showing great potential.
ACTA BIOMATERIALIA
(2022)
Review
Immunology
Shannon M. Collins, Angela C. Brown
Summary: Bacterial outer membrane vesicles (OMVs) play key roles in pathogenesis by delivering biomolecules to host cells, while also facilitating intra-bacterial communication processes, making them promising antibiotic delivery vehicles.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Multidisciplinary Sciences
Craig R. R. MacNair, Man-Wah Tan
Summary: The survival of bacteria during antibiotic exposure is a complex phenomenon, and bacterial membrane vesicles (MVs) play a significant role as a protective layer. MVs not only reduce the efficacy of antibiotics, but also transmit resistance elements, making them a potential target for unconventional therapy.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Microbiology
Janine H. Peterson, Matthew Thomas Doyle, Harris D. Bernstein
Summary: Several antibacterial compounds have been found to inhibit BAM function through different mechanisms, affecting the biogenesis of outer membrane proteins in Gram-negative bacteria. These findings not only provide important insights for developing novel broad-spectrum antibiotics, but also valuable tools for future studies on BAM function.
Article
Chemistry, Multidisciplinary
Ilkoo Noh, Zhongyuan Guo, Jiarong Zhou, Weiwei Gao, Ronnie H. Fang, Liangfang Zhang
Summary: Vaccination with nanovaccines based on bacterial outer membrane has shown promising results in protecting against antibiotic-resistant infections. The cellular nanodiscs developed in this study effectively interact with antigen-presenting cells and can efficiently transport to lymph nodes. In a murine model, the nanovaccine elicits potent immune responses and confers protection against subsequent lung infection.
Review
Biochemistry & Molecular Biology
Federica Dell'Annunziata, Veronica Folliero, Rosa Giugliano, Anna De Filippis, Cristina Santarcangelo, Viviana Izzo, Maria Daglia, Massimiliano Galdiero, Carla Renata Arciola, Gianluigi Franci
Summary: Outer membrane vesicles (OMVs) released by Gram-negative bacteria are rounded nanostructures that can encapsulate biologically active toxins and virulence factors, and have been recently found to mediate horizontal gene transfer processes. Little is known about the packaging, release, and transfer mechanisms of OMVs, but they play an important role in HGT processes in bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Chengcheng Zhou, Zeyu Ding, Qiaoni Guo, Meijuan Jiang
Summary: This study employed an aggregation-induced emission luminogen (AIEgen) to indicate the interaction of antimicrobials with the outer membrane (OM) and cytoplasmic membrane (CM) of Gram-negative bacteria. The damaged extent of OM and CM caused by antimicrobials can be visually captured based on the variation of two emission colors of IQ-Cm. The probe may shed light on the understanding of the interaction between the membrane-active antimicrobials and cell envelope of Gram-negative bacteria and contribute to the future development of antimicrobials.
Article
Immunology
Daniel Hatlem, Mikkel Christensen, Nina K. Broeker, Per E. Kristiansen, Reidar Lund, Stefanie Barbirz, Dirk Linke
Summary: Alpha-helical coiled-coils are common protein structures found in all living organisms. Modified coiled-coil sequences have been widely used in biotechnology, vaccine development, and biochemical research to induce protein oligomerization and form self-assembled protein scaffolds. In this study, a trimeric variant of a peptide derived from the yeast transcription factor GCN4, called GCN4-pII, was shown to bind bacterial lipopolysaccharides (LPS) with high affinity. LPS molecules are toxic glycolipids found in the outer membrane of Gram-negative bacteria. The researchers also demonstrated the ability of GCN4-pII to break down LPS micelles in solution. These findings suggest that GCN4-pII and its derivatives could be used for novel LPS detection and removal solutions with important applications in the production and quality control of biopharmaceuticals and other biomedical products, where even trace amounts of residual LPS can be deadly.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Emma van Groesen, Cornelis J. Slingerland, Paolo Innocenti, Milos Mihajlovic, Rosalinde Masereeuw, Nathaniel Martin
Summary: Vancomycin binds to lipid II to exert its antimicrobial effect, but cannot pass through the outer membrane of Gram-negative bacteria. Conjugation of vancomycin with OM disrupting PMEN results in hybrid vancomyxins with improved activity against Gram-negative strains and maintained antimicrobial effect against Gram-positive strains. The hybrid antibiotics show reduced nephrotoxicity compared to clinically used polymyxin antibiotics.
ACS INFECTIOUS DISEASES
(2021)
Review
Microbiology
Min Xiao, Guiding Li, Hefeng Yang
Summary: Bacteria-host interaction is a common and intriguing biological phenomenon. Bacteria communicate with the host through membrane vesicles (MVs) and play important roles in bacterial pathogenesis. Research on the structure, function and therapeutic potential of MVs is significant for understanding and utilizing the interaction between bacteria and hosts.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Nicolo Paracini, Emanuel Schneck, Anne Imberty, Samantha Micciulla
Summary: Lipopolysaccharides (LPSs) are crucial components of Gram-negative bacterial outer membranes, and studying their structure and biophysical properties can provide insights into their interactions with antimicrobial compounds.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Md Shahadat Hossain, Jingjing Ji, Christopher J. Lynch, Miguel Guzman, Shikha Nangia, Davoud Mozhdehi
Summary: Recombinant nanoworms hold promise for a wide range of applications, but the molecular design principles for their assembly remain unclear. By incorporating posttranslational modifications to generate lipidated proteins with specific asymmetry, researchers demonstrated the ability to modulate material properties and program the assembly of proteins into adaptive nanoworms that transition morphologically in response to temperature stimuli.
Article
Oncology
Yongchang Zhang, Lianxi Song, Liang Zeng, Yi Xiong, Li Liu, Chunhua Zhou, Haiyan Yang, Zhan Wang, Qing Xia, Wenjuan Jiang, Qinqin Xu, Nong Yang
Summary: This study demonstrates the effectiveness and safety of the combination therapy of sintilimab plus docetaxel as a second-line treatment for NSCLC without targetable mutations after progression from first-line platinum-based chemotherapy. Patients with high PD-L1 expression in circulating tumor cells showed higher objective response rate (ORR) and longer progression-free survival (PFS) and overall survival (OS). Patients with PD-L1 < 1% and CD8 >= 1% expression from their baseline tissue samples also had higher ORR.
Article
Plant Sciences
Yaoyao Bian, Mei Xue, Xinlong Guo, Wenjuan Jiang, Ye Zhao, Zhaofeng Zhang, Xian Wang, Yongkang Hu, Qi Zhang, Wenliang Dun, Liang Zhang
Summary: CBG induces apoptosis and PML-RARA degradation in NB4 and NB4-R1 cells in a caspase-dependent manner by inhibiting the beta-catenin signaling pathway.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Hairui Fang, Sheng Li, Wenjuan Jiang, Huamin Zhao, Chenshuai Han, Jing Li, Gong Wang, Yuan Zhang, Shan Wang, Jin Deng, Bo Feng, Dong Wang
Summary: This paper presents a sensor for H2S gas detection in meat freshness evaluation, using a NiO-doped CuO composite derived from MOFs. The low temperature precursor hydrothermal method was found to have a guiding significance in improving the gas response performance. The sensor exhibited a high response rate and good selectivity to H2S gas, making it suitable for detecting meat spoilage under low temperature storage. This work provides a new idea for meat freshness detection in cold storage environments.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Computer Science, Artificial Intelligence
HaiRui Fang, Jin Deng, DongSheng Chen, WenJuan Jiang, SiYu Shao, MingCong Tang, JingJing Liu
Summary: This paper proposes a convolutional unit modified by transformer, which integrates transformer and CNN into a whole, and based on this unit, a series of novel lightweight diagnosis frameworks named SANet are proposed. It is demonstrated that SANet can complete the high-accuracy diagnosis task with less than three thousand parameters and has strong robustness to noise, as well as satisfactory results with few training samples.
ADVANCED ENGINEERING INFORMATICS
(2023)
Article
Chemistry, Multidisciplinary
Guomin Wang, Kaiwei Tang, Wenjuan Jiang, Qing Liao, Yong Li, Pei Liu, Yuzheng Wu, Mengting Liu, Huaiyu Wang, Bin Li, Jianzhong Du, Paul K. Chu
Summary: Adjustable electro-mechanical forces can effectively kill bacteria and adding electrical charges can enhance the antibacterial efficacy.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Jingjing Ji, Md Shahadat Hossain, Emily N. Krueger, Zhe Zhang, Shivangi Nangia, Britnie Carpentier, Mae Martel, Shikha Nangia, Davoud Mozhdehi
Summary: Lipidated proteins are hybrid biomaterials that combine the functional capabilities of proteins with precisely engineered nano-biomaterials. The thermoresponsive behavior of fatty-acid-modified elastin-like polypeptides (FAMEs) and the structural changes induced by temperature and molecular syntax were investigated using computational nanoscopy, spectroscopy, scattering, and microscopy. These findings provide insights into the biophysical consequences of lipidation in biology and facilitate the rational design of biomaterials and therapeutics.
Article
Chemistry, Physical
Jingjing Ji, Britnie Carpentier, Arindam Chakraborty, Shikha Nangia
Summary: The hydropathy of proteins has been a topic of interest for decades. Existing methods overlook the nanoscale topography of proteins in calculating their hydropathy. To overcome this limitation, we have developed a new scale called PARCH that evaluates the hydropathy of a residue based on the collective response of water molecules in the protein's first hydration shell. The PARCH scale calculations are computationally inexpensive and can aid in designing nanostructured surfaces, identifying hydrophilic and hydrophobic patches, and drug discovery.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Article
Biochemical Research Methods
Xiaochuan Li, Qixuan Qian, Wenjuan Jiang
Summary: A photochrmic triangle terthiophene dye with 2,4-dimethylthiazole attached showed regular photochromic properties under UV/Vis light irradiation alternately. The attachment of 2,4-dimethylthiazole significantly influenced both the photochromism and fluorescence of the dye. The dye could switch between ring-open and ring-closed forms, altering both color and fluorescence, during the photocyclization process in THF.
JOURNAL OF FLUORESCENCE
(2023)
Article
Multidisciplinary Sciences
Arturo Raya-Sandino, Kristen M. Lozada-Soto, Nandhini Rajagopal, Vicky Garcia-Hernandez, Anny-Claude Luissint, Jennifer C. Brazil, Guiying Cui, Michael Koval, Charles A. Parkos, Shikha Nangia, Asma Nusrat
Summary: Research has shown that Claudin-23 is enriched in luminal intestinal epithelial cells and regulates the paracellular permeability of ions and macromolecules by associating with other claudins. Computational modeling suggests that different combinations of claudins form complexes with unique pore architecture. These findings provide insights into the organization of tight junctions and their impact on epithelial barrier function.
NATURE COMMUNICATIONS
(2023)
Article
Computer Science, Artificial Intelligence
Jingna Si, Ziwei Tian, Dongmei Li, Lei Zhang, Lei Yao, Wenjuan Jiang, Jia Liu, Runshun Zhang, Xiaoping Zhang
Summary: Media convergence is a technology-driven media change. Applying media convergence to Chinese medicine clustering can exploit its advantages in combining media. This article proposes an approach called MCGEC for traditional Chinese medicine clinical data, which incorporates multiple modalities and uses media features for clustering.
CAAI TRANSACTIONS ON INTELLIGENCE TECHNOLOGY
(2023)
Article
Medicine, General & Internal
Xiao-Juan Yu, Tian Zhang, Zeng-Zhen Wei, Bin Gu, Ting Guo, Wen-Juan Jiang, Yue-Qin Shen, Dong Wang, Qian Wang, Jun Wang
Summary: The objective of this study was to analyze the value of serum miRNA-122 expression in the diagnosis, severity, and prognosis of Acute Cerebral Infarction (ACI), as well as the correlation mechanism of serum miRNA-122 on the proliferation and apoptosis of vascular endothelial cells in ACI. The results showed that serum miRNA-122 expression in patients with ACI was significantly higher than that in healthy controls, indicating its potential as a diagnostic marker for ACI. Furthermore, miRNA-122 may regulate ACI by inhibiting cell proliferation, increasing apoptosis, and inhibiting vascular endothelial cell regeneration through the CCNG1 channel.
Article
Nanoscience & Nanotechnology
Maryam Ramezani, Emily Elizabeth Labour, Jingjing Ji, Anand Utpal Vakil, Changling Du, Thalma Kabeyi Orado, Shikha Nangia, Mary Beth Browning Monroe
Summary: In this study, a library of antimicrobial polymers based on shape memory polyurethanes with honey-based phenolic acids was developed. The polymers demonstrated high transition temperatures and tunable mechanical and shape memory properties. They inhibited the growth of Staphylococcus aureus and Escherichia coli, as well as biofilm formation. Molecular dynamics simulations confirmed the interactions between the polymers and bacterial cell membranes. The antimicrobial properties of the polymers were retained even after long-term storage.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Geriatrics & Gerontology
Junxiu Liu, Linchi Jiao, Xin Zhong, Weifan Yao, Ke Du, Senxu Lu, Yuqiang Wu, Tianxin Ma, Junhui Tong, Mingyue Xu, Wenjuan Jiang, Yubao Wang, Miao He, Wei Xin, Mingyan Liu
Summary: Using bioinformatic analysis, we have identified PTAFR as a potential biomarker for early diagnosis and treatment of AD, which is associated with microglia-mediated microenvironment and can mediate inflammatory responses through the IL10-STAT3 pathway.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Meeting Abstract
Biophysics
Wenjuan Jiang, Yun Lyna Luo
BIOPHYSICAL JOURNAL
(2022)