期刊
DYES AND PIGMENTS
卷 80, 期 1, 页码 41-46出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2008.04.008
关键词
Enhanced fluorescence immunoassays; Silver island films; Seta dyes; Near-infrared fluorescent squaraine dyes; Total internal reflection; Sensors
资金
- Texas Emerging Technologies Fund
- NIH [HIS 004364]
- NSF [DBI-0649889]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG004364] Funding Source: NIH RePORTER
Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels. (C) 2008 Elsevier Ltd. All rights reserved.
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