Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Jean-Arthur Amalian, Tathagata Mondal, Evgeniia Konishcheva, Gianni Cavallo, Benoit Eric Petit, Jean-Francois Lutz, Laurence Charles
Summary: Sequence-defined synthetic polymers are being used as a medium to store information at the molecular level, with low storage capacity of readable chains being a current limitation. Ordering short oligomers on surfaces to compose long messages is an attractive alternative, and desorption electrospray ionization (DESI) is shown to be suitable for bidimensional data storage.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Article
Polymer Science
Ulric Conway, Alexander D. Warren, Christopher J. Arthur, Paul J. Gates
Summary: The main goals in the analysis of synthetic polymers include determining molecular weight, molecular weight distribution, and chemical structure. Matrix-assisted laser desorption/ionization mass spectrometry is a commonly used technique, and using carbon-based matrices with polyethylene glycol polymers doped with LiCl is an effective method for analyzing low molecular weight polymers.
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Yijia Wang, Amanda B. Hummon
Summary: MSI is a powerful imaging tool for drug discovery, capable of analyzing a wide range of molecules in diverse samples. Its potential has been demonstrated in 3D cell culture systems, providing insights into drug compound metabolism and distribution of metabolites. The application of MSI in patient-derived organoids for personalized medicine options is also discussed in this review.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Julian Schneemann, Karl-Christian Schaefer, Bernhard Spengler, Sven Heiles
Summary: This study presents a module that enhances the signal intensity of nonpolar compounds in MSI experiments. The module allows for the analysis of mouse tissue and caterpillar tissue, providing insight into the spatial distribution of nonpolar analytes.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Yi-Tzu Cho, Hung Su, Ching-Ying Wu, Jingyueh Jeng, Chi-Wei Lee, Deng-Chyang Wu, Tiao-Lai Huang, Jentaie Shiea
Summary: This study aimed to develop an efficient and non-invasive method for monitoring drugs and their metabolites through human skin. By combining probe sampling with thermal desorption-electrospray ionization tandem mass spectrometry, trace amounts of terbinafine secreted on patients' skin after ingestion could be characterized and monitored for 8 weeks. The detection and location of terbinafine through the skin suggest that this method is useful for rapidly and noninvasively collecting molecular information of ingested drugs for pharmacokinetic studies.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2022)
Article
Chemistry, Medicinal
Margaux Fresnais, Siwen Liang, Marius Breitkopf, Joshua Raoul Lindner, Emmanuelle Claude, Steven Pringle, Pavel A. Levkin, Konstantin Demir, Julia Benzel, Julia Sundheimer, Britta Statz, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: Desorption/ionization-mass spectrometric methods are advantageous for the analysis of solid biological matrices. The development of drug quantification assays using DESI has been challenging, but new prototype source setups show promise for targeted quantitative analyses.
Article
Medicine, Legal
K. Wiese Simonsen, P. Kriikku, G. Thelander, H. M. E. Edvardsen, S. Thordardottir, C. U. Andersen, A. K. Jonsson, J. Frost, D. J. Christoffersen, G. J. M. Delaveris, I. Ojanpera
FORENSIC SCIENCE INTERNATIONAL
(2020)
Article
Medicine, Legal
Philippe Lunetta, Pirkko Kriikku, Julius Tikka, Ilkka Ojanpera
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2020)
Article
Medicine, Legal
Pirkko Kriikku, Ilkka Ojanpera, Philippe Lunetta
AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY
(2020)
Article
Medicine, Legal
Samuel Mesihaa, Ilpo Rasanen, Ilkka Ojanpera
FORENSIC SCIENCE INTERNATIONAL
(2020)
Article
Substance Abuse
Claudia Mariottini, Pirkko Kriikku, Ilkka Ojanpera
Summary: This study investigated the role of emerging concomitant drugs in buprenorphine user deaths, finding a high prevalence of benzodiazepines, illicit drugs, and other substances in cases of buprenorphine poisoning. The study also highlighted the particularly unsafe drug use patterns in the younger age group, suggesting the need for targeted prevention strategies.
DRUG AND ALCOHOL DEPENDENCE
(2021)
Article
Biochemical Research Methods
Claudia Mariottini, Ilkka Ojanpera, Pirkko Kriikku
Summary: Evidence-based data shows that during the COVID-19 pandemic, the numbers of buprenorphine, amphetamine, and cannabis findings in post-mortem investigations have increased significantly, with the most noticeable rise seen in amphetamine across all age groups. These findings indicate the reality of increased risks of drug-related harm and the urgent need for significant actions to assist this vulnerable group of individuals.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Claudia Mariottini, Merja Gergov, Ilkka Ojanpera
Summary: A method using liquid chromatography-tandem mass spectrometry was developed for simultaneous quantification of buprenorphine and its metabolites in urine samples, without the need for a hydrolysis step. The method was able to cover the concentration ranges encountered in a clinical setting.
DRUG TESTING AND ANALYSIS
(2021)
Article
Medicine, Legal
Pirkko Kriikku, Ilkka Ojanpera
Summary: The study found that gabapentinoids are mainly implicated in fatal poisoning together with opioids, but in some cases, death may be caused by overuse of prescribed drugs or suicide. It is important to pay attention to different user groups who may be at risk of overdose by gabapentinoids.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Medicine, Legal
Pirkko Kriikku, Samu Pelkonen, Maija Kaukonen, Ilkka Ojanpera
Summary: Propranolol and metoprolol showed significant differences in post-mortem toxicology cases, with propranolol users more associated with psychiatric conditions and having higher risks of fatal poisonings, suicides, and drug abuse.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Claudia Mariottini, Pirkko Kriikku, Ilkka Ojanpera
Summary: Quantitative analysis of postmortem urine, rather than blood, may provide additional evidence for the diagnosis of fatal buprenorphine poisoning. This study found that urinary concentrations and metabolite ratios were significantly different between the buprenorphine poisoning and other causes of death groups, with higher concentrations and lower ratios observed in poisonings. Naloxone-related concentrations and ratios did not show significant differences between the groups.
DRUG TESTING AND ANALYSIS
(2022)
Article
Medicine, Legal
Pirkko Kriikku, Eija Kalso, Ilkka Ojanpera
Summary: This study investigated post-mortem oxycodone blood concentrations in oxycodone-related medico-legal cases in hospitals and nursing homes in Finland. It found that cancer patients had significantly higher oxycodone concentrations compared to patients with recent surgery or other diseases. Additionally, the poisoning groups had significantly higher oxycodone concentrations compared to the hospital patient groups.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Medicine, Legal
Petteri Oura, Antti Virtanen, Juho Nurkkala, Pirkko Kriikku, Ilkka Ojanperae
Summary: Local anesthetics injected into the femoral area can relieve pain in hip fracture patients. This study examined postmortem blood samples from ten medico-legal autopsy cases to determine the levels of local anesthetics in ipsilateral and contralateral femoral blood after hip fracture surgery. The results showed significantly higher levels of ropivacaine on the ipsilateral side compared to the contralateral side. These findings suggest caution in interpreting toxicology reports based on blood collected from the operated area.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Chemistry, Analytical
Pirkko Kriikku, Ilkka Ojanpera
Summary: Fatal reference concentrations of antidepressants and antipsychotics in poisonings were determined based on postmortem investigations in Finland. The study found consistent results for these drugs, providing useful reference values for interpretation in toxicology analysis.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)
Article
Medicine, Legal
Pirkko Kriikku, Ilkka Ojanpera, Philippe Lunetta
Summary: In Finland, we examined 17 fatal ethylene glycol poisonings and found that most of the deceased were male with a median age of 47 years. The vitreous humour glucose levels were above the limit of quantitation in all cases, suggesting the intake of ethylene glycol as an ethanol substitute.
Article
Biochemical Research Methods
Pirkko Kriikku, Aino Kankaanpaeae, Teemu Gunnar, Ilkka Ojanperae
Summary: This study compared and explored the trends of amphetamine, methamphetamine, and MDMA using drug-related deaths and wastewater-based epidemiology (WBE) indicators. It found a significant correlation between poisoning and drug consumption for all three stimulants and identified methamphetamine as the most fatal, followed by MDMA and amphetamine. Additionally, this study quantitatively investigated the fatal toxicity of amphetamine-type stimulants by comparing deaths with consumption estimates derived from WBE.
DRUG TESTING AND ANALYSIS
(2023)