Article
Biochemical Research Methods
Laurie De Wilde, Kris Roels, Koen Deventer, Peter Van Eenoo
Summary: The study developed and validated a method for detecting HIFs in urine samples using online solid-phase extraction liquid chromatography-tandem mass spectrometry, with features of high efficiency, automation, and high sample throughput.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Biochemical Research Methods
Alice Ameline, Laurie Gheddar, Jean-Sebastien Raul, Pascal Kintz
Summary: Letrozole, a reversible aromatase inhibitor used in cancer treatment, has been observed to be abused as a doping agent in high level athletes, even though it has no medical indications for men. A specific method has been developed to identify Letrozole in human hair, providing a more precise means for anti-doping agencies to detect its presence in athletes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Hideaki Ishii, Mariko Shibuya, Kanichi Kusano, Yu Sone, Takahiro Kamiya, Ai Wakuno, Hideki Ito, Kenji Miyata, Masayuki Yamada, Gary Ngai-Wa Leung
Summary: This study demonstrates the potential long-term detection of daprodustat and its metabolites in horse hair and provides insights into the mechanism of drug incorporation into hair. The major factors contributing to drug incorporation were found to be the maximum plasma concentrations and lipophilicities of the drugs. These findings are important for doping control and understanding drug incorporation into horse hair.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Bahare Makvandi, Anton Pohanka, Mats Bergstrom, Annica Borjesson, Mikael Lehtihet, Lena Ekstrom, Yufang Zheng
Summary: A LC-MS/MS method for measuring anabolic androgenic steroids in serum was developed, and it was found that serum could be a valid complementary matrix to urine samples for testing.
DRUG TESTING AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Yuling Yuan, Xuxiao Zhao, Huan Wei, Qiaoling Fei, Youxuan Xu, Jianghai Lu
Summary: Trimetazidine is an effective drug for treating chest pain, but it is banned by WADA due to its potential to enhance athletic performance. Through comprehensive studies, new metabolites and metabolic pathways of Trimetazidine have been discovered, providing potential biomarkers for detecting misuse.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Menghua Zhang, Xiaoxue Liu, Zhiyao Chen, Shenhao Jiang, Lin Wang, Min Tao, Liyan Miao
Summary: Therapeutic drug monitoring (TDM) of tyrosine kinase inhibitors (TKIs) and their active metabolites has proven to enhance treatment efficacy and safety. In this study, a sensitive and cost-effective ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination of six oral TKIs and two active metabolites in human plasma/serum. The method showed good linearity, precision, and accuracy, providing support for clinical practice.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Zhenhua Tian, Luping Sun, Bingqing Chi, Zhen Du, Xiumei Zhang, Yuecheng Liu, Honglei Zhou
Summary: This study investigated the chemical components of Angelica pubescens using ultra high performance liquid chromatography and high-resolution Orbitrap mass spectrometry. Neuraminidase inhibitors were screened by incubating neuraminidase with the extract of Angelica pubescens using affinity ultrafiltration methods. Thirteen small molecules were discovered to interact with neuraminidase for the first time. In vitro testing confirmed the neuraminidase inhibitory activity of certain compounds and the extract. Molecular docking analysis was conducted to predict potential docking positions. This study provides a reference for the medical substance basis in Angelica and the clinical usage of this drug.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Ryan Savill, Helge Baues, Emmely Voigt, Oliver Zierau, Detlef Thieme, Annekathrin Martina Keiler
Summary: The study investigates the biotransformation of synthetic testosterone derivatives in different cell culture models, showing variations in metabolic pathways among different cell lines. BG-1 and HepG2 cells exhibited the highest conversion rates in phase I metabolism, suggesting their potential as suitable models for the generation of metabolite reference collections.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Thomas Piper, Sonja Heimbach, Martin Adamczewski, Mario Thevis
Summary: The steroid profile is used in sports drug testing to detect misuse of endogenous steroids, with individual reference ranges compared to population-based thresholds via the Athlete Biological Passport. Different doping agents were investigated for their effects on the steroid profile, using various tests coupled with analytical approaches to understand compound impact, although predicting specific influences remains challenging.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Aoalheiour Dora Albertsdottir, Wim Van Gansbeke, Peter Van Eenoo, Michael Polet
Summary: The detection of anabolic androgenic steroids (AAS) is frequent among rule-breaking athletes. The long-term metabolites, particularly non-hydrolysed sulphated metabolites, have gained attention for their extended detection time. Liquid and gas chromatography-mass spectrometry (LC- and GC-MS) have been used to investigate their potential. However, the most promising metabolite on one technique may not exhibit the same behavior on the other. Consequently, a comparison was carried out to identify the most likely long-term sulphated metabolites of metenolone, mesterolone, and 17-alpha-methyltestosterone on four mass spectrometric instruments.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Koen Deventer, Michael Polet, Wim Van Gansbeke, Fiona Hooghe, Helen Van Hoecke, Peter Van Eenoo
Summary: The use of glucocorticosteroids in sports is restricted to certain non-systemic applications, with systemic use prohibited. Prednisolone is an effective treatment for nasal congestion and allergic rhinitis. However, the urinary concentration of nasally administered prednisolone may exceed current reporting limits, calling for a potential increase in these limits to avoid false-positive results.
DRUG TESTING AND ANALYSIS
(2021)
Article
Chemistry, Analytical
Andrzej Pokrywka, Daria Berezovska, Sylwia Lewandowska-Pachecka, Jaroslaw Krzywanski, Michal Rynkowski, Mariola Wicka, Dorota Kwiatkowska, Raphael Faiss
Summary: In its 2022 update, World Anti-Doping Agency (WADA) prohibited all injectable routes of administration of glucocorticoids (GCs) during in-competition periods. However, a study found that despite the new regulation, 8% of athletes declared the use of 30 different GC preparations during competition, with only 2 samples triggering adverse analytical findings.
Article
Biochemical Research Methods
Sergi Coll, Nuria Monfort, Elida Alechaga, Xavier Matabosch, Oscar J. Pozo, Clara Perez-Mana, Rosa Ventura
Summary: Prednisolone and prednisone are prohibited in sports competitions when administered by systemic routes, while they are allowed for therapeutic purposes by other routes. Concentrations of metabolites in urine were generally lower than 30 ng/ml after nonsystemic treatments, and high concentrations were observed within the first 24 hours after oral administration. New reporting levels and recommended washout periods were proposed based on the study data.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Xiaona Gan, Bo Peng, Liang Chen, Yanjun Jiang, Tingzhao Li, Bo Li, Xiaodong Liu
Summary: This study established a method using affinity ultrafiltration-liquid chromatography-mass spectrometry (UF-LC-MS) to identify the bioactive compounds in celery seed extracts that contribute to the inhibition of xanthine oxidase (XOD). Thirty-two compounds, including flavonoids and phenylpeptides, were identified. Enzyme activity assays demonstrated that celery seed extract and several compounds could dose-dependently inhibit XOD.
Article
Biochemical Research Methods
Camilla Regina de Souza Madeira, Mariana Millan Fachi, Victor Concentino, Alexandre de Fatima Cobre, Angela Cristina Leal Badaro Trindade, Alan Guilherme Goncalves, Roberto Pontarolo
Summary: Phosphodiesterase inhibitors (PDE5i) are widely used for the treatment of erectile dysfunction, requiring appropriate concentration levels. A new bioanalytical method was developed for simultaneous quantification of multiple PDE5i, offering important insights for future studies on pharmacokinetics and therapeutic monitoring.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
