Article
Biochemistry & Molecular Biology
Peter D. Giang, Luke R. Churchman, Jeanette E. Stok, Stephen G. Bell, James J. De Voss
Summary: Rhodococcus globerulus, a bacterium, can utilize eucalypt oil as its only source of carbon and energy. Two cytochromes P450 from this organism, CYP176A1 and CYP108N12, initiate the biodegradation of monoterpenes in the oil. A specific [2Fe-2S] ferredoxin redox partner called cymredoxin has been isolated and characterized for CYP108N12, and it has been found to improve the catalytic ability of CYP108N12 and support the activity of other P450s.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Medicinal
Yaoyao Cai, Qianmeng Lin, Zhousheng Jin, Fangfang Xia, Yingchao Ye, Yun Xia, Thomas J. Papadimos, Quanguang Wang, Guoxin Hu, Jianping Cai, Limei Chen
Summary: Cytochrome P450 3A4, a highly polymorphic enzyme, metabolizes a significant portion of therapeutic drugs. This study evaluated the catalytic activities of different CYP3A4 variants towards oxycodone and identified specific variants that affect drug clearance rates. Understanding the genotype-phenotype relationship for oxycodone metabolism may provide valuable insights for personalized precision medicine.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Review
Pharmacology & Pharmacy
An Le, Kai J. Huang, Lauren R. Cirrincione
Summary: This article synthesizes available in vitro and animal model data with pharmacological concepts in transgender medicine to predict potential effects of sex steroids on drug-metabolizing enzymes and their relationship with potential hormone-drug interactions.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Thomas Lautier, Derek J. Smith, Lay Kien Yang, Xixian Chen, Congqiang Zhang, Gilles Truan, Nic D. Lindley
Summary: Cytochromes P450, a superfamily of monooxygenases, have versatile substrate specificity. Metabolic engineering can exploit this feature to unlock new metabolic pathways. However, expressing cytochromes P450 in a different host can be challenging. This study focused on optimizing the in vivo activity of CYP97H1 in Escherichia coli to produce fi-cryptoxanthin, a difficult carotenoid intermediate. Through engineering the N-terminal region of CYP97H1, identifying compatible redox partners, defining the optimal cellular background, and adjusting culture and induction conditions, the production of fi-cryptoxanthin increased by 400 times compared to the initial strain, reaching 2.7 mg/L and accounting for 20% of total carotenoid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tamara Heintze, Kathrin Klein, Ute Hofmann, Ulrich M. Zanger
Summary: The use of lentiviral transduction to deliver Cas9 and sgRNAs in HepaRG cells resulted in efficient knockdown of POR and revealed differential effects on drug-metabolizing CYP enzymes. Knockdown of POR also affected the expression of CYPs at mRNA and protein levels. The study highlights the importance of POR/NADPH- and CYB5/NADH-electron transport systems in influencing human drug metabolism, with implications for future research on hepatic functions.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Libo Zhang, Dumei Ma, Yingwu Yin, Qian Wang
Summary: The study demonstrates the use of a novel class of benzene containing small molecules to improve the activity of OleT enzyme, leading to a significant increase in product yield in enzymatic reactions. These findings shed light on the potential application of small molecules to enhance the catalytic efficiency of OleT for future olefin production.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemical Research Methods
Danilo Correddu, Gianluca Catucci, Daniele Giuriato, Giovanna Di Nardo, Alberto Ciaramella, Gianfranco Gilardi
Summary: Self-sufficient cytochromes P450 of the sub-family CYP116B, particularly CYP116B5, have attracted attention for their ability to catalyze challenging reactions. However, they are often unstable and have limited activity. In this study, the native reductase domain of CYP116B5 was replaced with a monomeric sarcosine oxidase (MSOX) to generate a chimeric enzyme (CYP116B5-SOX) capable of producing H2O2. CYP116B5-SOX exhibited significantly higher catalytic activity compared to CYP116B5-fl and CYP116B5-hd, indicating its potential for future biotechnological applications.
BIOTECHNOLOGY JOURNAL
(2023)
Review
Chemistry, Analytical
Li Mi, Ziteng Wang, Wenge Yang, Chaofeng Huang, Bin Zhou, Yonghong Hu, Songqin Liu
Summary: Cytochrome P450 (CYP450s) are a large superfamily of enzymes that catalyze versatile reactions introducing oxygen into molecules. However, the current biotechnologies are hindered by limited oxygen availability. This review summarizes methods for CYP450s immobilization, activity detection, and synthesis of complex products, and introduces a novel approach using a gas-solid-liquid interface to overcome oxygen limitation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Diana Dimunova, Petra Matouskova, Martina Navratilova, Linh Thuy Nguyen, Martin Ambroz, Ivan Vokral, Barbora Szotakova, Lenka Skalova
Summary: This study found that the environmental circulation of veterinary anthelmintics may contribute to drug resistance in parasitic nematodes, as it improves their ability to deactivate the drugs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Liping Chen, Ping Guo, Wenxue Li, Xinhang Jiang, Qun Zhao, Daochuan Li, Qing Wang, Yongmei Xiao, Xiumei Xing, Yaqin Pang, Michael Aschner, Lihua Zhang, Wen Chen
Summary: Protein phosphatase 2A (PP2A) is an enzyme that plays various roles in biological processes, but its specific actions in many critical pathways are unclear. Through a study on a mouse model, it was found that PP2A is involved in the regulation of liver metabolism, and specific PP2A enzymes contribute to the induction of metabolic enzymes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Menna Al-Adl, Afaf El-Said, Ahmed EL-Sebaie, Sherif Refaat, Magdy M. Youssef
Summary: This study found that the CYP1A1*2A (T3801C, rs4646903) single nucleotide polymorphism (SNP) may be a risk factor for developing B-CLL, and the impact of this SNP on the disease progression and the clinical outcome.
Article
Biotechnology & Applied Microbiology
Hongyan Pan, Shangfeng Chang, Ying Qu, Mingxin Liu, Wei Tian, Zunxue Chang
Summary: In this study, hydrocortisone was successfully synthesized from progesterone for the first time using engineered Escherichia coli strains with assembled P450s in an artificial cascade reaction. The efficient biosynthetic pathway of deoxycortisone from progesterone was constructed, and a three-P450-modules co-expression system of E. coli C43(DE3) named hyg4 was developed. The total production of hydrocortisone reached 16.82 μmol/L within 24 hours.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Review
Medicine, Research & Experimental
Mohammed G. Maslub, Mahasen A. Radwan, Nur Aizati Athirah Daud, Abubakar Sha'aban
Summary: This article reviews the association between genetic variations in CYP3A4/5 and the response to atorvastatin therapy globally, including its pharmacokinetics and the risk of adverse reactions. It also highlights the need for further studies in the Egyptian population.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Loes P. M. Duivenvoorde, Jochem Louisse, Nicole E. T. Pinckaers, Tien Nguyen, Meike van der Zande
Summary: Flow conditions have been shown to improve the longevity and functionality of primary hepatocytes, but their impact on HepaRG cells remains largely unknown. This study found that HepaRG cells cultured under flow conditions in an organ-on-chip device, as well as cells cultured under static conditions with HEPES-supplemented medium, exhibited more stable gene expression levels. The cells cultured in the OOC-device showed relatively high baseline CYP1 activity and gene expression levels similar to human primary hepatocytes, making them a promising in vitro model for studying hepatoxicity.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Xue-Yan Dai, Jia Lin, Shi-Yong Zhu, Jian-Ying Guo, Jia-Gen Cui, Jin-Long Li
Summary: The study revealed that lycopene (LYC) can alleviate the neurotoxicity caused by atrazine (ATR) by modulating nuclear receptors and CYP450. ATR induced oxidative stress and inflammatory damage, disrupted the balance of CYP450, but these effects were reversed by LYC pretreatment.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
T. Aomori, Y. Fujita, K. Obayashi, H. Sato, K. Kiyotani, K. Nakamura, T. Nakamura, K. Yamamoto
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS
(2014)
Article
Medicine, General & Internal
Hirotoshi Iihara, Mototsugu Shimokawa, Masakazu Abe, Yoh Hayasaki, Yukiyoshi Fujita, Yuki Nagasawa, Michiru Sakurai, Rie Matsuoka, Akio Suzuki, Kenichiro Morishige
Article
Medicine, General & Internal
Hirotoshi Iihara, Mototsugu Shimokawa, Takenobu Gomyo, Yukiyoshi Fujita, Tsutomu Yoshida, Norihiko Funaguchi, Koichi Minato, Daizo Kaito, Tomohiro Osawa, Momoko Yamada, Chiemi Hirose, Akio Suzuki, Yasushi Ohno
Article
Oncology
Hirotoshi Iihara, Mototsugu Shimokawa, Yoh Hayasaki, Yukiyoshi Fujita, Masakazu Abe, Motoki Takenaka, Senri Yamamoto, Takahiro Arai, Michiru Sakurai, Minako Mod, Kazuto Nakamura, Nobuhiro Kado, Saki Murase, Ryuichi Shimaoka, Akio Suzuki, Ken-ichirou Morishige
GYNECOLOGIC ONCOLOGY
(2020)
Article
Chemistry, Medicinal
Hiroki Kamioka, Takumi Tomono, Atsushi Fujita, Ryoichi Onozato, Misa Iijima, Shigeru Tsuchida, Takahiro Arai, Yukiyoshi Fujita, Xieyi Zhang, Kentaro Yano, Takuo Ogihara
JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Article
Oncology
Chizuru Sakai, Mototsugu Shimokawa, Hirotoshi Iihara, Yukiyoshi Fujita, Shinnosuke Ikemura, Chiemi Hirose, Mie Kotake, Norihiko Funaguchi, Takenobu Gomyo, Hisao Imai, Jun Hakamata, Daizo Kaito, Koichi Minato, Takahiro Arai, Hitoshi Kawazoe, Akio Suzuki, Yasushi Ohno, Hiroyuki Okura
Summary: The study demonstrated that prophylactic antiemetic therapy with a low dose of 5 mg olanzapine in combination with granisetron and dexamethasone showed durable efficacy and acceptable safety profile in patients with thoracic malignancies.
Article
Oncology
Senri Yamamoto, Hirotoshi Iihara, Ryuji Uozumi, Hitoshi Kawazoe, Kazuki Tanaka, Yukiyoshi Fujita, Masakazu Abe, Hisao Imai, Masato Karayama, Yoh Hayasaki, Chiemi Hirose, Takafumi Suda, Kazuto Nakamura, Akio Suzuki, Yasushi Ohno, Ken-ichirou Morishige, Naoki Inui
Summary: The study found that antiemetic regimens containing low-dose (5 mg) olanzapine could be effective and safe for patients receiving carboplatin-based chemotherapy.
Article
Medicine, General & Internal
Hiromi Nishiba, Hisao Imai, Yukiyoshi Fujita, Eriko Hiruta, Takashi Masuno, Shigeki Yamazaki, Hajime Tanaka, Teruhiko Kamiya, Masako Ito, Satoshi Takei, Masato Matsuura, Junnosuke Mogi, Koichi Minato, Kyoko Obayashi
Summary: A multicenter, retrospective study was conducted to assess the effects of naldemedine on opioid-induced constipation in cancer patients. The results showed that naldemedine significantly increased the number of defecations and its efficacy and safety in clinical practice were comparable to prospective studies.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Teruhiko Kamiya, Hisao Imai, Yukiyoshi Fujita, Eriko Hiruta, Takashi Masuno, Shigeki Yamazaki, Hajime Tanaka, Mitsuru Sandoh, Satoshi Takei, Kazuya Arai, Hiromi Nishiba, Junnosuke Mogi, Shiro Koizuka, Taeko Saito, Kyoko Obayashi, Kyoichi Kaira, Koichi Minato
Summary: This study aimed to evaluate the efficacy and safety of naldemedine in patients with hepatobiliary pancreatic cancer receiving opioid analgesics. The results showed that naldemedine effectively increased the frequency of bowel movements in patients with minimal side effects, making it a viable treatment option for these patients.
MEDICINA-LITHUANIA
(2023)
Article
Infectious Diseases
Takashi Ohashi, Yukiyoshi Fujita, Hiroyuki Irisawa, Hidemasa Nakaminami, Takahiro Arai, Masumi Takahashi, Emi Momiyama, Naoya Murata, Kayoko Murayama, Taeko Saito
Summary: The study demonstrates that ABK is effective and safe in treating patients with FN and concurrent pneumonia caused by antimicrobial-resistant Gram-positive cocci. It may also be effective in patients who do not respond to other anti-MRSA drugs. Therefore, ABK could be beneficial in the treatment of pneumonia caused by antimicrobial-resistant Gram-positive cocci in patients with FN.
INFECTION AND CHEMOTHERAPY
(2022)
Review
Medicine, General & Internal
Eriko Hiruta, Yukiyoshi Fujita, Hisao Imai, Takashi Masuno, Shigeki Yamazaki, Hajime Tanaka, Teruhiko Kamiya, Masako Ito, Satoshi Takei, Masato Matsuura, Hiromi Nishiba, Junnosuke Mogi, Mie Kotake, Shiro Koizuka, Koichi Minato
Summary: This study investigated the treatment patterns of naldemedine in real-world clinical practice and found that, despite including patients not typically included in clinical trials, naldemedine showed similar efficacy and safety profiles in clinical practice as in clinical trials.
MEDICINA-LITHUANIA
(2021)
Article
Medicine, General & Internal
Takahiro Arai, Yukiyoshi Fujita, Hisao Imai, Hiroe Matsumoto, Miho Yamazaki, Eriko Hiruta, Yuka Suzuki, Hitoshi Ojima, Hisashi Hosaka, Koichi Minato, Taeko Saito
Summary: The association between seasons and rash incidence among patients with cancer treated with anti-EGFR antibodies was evaluated in this retrospective study. The findings suggest a higher incidence of grade >=2 rashes in male patients during summer compared to other patient groups.
MEDICINA-LITHUANIA
(2021)
Meeting Abstract
Pharmacology & Pharmacy
Hiroki Kamioka, Takumi Tomono, Kentaro Yano, Yukiyoshi Fujita, Atsushi Fujita, Ryoichi Onozato, Misa Iijima, Shigeru Tsuchida, Takahiro Arai, Takuo Ogihara
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Meeting Abstract
Oncology
Hironobu Hashimoto, Masakazu Abe, Masahiko Nakao, Hideaki Mizutani, Yasuhiko Sakata, Yukiyoshi Fujita, Tomoyasu Nishimura, Katsuya Hirano, Hideaki Okada, Naoki Inui, Yukio Sakata, Hirotoshi Iihara, Sadamoto Zenda, Yosuke Uchitomi, Takuhiro Yamaguchi, Yukari Hoshina, Takako Yanai, Satoru Iwasa, Noboru Yamamoto, Yuichiro Ohe
JOURNAL OF CLINICAL ONCOLOGY
(2019)
Meeting Abstract
Oncology
Yukiyoshi Fujita, Eiji Hoshino, Naoko Kawashima, Taeko Saito, Kouichi Minato, Yaeko Mishima
ANNALS OF ONCOLOGY
(2014)