Evaluation of Hepatoprotective Efficacy of Majoon-e-Dabeed-ul-ward Against Acetaminophen-Induced Liver Damage: A Unani Herbal Formulation

A Unani herbal formulation known as Majoon-e-Dabeed-ul-ward (MD) was evaluated for hepatoprotective effect against acetaminophen (APAP; 2 g/kg p.o.)-induced liver damage. The latter was evidenced by elevated levels of aspartate transaminase (AST), alanine transaminase (ALT), serum alkaline transaminase (SALP), lactate dehydrogenase (LDH), bilirubin, albumin, urea, and creatinine in experimental animals. Increased levels of lipid peroxidation were associated with a concomitant decline in reduced glutathione levels, adenosine triphosphatase (ATPase), and glucose-6-phosphatase (G-6-Pase) caused by APAP treatment. Treatment with MD (250,500, and 1,000 mg/kg p.o.) reverse the altered levels of AST, ALT, SALP, LDH, bilirubin, albumin, urea, and creatinine in a dose-dependent manner. Significant restoration was found in LPO, GSH content and metabolic enzymes (ATPase and G-6-pase) were seen after therapy. The herbal formulation, MD showed hepatoprotective efficacy against APAP-induced liver damage. Drug Dev Res 72: 346-352, 2011. (C) 2010 Wiley-Liss, Inc.