Article
Biochemistry & Molecular Biology
Zhaoyuan Zhu, Li Zhang, Ruilong Sheng, Jian Chen
Summary: A novel cationic cholesterol lipid derivative (CEL) was developed as a non-viral siRNA delivery carrier. Well-organized CEL/siRNA nanocomplexes were prepared using microfluidic-assisted assembly and exhibited good stability, low cytotoxicity, high siRNA delivery efficiency, and rapid cellular uptake through caveolae/lipid raft-mediated endocytosis pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mayuko Suzuki, Kouta Iwaki, Moeki Kikuchi, Kei Fujiwara, Nobuhide Doi
Summary: The study found that the fusogenic peptide S19 enhances the endosomal escape efficiency of TAT-fused proteins. Mutagenesis analysis showed that the amino acids with high beta-sheet forming propensities in S19 are important for the intracellular uptake of S19-TAT fusion proteins. The appropriate linking position and order of S19 and TAT peptides were also investigated, and both the previous C-terminal S19-TAT tag and the N-terminal TAT-S19 tag promote the cytoplasmic delivery of the fusion protein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Zhili Yu, Xiaojuan Zhang, Xing Pei, Weiran Cao, Junxiao Ye, Jianxin Wang, Lu Sun, Fei Yu, Jiancheng Wang, Nan Li, Kyuri Lee, Stefan Barth, Victor C. Yang, Huining He
Summary: In this study, antibodies and siRNAs were successfully conjugated using a multifunctional peptide as a linker, allowing for the specific delivery of siRNAs to target tumor cells. The released siRNA entered targeted cells via cellular penetrating peptide, resulting in improved gene silencing efficiency. The designed ARCs achieved approximately 66.7% EGFP downregulation efficiency in nude mice xenografted with the HCT116-EGFP tumor model, demonstrating a prospective choice for safe and efficient siRNA delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Kenta Shinga, Takahiro Iwata, Kazuya Murata, Yoko Daitoku, Junya Michibata, Jan Vincent V. Arafiles, Kentarou Sakamoto, Misao Akishiba, Tomoka Takatani-Nakase, Seiya Mizuno, Fumihiro Sugiyama, Miki Imanishi, Shiroh Futaki
Summary: This study presents a new cell-permeable ACAL peptide, L17ER4, that efficiently delivers proteins into cells. Compared to other peptides, L17ER4 exhibits higher delivery efficacy and can induce gene recombination in vivo.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
David Salehi, Saghar Mozaffari, Khalid Zoghebi, Sandeep Lohan, Dindyal Mandal, Rakesh K. Tiwari, Keykavous Parang
Summary: A series of cyclic and linear peptides containing arginine and either tryptophan or diphenylalanine residues were synthesized and evaluated for their molecular transporter efficiency. Among them, the [DipR](5) peptide showed significantly improved uptake of small molecules and negatively charged molecules, such as siRNA and phosphopeptide, suggesting its potential as an efficient membrane transporter.
Article
Chemistry, Multidisciplinary
Seung-Eun Chong, Jae Hoon Oh, Kyungjin Min, Sohyun Park, Sejong Choi, Joon Hyung Ahn, Dahyun Chun, Hyung Ho Lee, Jaehoon Yu, Yan Lee
Summary: A new vehicle is designed for intracellular delivery of antibodies at nanomolar concentrations by combining Z domain with LK sequences. The delivered IgGs can control targeted intracellular signaling pathway, showing potential for future therapeutic development.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Pharmacology & Pharmacy
Tejinder Singh, Dong Hyun Kang, Tae Wan Kim, Hye Jeong Kong, Jae Sung Ryu, Seob Jeon, Tae Sung Ahn, Dongjun Jeong, Moo Jun Baek, Jungkyun Im
Summary: The octaarginine-oxaliplatin conjugate successfully delivered oxaliplatin into colon cancer cells at low concentrations, showing high antitumor activity in both in vitro and in vivo studies. This strategy may provide a new approach for designing oxaliplatin derivatives with improved therapeutic capabilities.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Megane Bornerie, Anais Brion, Gilles Guichard, Antoine Kichler, Celine Douat
Summary: Tailored helical CPFs with specific end-groups (pyridylthio- or n-octyl-ureas) have been reported as effective molecular systems in combination with helper lipids to intracellularly deliver biologically-relevant siRNA.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biophysics
Diego Salas Sanzana, Emilia Flores Faundez, Jaime Melendez, Marco Soto-Arriaza
Summary: The functionalization of liposomes with snake venom phospholipase A2 peptides has been found to enhance the encapsulation and cytotoxicity of doxorubicin, leading to improved antitumor efficacy in HeLa cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yifan Yang, Haichao Zhu, Dingkang Liu, Hong Luo, Ruilong Chang, Yue Ji, Wenbing Yao, Jun Yin, Xiangdong Gao
Summary: SCP has emerged as a novel intracellular delivery system with enhanced efficiency and serum tolerance. However, its application is limited due to cationic charge and non-specificity. Researchers have engineered an activatable SCP (ASCP) with pH-sensitive charge shielding sequence, protease cleavage site, and SCP. This system reduces non-specific binding and delivers various cargo into the cytosol both in vitro and in vivo. The ASCP fusion protein enables co-delivery of therapeutic cargos for optimal outcomes and paves the way for combination cancer therapies.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Medicine, Research & Experimental
Pranjita Zantye, Soniya Shende, Sutapa Roy Ramanan, Indrani Talukdar, Meenal Kowshik
Summary: The study demonstrated the synthesis of self-fluorescent, cell-penetrating peptide-functionalized hydroxyapatite nanoparticles (R8HNPs) for RNA interference delivery in stem cells, showing good biocompatibility and delivery efficiency, achieving 80% silencing of the pluripotency marker gene Oct4 in R1 ESCs.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Wenjie Lang, Si Si Liew, Shuyi Wang, Dawei Hong, Liquan Zhu, Shubo Du, Linye Jiang, Shao Q. Yao, Jingyan Ge
Summary: Monitoring gene delivery has significant benefits in gene therapy. A nanoquencher system was developed in this study, which not only improves the efficiency of gene delivery polymers with minimal endolysosomal trapping, but also enables monitoring the release of the gene from the vehicle in live cells. This research expands the application of CPDs as promising tools in gene delivery.
CHEMICAL COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Shixian Lv, Meilyn Sylvestre, Alexander N. Prossnitz, Lucy F. Yang, Suzie H. Pun
Summary: Peptides as promising cancer therapeutics have shown robust antitumor efficacy, but the translation into clinical therapies is hindered by inherent limitations in their structure, hindering intracellular delivery. Strategies to engineer polymeric materials for increased peptide delivery efficiency, especially cytosolic delivery, play a crucial role in potentiating peptide-based cancer therapies. Future opportunities for peptides in cancer treatment lie in the design of polymer nanocarriers for optimized peptide delivery.
Article
Pharmacology & Pharmacy
Muhammad Imran Sajid, Dindyal Mandal, Naglaa Salem El-Sayed, Sandeep Lohan, Jonathan Moreno, Rakesh Kumar Tiwari
Summary: Recent approvals of siRNA-based products have led to exploration of siRNA as a potential treatment option for cancer and other diseases. This study introduces a series of oleyl-conjugated histidine-arginine peptides as a promising nonviral siRNA delivery tool.
Article
Biochemistry & Molecular Biology
Minami Matsuura, Mariko Ohshima, Yuki Hiruta, Tomohiro Nishimura, Kenichi Nagase, Hideko Kanazawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemical Research Methods
Kohei Okubo, Koji Ikeda, Ayaka Oaku, Yuki Hiruta, Kenichi Nagase, Hideko Kanazawa
JOURNAL OF CHROMATOGRAPHY A
(2018)
Article
Biochemistry & Molecular Biology
Kenichi Nagase, Momoko Hasegawa, Eri Ayano, Yoshie Maitani, Hideko Kanazawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Jiatu Li, Taisei Kaku, Yuki Tokura, Ko Matsukawa, Kenta Homma, Taihei Nishimoto, Yuki Hiruta, Aya Mizutani Akimoto, Kenichi Nagase, Hideko Kanazawa, Seimei Shiratori
Article
Chemistry, Multidisciplinary
Minami Maekawa-Matsuura, Kei Fujieda, Yutaro Maekawa, Tomohiro Nishimura, Kenichi Nagase, Hideko Kanazawa
Article
Chemistry, Analytical
Kenichi Nagase, Maria Watanabe, Fumihiko Zen, Hideko Kanazawa
ANALYTICA CHIMICA ACTA
(2019)
Article
Chemistry, Analytical
Kenichi Nagase, Sakiko Kitazawa, Sota Yamada, Aya Mizutani Akimoto, Hideko Kanazawa
ANALYTICA CHIMICA ACTA
(2020)
Article
Biophysics
Kenichi Nagase, Naho Uchikawa, Tadashi Hirotani, Aya Mizutani Akimoto, Hideko Kanazawa
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Article
Biochemistry & Molecular Biology
Kento Takaya, Ayano Sunohara, Noriko Aramaki-Hattori, Shigeki Sakai, Keisuke Okabe, Hideko Kanazawa, Toru Asou, Kazuo Kishi
Summary: This study analyzed the expression and localization of Wnt proteins in a mouse fetal-wound-healing model and found their association with scar formation. The results indicated that Wnt signaling may be involved in wound healing and scar formation. Regulation of Wnt signaling could be a possible mechanism to control scar formation.
Article
Biophysics
Kenichi Nagase, Akari Okada, Junnosuke Matsuda, Daiju Ichikawa, Yutaka Hattori, Hideko Kanazawa
Summary: In this study, a thermoresponsive cationic block copolymer brush-grafted beads were developed as packing materials for ADSC separation columns. These columns can adsorb ADSCs at 37 degrees C and elute them at 4 degrees C, while other cells are not adsorbed. This separation column can be used to isolate ADSCs without cell surface modification, while maintaining cell activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Engineering, Biomedical
Kenichi Nagase, Kaichi Yamazaki, Yutaro Maekawa, Hideko Kanazawa
Summary: Temperature-modulated exosome-capturing materials were developed using thermoresponsive polymers and peptides with affinity for exosomes. The copolymer-grafted silica beads successfully captured and released targeted exosomes via temperature modulation.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Kenichi Nagase, Naoto Kojima, Mitsuaki Goto, Toshihiro Akaike, Hideko Kanazawa
Summary: In this study, a hepatocyte cell separation material was developed using a thermoresponsive polymer and a polymer with high affinity to hepatocytes. The temperature modulation allowed for the effective separation of hepatocytes from other cells. This material holds significance for cell therapy and liver tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Materials Science, Biomaterials
Kenichi Nagase, Goro Edatsune, Yuki Nagata, Junnosuke Matsuda, Daiju Ichikawa, Sota Yamada, Yutaka Hattori, Hideko Kanazawa
Summary: A temperature-modulated mesenchymal stem cell separation column was developed in this study, allowing for the adsorption and elution of MSCs through temperature changes. The column successfully purified MSCs while retaining cellular activity, demonstrating its usefulness for MSC separation without cell modification.
BIOMATERIALS SCIENCE
(2021)
Article
Materials Science, Biomaterials
Kenichi Nagase, Masaki Shimura, Ruka Shimane, Kengo Hanaya, Sota Yamada, Aya Mizutani Akimoto, Takeshi Sugai, Hideko Kanazawa
Summary: A novel cell separation method using a thermoresponsive block copolymer brush with an affinity peptide was developed in this study, allowing for selective endothelial cell adhesion and detachment without cell modification. The method showed specificity in cell separation and could be conducted using a simple procedure, making it a promising tool for cell purification in tissue engineering and cell transplantation therapy.
BIOMATERIALS SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Taihei Nishimoto, Ko Matsukawa, Kenichi Nagase, Hideko Kanazawa, Aya Mizutani Akimoto, Ryo Yoshida
CHEMICAL COMMUNICATIONS
(2020)