期刊
DOMESTIC ANIMAL ENDOCRINOLOGY
卷 45, 期 3, 页码 122-125出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.domaniend.2013.06.006
关键词
miRNAs; Follicles; Anovulatory; Follicular fluid; Mares
资金
- Institute Strategic Grant Funding from the Biotechnology and Biological Sciences Research Council, UK
- BBSRC [BBS/E/D/20221656] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/D/20221656] Funding Source: researchfish
Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian follicle development The objective of this study was to gain insight on the involvement of these miRNAs during follicle maturation. Follicular fluid was aspirated from dominant follicles (>32 mm) during the ovulatory season (July to October) and the anovulatory season (January to March) in each of 5 mares, and the levels of steroids, IGF1, and miRNAs were analyzed by immunoassays and quantitative PCR. Levels of progesterone, testosterone, and IGF1 were lower (P <= 0.05) in anovulatory than in ovulatory follicles. Relative to ovulatory follicles, anovulatory follicles had higher (P < 0.05) mean levels of miR-21, miR-23b, miR-378, and miR-202 and tended to have higher (P = 0.06) levels of miR-145. Levels of miR-224 and miR-383 could not be detected in follicular fluid. These novel results indicate a physiological association between increases in follicular miRNA levels and seasonal anovulation in mares; further studies should elucidate the precise involvement of miR-21, miR-23b, miR-145, miR-378, and miR-202 in follicle maturation in the mare. (c) 2013 Elsevier Inc. All rights reserved.
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