期刊
JOURNAL OF PHARMACOLOGICAL SCIENCES
卷 128, 期 3, 页码 125-130出版社
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1016/j.jphs.2015.06.002
关键词
Adenosine receptor; Metabotropic glutamate receptor; FRET; GPCR; cAMP
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- MEXT
- Foundation of Strategic Research Projects in Private Universities from MEXT [S1311011]
- [KAKENHI 23700390]
- [26460707]
- [26430012]
- [23300128]
- [26670127]
- Grants-in-Aid for Scientific Research [26460707, 26430012] Funding Source: KAKEN
The adenosine A1 receptor (A1R) is a G protein-coupled receptor (GPCR) for adenosine, a ubiquitous neuromodulator, and thus regulates neuronal excitability, as well as arousal and sensitivity to pain. In addition, we have previously described a new mode of action for A1R: in cerebellar Purkinje cells, its activation attenuates neuronal responses to glutamate, as mediated by the type-1 metabotropic glutamate receptor (mGluR1). mGluR1 is also a GPCR, and elicits such responses as long-term depression of the postsynaptic response to glutamate, a cellular basis for cerebellar motor learning. Here, we explore in greater detail the interaction between A1R and mGluR1 using non-neuronal cells. Co-immunoprecipitation and Forster resonance energy transfer (FRET) analysis reveal that A1R and mGluR1 form a complex. Furthermore, we found that mGluR1 activation inhibits A1R signaling, as measured by changes in intracellular cAMP. These findings demonstrate that A1R and mGluR1 have the intrinsic ability to form a heteromeric complex and mutually modulate signaling. This interaction may represent a new form of intriguing GPCR-mediated cellular responses. (C) 2015 The Authors. Production and hosting by Elsevier B.V.
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