期刊
DNA REPAIR
卷 21, 期 -, 页码 140-147出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2014.04.006
关键词
Arabidopsis thaliana; AtMMS21; DNA damage response; Homologous recombination
资金
- National Natural Science Foundation of China [31170269, U1201212]
- Education Department of Guangdong Province [2012CXZD0019]
- Natural Science Foundation of Guangdong [S2012020011032]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
DNA damage is a significant problem in living organisms and DNA repair pathways have been evolved in different species to maintain genomic stability. Here we demonstrated the molecular function of AtMMS21, a component of SMC5/6 complex, in plant DNA damage response. Compared with wild type, the AtMMS21 mutant plants show hypersensitivity in the DNA damaging treatments by MMS, cisplatin and gamma radiation. However, mms21-1 is not sensitive to replication blocking agents hydroxyurea and aphidicolin. The expression of a DNA damage response gene PARP2 is upregulated in mms21-1 under normal condition, suggesting that this signaling pathway is constitutively activated in the mutant. Depletion of ATAXIA-TELANGIECTASIA MUTATED (ATM) in mms21-1 enhances its root growth defect phenotype, indicating thatATM and AtMMS21 may play additive roles in DNA damage pathway. The analysis of homologous recombination frequency showed that the number of recombination events is reduced in mms21-1 mutant. Conclusively, we provided evidence that AtMMS21 plays an important role in homologous recombination for DNA damage repair. (C) 2014 Elsevier B.V. All rights reserved.
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