期刊
DNA AND CELL BIOLOGY
卷 33, 期 3, 页码 128-135出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/dna.2013.2089
关键词
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资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education (MOE) [2013-053960]
- Leading Space Core Technology Development Program through NRF
- Ministry of Science, ICT&Future Planning (MSIP) [2013-042433]
- MSIP [2011-0030888]
Exposure to microgravity is supposed to affect almost all biological systems, and we speculated that microgravity is potentially involved in autophagy regulation. A clinostat was used to simulate microgravity, and HEK293 cells that stably express GFP-LC3 were used for sensitive monitoring of autophagy induction. The clinorotation of GFP-LC3 cells resulted in autophagosome formation in the cytoplasm and a change in autophagosomal marker expression. Autophagy induction was accompanied by phosphorylation of AMPK (Thr 172) and by the dephosphorylation of mammalian target of rapamycin. To elucidate the role of AMPK in microgravity-induced autophagy, we suppressed AMPK expression by knockdown via siRNA, which inhibited the induction of autophagy upon exposure to microgravity. In addition, the clinorotation of C2C12 myotube cells resulted in the enlarged and distinctive LC3 spots in the cytoplasm and AMPK activation. These results indicate that simulated microgravity possibly contributes to autophagy induction by regulating AMPK.
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