期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 102, 期 -, 页码 78-84出版社
ELSEVIER
DOI: 10.1016/j.jpba.2014.08.029
关键词
Near-infrared; Ultraviolet; Adsorption; Saponins; Quantification
资金
- National S&T Major Project of China [2012ZX09101201-003]
- Fundamental Research Funds for the Central Universities [2014FZA7017]
The step of enriching and purifying saponins by macroporous resin column chromatography is closely related to the safety and efficacy of Panax notoginseng products during their manufacturing processes. Adsorption process is one of the most critical unit operations within each chromatographic cycle. In order to understand the adsorption process directly, it is necessary to develop a rapid and precise method to monitor the adsorption process in real time. In this study, comparative evaluation of using near-infrared (NIR) spectra, ultraviolet (UV) spectra and fused spectra to monitor the adsorption process of P. notoginseng was conducted. The uninformative variable elimination by partial least squares (UVE-PLS) regression models were established for quantification of notoginsenoside R-1, ginsenoside Rg(1), ginsenoside Re, ginsenoside Rb-1 and ginsenoside Rd in effluents based on different spectra. There was a significant improvement provided by the models based on fused spectra. The results in this work were conducive to solving the problems about real-time quantitative analysis of saponins during P. notoginseng adsorption. The fusion method of NIR and UV spectra combined with UVE-PLS regression could be a promising strategy to real-time analyze the components, which are difficult to be quantified by individual spectroscopic technique. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据