期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 115, 期 -, 页码 225-235出版社
ELSEVIER
DOI: 10.1016/j.jpba.2015.06.036
关键词
NMR metabolomics; Mouse; Obesity; Urine
资金
- Grant Agency of the Czech Republic [GA13-14105S]
- Institute of Organic Chemistry and Biochemistry [RVO: 61388963]
- Operational Program Prague - Competitiveness [CZ.2.16/3.1.00/24023]
- Grant Agency of the Czech Technical University in Prague [SGS13/203/OHK3/3T/13]
- Ministry of Education, Youth and Sports of the Czech Republic [LO1509]
The mouse model of monosodium glutamate induced obesity was used to examine and consequently optimize the strategy for analysis of urine samples by NMR spectroscopy. A set of nineteen easily detectable metabolites typical in obesity-related studies was selected. The impact of urine collection protocol, choice of H-1 NMR pulse sequence, and finally the impact of the normalization method on the detected concentration of selected metabolites were investigated. We demonstrated the crucial effect of food intake and diurnal rhythms resulting in the choice of a 24-hour fasting collection protocol as the most convenient for tracking obesity-induced increased sensitivity to fasting. It was shown that the Carr-Purcell-Meiboom-Gill (CPMG) experiment is a better alternative to one-dimensional nuclear Overhauser enhancement spectroscopy (1D-NOESY) for NMR analysis of mouse urine due to its ability to filter undesirable signals of proteins naturally present in rodent urine. Normalization to total spectral area provided comparable outcomes as did normalization to creatinine or probabilistic quotient normalization in the CPMG-based model. The optimized approach was found to be beneficial mainly for low abundant metabolites rarely monitored due to their overlap by strong protein signals. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据