Article
Immunology
My Dang, Trung Cao, Ignacio Vasquez, Ahmed Hossain, Hajarooba Gnanagobal, Surendra Kumar, Jennifer R. Hall, Jennifer Monk, Danny Boyce, Jillian Westcott, Javier Santander
Summary: The study evaluated the efficacy of orally delivered V. anguillarum bacterin on lumpfish and found that oral immunization was ineffective, while intraperitoneal delivery provided protection against vibriosis. This indicates that orally administered bacterin did not reach deep lymphoid tissues, rendering oral immunization ineffective.
Article
Immunology
Jongwon Lim, Suhee Hong
Summary: This study aimed to identify molecular mechanisms affected by a combined vaccine against Aeromonas salmonicida and Vibrio anguillarum. The vaccine upregulated gene expression associated with adaptive immune response markers and triggered differential expression of hundreds of genes on different days. Gene ontology and Kyoto Encyclopedia of Genetic and Genomic analyses identified immune system-related pathways and processes affected by the vaccine. Protein-protein interaction analysis revealed TNF as the central player in immune-related responses at different time points.
Article
Fisheries
Na Yang, Fuhang Song, Steven W. Polyak, Jianguo Liu
Summary: The study identified that the resistance mechanism of the V. anguillarum mutant to actinonin is associated with bacterial respiration.
Article
Fisheries
Baozhi Kuang, Kun Xiao, Wenqi Wang, Feng You, Peng Xiao, Shiyong Chen
Summary: This study analyzed the immunological properties and efficacies of bivalent inactivated vaccines in salmon. The results showed that the antigens from the two pathogenic strains had adjuvant effects on each other's vaccines. The proportions of antigens in the combined vaccines had different effects on immune response and protection against the specific pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xiaoxu Qu, Xia Wang, Bo Liu, Min Chen, Junhao Ning, Haijun Liu, Guilong Liu, Xin Xu, Xiaotong Zhang, Kai Yu, He Xu, Xia Lu, Chunde Wang
Summary: In this study, hybrids between bay scallops and Peruvian scallops exhibited higher resistance to Vibrio bacteria, but the mechanism is unclear. The gene IFI44 was found to play an important role in the immune response to Vibrio in scallops. These findings suggest that IFI44 gene is involved in the higher resistance of hybrid scallops to Vibrio bacteria.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Mohd Hafiz Ngoo Muhamad-Sofie, Aslah Mohamad, Mohamad Azzam-Sayuti, Jing Yie Lee, Wan Haifa Haryani Wan Omar, Yong Kit Chin, Mohammad Noor Azmai Amal, Salleh Annas, Mohd Zamri-Saad, Md Yasin Ina-Salwany
Summary: This study evaluates the effectiveness of three live-attenuated Vibrio harveyi vaccines in providing immunological protection to zebrafish offspring against pathogenic Vibrio spp. The results show that freeze-dried and stale LAVh vaccines provide longer immunological protection, while the FKVh vaccine group fails to protect against V. alginolyticus. However, both vaccine groups display 100% immunity against pathogenic Vibrio spp. The freeze-dried LAVh vaccine confers maternal immune protection to offspring, has a longer duration of immunological protection and cross-protection, and a longer shelf life, making it a potential commercial option for preventing Vibrio spp. infection.
AQUACULTURE RESEARCH
(2023)
Article
Immunology
Qian Chen, Bangguo Ma, Mingzhe Xu, Huiwen Xu, Zimiao Yan, Fei Wang, Yiran Wang, Zitong Huang, Shuchang Yin, Yancui Zhao, Lei Wang, Hongyan Wu, Xiaoli Liu
Summary: In this study, exosomes were isolated from Vibrio harveyi and Vibrio anguillarum using a supercentrifugation method, and their protein compositions were analyzed. The exosomes of these two vibrios contained virulence factors and also played roles in important bacterial activities. Further experiments confirmed the involvement of exosomes in vibrio toxicity. This study provides a valuable proteome database for understanding the pathogenic mechanisms of vibrios from the exosome perspective.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Barbara Rossi, Maria Angeles Esteban, Jose Maria Garcia-Beltran, Giulia Giovagnoni, Alberto Cuesta, Andrea Piva, Ester Grilli
Summary: This study conducted a preliminary screening of the in vitro antimicrobial activity of organic acids and nature-identical compounds commonly used in aquaculture against Vibrio species, and found that certain active principles showed effective pathogen control. The results suggest that the use of these substances could offer promising perspectives for controlling Vibrio spp. load in aquaculture, making production more efficient and sustainable.
Article
Fisheries
Jongwon Lim, Yoonyoung Jang, Hyun-Ja Han, Suhee Hong
Summary: In this study, four strains of Vibrio anguillarum were isolated and found to have the same serotype, biochemical characteristics, and virulence factor genes. However, there were differences in haemolytic activity among these strains, with the less pathogenic strain displaying gamma-haemolytic activity and the virulent strains showing alpha-haemolytic activity and higher empA gene expression. The most virulent strain, V. anguillarum RTBHR, caused high mortality rates in rainbow trout and Coho salmon. A formalin-inactivated vaccine of V. anguillarum RTBHR successfully induced protective and specific immune responses in rainbow trout.
JOURNAL OF FISH DISEASES
(2023)
Article
Immunology
Peng Jin, Fei Sun, Qin Liu, Qiyao Wang, Yuanxing Zhang, Xiaohong Liu
Summary: Oral vaccination is a convenient method for disease protection in aquaculture by adding vaccines as part of fish diet, but low protective efficiency is a major problem. In this study, an oral inactivated vaccine based on a chitosan/aluminum adjuvant system was developed, inducing Th1 and Th2 cells mediated immune responses, with upregulated expression of IL-6, IL-12, IFNc and IgM genes as driver cytokines.
Article
Biochemistry & Molecular Biology
Quanxin Gao, Shaokui Yi, Jinping Luo, Qianqian Xing, Jiali Lv, Panhuang Wang, Cuihua Wang, Yang Li
Summary: By knocking out the FlaB gene of Vibrio anguillarum, this study found little impact on the induction of immune-related gene expression, and the mutant displayed similar flagella morphology and immune characteristics to the wild-type strain. This suggests that knockout of FlaB may promote the expression and function of other flagellin proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Fan Da, Xiaoju Wan, Guixiang Lin, Jichang Jian, Shuanghu Cai
Summary: In this study, the fliR deletion mutant Delta fliR of V.alginolyticus was constructed and evaluated, and the gene expression differences between wild-type and Delta fliR were analyzed by transcriptomics. Results showed that Delta fliR significantly affected cell motility and related pathways. Additionally, Delta fliR as a live attenuated vaccine demonstrated high protective efficacy and immune stimulation in groupers.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Soon Ho Lee, Bo Ram Beck, Seok-Hong Hwang, Seong Kyu Song
Summary: This study presented a novel anti-Vibrio feed vaccine that can protect olive flounder from multiple strains of Vibrio pathogens. By activating immune responses, the vaccine significantly increased survival rates and weight gain rates in fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Qianyu Ji, Junfei Ma, Shuying Wang, Qing Liu
Summary: In this study, the target antigen VP2309 of V. parahaemolyticus was identified and a live vector vaccine strain based on Listeria monocytogenes was successfully constructed, showing immune protection against V. parahaemolyticus infection in zebrafish.
Article
Virology
Ruoyun Zeng, Weiqiang Pan, Yifan Lin, Mincong Liang, Jiajie Fu, Shaoping Weng, Jianguo He, Changjun Guo
Summary: Global aquaculture production reached a record of 122.6 million tons in 2020, with a total value of 281.5 billion U.S. dollars (USD). However, approximately 10% of farmed aquatic animal production is lost due to various infectious diseases, resulting in more than 10 billion USD of economic waste every year. Immersion vaccines are emerging as the key technology for aquatic disease prevention and control. Here, a safe and efficacious candidate immersion vaccine strain (&UDelta;orf103r/tk) of infectious spleen and kidney necrosis virus (ISKNV), in which the orf103r and tk genes were knocked out by homologous recombination, is described.
JOURNAL OF VIROLOGY
(2023)