Article
Fisheries
Corbin J. Schuster, Katrina N. Murray, Justin L. Sanders, Michael L. Kent
Summary: Environmental DNA (eDNA) water assays are being used to detect important pathogens in confined aquaculture systems. A non-lethal assay has been developed to detect P. neurophilia in tank water, providing a valuable tool for routine surveillance in zebrafish research facilities.
Article
Fisheries
Corbin J. Schuster, Taylor G. Kreul, Colleen E. Al-Samarrie, James T. Peterson, Justin L. Sanders, Michael L. Kent
Summary: Pseudoloma neurophilia poses a critical threat to the zebrafish model. The study found that whole-body qPCR analysis can be a standardized diagnostic method and can detect the infection as early as 4 days post-exposure, while histology detection takes 92 days. The prevalence of infection reached 30%-40% (histology) or 40%-90% (PCR) at the end of the experiment.
JOURNAL OF FISH DISEASES
(2022)
Review
Immunology
Michal Stosik, Beata Tokarz-Deptula, Wieslaw Deptula
Summary: This review presents Danio rerio as a model organism for studying hematopoiesis in vertebrates and discusses the development of this process in fish and mammals, as well as the impact of microenvironmental factors.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Francesco Dalla Barba, Michela Soardi, Leila Mouhib, Giovanni Risato, Eylem Emek Akyurek, Tyrone Lucon-Xiccato, Martina Scano, Alberto Benetollo, Roberta Sacchetto, Isabelle Richard, Francesco Argenton, Cristiano Bertolucci, Marcello Carotti, Dorianna Sandona
Summary: Sarcoglycanopathies, or limb girdle muscular dystrophy 3-6, are rare muscular dystrophies that cause high disability, respiratory and cardiac problems. This study generated and characterized zebrafish models of these diseases using CRISPR/Cas9 genome editing, which showed progressive disease phenotypes. These zebrafish models will enhance our understanding of the disease and assist in drug screening and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Sinuo Tian, Sen Yan, Zhiyuan Meng, Shiran Huang, Wei Sun, Ming Jia, Miaomiao Teng, Zhiqiang Zhou, Wentao Zhu
Summary: The study found that exposure to BPA and TBBPA at commonly found concentrations in the environment can lead to hyperphagia and obesity in zebrafish, along with significant lipid accumulation in the liver. Furthermore, BPA and TBBPA increase appetite and induce obesity by activating the CB1 receptor.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Xin Chen, Wenhua Li
Summary: The study found that exposure to isoflucypram may cause developmental abnormalities, hatching delay, and decreased heart rates in zebrafish embryos, affecting the expression levels of cardiac-specific genes and erythropoiesis-related genes. Furthermore, most of the differentially expressed genes were enriched in heart development or hemopoiesis processes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Toshikazu Kuroda, Carolyn M. Ritchey, Christopher A. Podlesnik
Summary: Zebrafish show social behavior that is a result of complex and interdependent interactions among conspecifics. Previous research lacked clear evidence on how specific conspecific movements contribute to preference for social stimuli. This research examined the dependency between individual fish's motion and a social-stimulus fish's motions and found that experimental fish preferred dependent and interactive movements.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
A. C. N. Moraes, S. Shah, V. F. Magalhaes, H. R. Habibi
Summary: This study characterized the developmental effects of CYN using zebrafish larvae as a model organism. Exposure to CYN resulted in decreased growth, increased developmental abnormalities, and changes in gene expression related to thyroid function and oxidative stress. These results highlight the multiple effects of environmentally relevant CYN concentrations on zebrafish embryos.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Xueying Ma, Wenhua Li
Summary: This study evaluated the potential effects of the fungicide Amisulbrom (AML) on fish using a zebrafish model. Results showed that AML-treated zebrafish embryos exhibited severe developmental defects, including pericardial edema and decreased heart rates. Key marker genes associated with cardiovascular development were abnormally expressed in response to AML treatment, suggesting a specific impact on cardiovascular development by AML.
Article
Environmental Sciences
Giuseppe De Marco, Gea Oliveri Conti, Alessia Giannetto, Tiziana Cappello, Mariachiara Galati, Carmelo Iaria, Eloise Pulvirenti, Fabiano Capparucci, Angela Mauceri, Margherita Ferrante, Maria Maisano
Summary: In recent years, there has been increasing concern regarding the presence and accumulation of plastic debris in the environment, as well as the impact of microplastics (MPs) on biota. This study conducted a fish embryo toxicity test on zebrafish exposed to polystyrene MPs, revealing that the MPs had a moderate delay on hatching and caused deformities and compromised visual structure in larvae development. Additionally, increased gene transcription involved in oxidative stress and cellular detoxification were observed in the MPs-exposed zebrafish larvae.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Menglan Su, Rongkai Bao, Yaqing Wu, Bo Gao, Peng Xiao, Wenhua Li
Summary: The developmental toxicity and hepatotoxicity of diafenthiuron in zebrafish were investigated in this study. Results showed that diafenthiuron exposure significantly shortened the body lengths of zebrafish larvae and decreased superoxide dismutase activity. It also downregulated the spatiotemporal expression of pituitary development marker genes and liver-specific marker genes. These findings provide evidence of the detrimental effects of diafenthiuron on aquatic organisms and are important for environmental risk assessment.
Article
Marine & Freshwater Biology
Xin Chen, Tiantong Qiu, Peng Xiao, Wenhua Li
Summary: Isoflucypram has been found to have teratogenic effects on retinogenesis in zebrafish, causing abnormalities in retinal development and increased retinal cell apoptosis. This SDHI fungicide also demonstrates toxic effects on non-target aquatic organisms, highlighting the need for further investigation.
AQUATIC TOXICOLOGY
(2022)
Article
Environmental Sciences
Mingrui Yuan, Wenhua Li, Peng Xiao
Summary: In this study, exposure to BIX was found to induce cardiac toxicity in zebrafish embryos, leading to cardiac dysplasia and dysfunction. This toxicity was associated with decreased expression of erythropoiesis-related and cardiac development-related genes in response to BIX treatment. These findings highlight the potential environmental risks of BIX exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Xin Chen, Tiantong Qiu, Mengjun Pan, Peng Xiao, Wenhua Li
Summary: In this study, the influence of fluxapyroxad on hematopoietic development was evaluated using zebrafish embryos. The results showed that fluxapyroxad treatment led to abnormal distribution and decreased levels of hemoglobin, as well as reduced transcription levels of genes involved in erythropoiesis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Paritosh Mondal, Pallab Shaw, Arpan Dey Bhowmik, Arindam Bandyopadhyay, Muthammal Sudarshan, Anindita Chakraborty, Ansuman Chattopadhyay
Summary: This study found that the combined exposure of arsenic and fluoride had minimal impact on the female zebrafish brain, with lower oxidative stress response and tissue damage, indicating an antagonistic effect between the two toxicants.
Article
Microbiology
Steve Ndikumana, Adrian Pelin, Alexandre Williot, Justin L. Sanders, Michael Kent, Nicolas Corradi
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2017)
Article
Fisheries
S. Spagnoli, J. Sanders, M. L. Kent
JOURNAL OF FISH DISEASES
(2017)
Article
Developmental Biology
Justin L. Sanders, Virginia Watral, Mark F. Stidworthy, Michael L. Kent
Article
Developmental Biology
Justin L. Sanders, Joana F. Monteiro, Sandra Martins, Ana Catarina Certal, Michael L. Kent
Article
Parasitology
Douglas F. Markle, Andrew Janik, James T. Peterson, Anindo Choudhury, David C. Simon, Vasyl V. Tkach, Mark R. Terwilliger, Justin L. Sanders, Michael L. Kent
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2020)
Review
Fisheries
M. L. Kent, J. L. Sanders, S. Spagnoli, C. E. Al-Samarrie, K. N. Murray
JOURNAL OF FISH DISEASES
(2020)
Correction
Multidisciplinary Sciences
Martin McPhillie, Ying Zhou, Kamal El Bissati, Jitender Dubey, Hernan Lorenzi, Michael Capper, Amanda K. Lukens, Mark Hickman, Stephen Muench, Shiv Kumar Verma, Christopher R. Weber, Kelsey Wheeler, James Gordon, Justin Sanders, Hong Moulton, Kai Wang, Taek-Kyun Kim, Yuqing He, Tatiana Santos, Stuart Woods, Patty Lee, David Donkin, Eric Kim, Laura Fraczek, Joseph Lykins, Farida Esaa, Fatima Alibana-Clouser, Sarah Dovgin, Louis Weiss, Gael Brasseur, Dyann Wirth, Michael Kent, Leroy Hood, Brigitte Meunieur, Craig W. Roberts, S. Samar Hasnain, Svetlana V. Antonyuk, Colin Fishwick, Rima McLeod
SCIENTIFIC REPORTS
(2020)
Article
Fisheries
Lauren Norris, Noah Lawler, Andree Hunkapiller, Donna M. Mulrooney, Michael L. Kent, Justin L. Sanders
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Michael L. Kent, Katrina N. Murray, Maurine R. Hobbs, Louis M. Weiss, Sean T. Spagnoli, Justin L. Sanders
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Marion Jalenques, Justin Sanders, Lilian Tran, Laurie Beaupre, Michael Kent, Stephane Lair
Summary: This study reported for the first time the muscular infections by microsporidia in Arctic char, which were associated with digestive tract disorders in Inuit communities. The research showed a high prevalence of microsporidian infection in Arctic char populations, but with low pathogenicity and a correlation with gender and body condition of the fish.
DISEASES OF AQUATIC ORGANISMS
(2021)
Article
Fisheries
Travis Neal, Michael L. Kent, Justin Sanders, Carl B. Schreck, James T. Peterson
Summary: Research shows that Pacific salmon in lakes and reservoirs above dams are prone to infection with the ectoparasitic copepod Salmincola californiensis. Factors affecting parasite infection prevalence and intensity include water temperature, copepodid density, and fish size, with infected fish potentially being reinfected.
JOURNAL OF FISH DISEASES
(2021)
Article
Marine & Freshwater Biology
Thomas P. Hurst, Louise A. Copeman, Jessica F. Andrade, Michelle A. Stowell, Colleen E. Al-Samarrie, Justin L. Sanders, Michael L. Kent
Summary: The study found that walleye pollock larvae exposed to high CO2 levels had reduced swim bladder inflation rates, changes in lipid cycling, and higher survival rates, indicating potential sub-lethal effects. These findings emphasize the importance of evaluating the impacts of ocean acidification on marine fishes across a wide range of traits and life stages.
Article
Fisheries
Corbin J. Schuster, Taylor G. Kreul, Colleen E. Al-Samarrie, James T. Peterson, Justin L. Sanders, Michael L. Kent
Summary: Pseudoloma neurophilia poses a critical threat to the zebrafish model. The study found that whole-body qPCR analysis can be a standardized diagnostic method and can detect the infection as early as 4 days post-exposure, while histology detection takes 92 days. The prevalence of infection reached 30%-40% (histology) or 40%-90% (PCR) at the end of the experiment.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Christina A. Murphy, William Gerth, Travis Neal, Kelsi Antonelli, Justin L. Sanders, Trevor Williams, Ruben-Lee Roennfeldt, Rachel S. Crowhurst, Ivan Arismendi
Summary: This study explores the apparent infection of Salmincola californiensis, a parasite on Pacific salmon and trout. It was found that adult female copepods showed unusual coloration and similar color patterns were observed under laboratory settings. The cause of the apparent infection could not be identified, but both bacterial infection and iridovirus infection were considered plausible.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2023)
Article
Fisheries
Crystal L. Herron, Natassia E. Ruse, Daniel D. Rockey, Justin L. Sanders, James T. Peterson, Carl B. Schreck, Michael L. Kent
Summary: We provide evidence that the freshwater parasitic copepod, Salmincola californiensis, can act as a vector for the bacterium Aeromonas salmonicida. Through various experiments, we found that fish infected with copepods developed furunculosis caused by A. salmonicida. We also detected fluorescently labelled A. salmonicida in copepod egg sacs and successfully cultured the bacterium from egg sacs collected from infected adult Chinook salmon in a freshwater hatchery.
JOURNAL OF FISH DISEASES
(2023)