Article
Virology
Samantha R. Logan, Mark Seegobin, R. J. Neil Emery, Craig R. Brunetti
Summary: Viruses are parasites that depend on host cells to replicate and obtain resources for energy and macromolecule synthesis. This study assessed the impact of various purine compounds on the replication of frog virus 3 (FV3), an ecologically important iridovirus. The results showed that most purine compounds increased FV3 replication, with adenine and adenosine having the strongest effect. These findings provide insights into the role of purine metabolism during iridoviral infection.
Article
Biodiversity Conservation
J. Ruggeri, M. R. Pontes, L. P. Ribeiro, K. L. Gendreau, R. L. M. Sousa, L. F. Toledo
Summary: Emerging infectious diseases, such as amphibian chytridiomycosis caused by the chytrid fungus Batrachochytrium dendrobatidis and a viral disease caused by different ranaviruses, are causing declines in amphibian populations worldwide. A study in southern Brazilian Atlantic Forest found a higher prevalence of ranavirus infection compared to chytrid fungus infection in wild tadpoles. The presence of forest cover was positively correlated with ranavirus infection.
ANIMAL CONSERVATION
(2023)
Article
Fisheries
Alexander J. Douglas, Barbara A. Katzenback
Summary: Wood frogs, with their remarkable ability to tolerate whole-body freezing, are widely distributed in North America and the only amphibian found north of the Arctic Circle. However, recent mass mortalities in wild juvenile wood frogs caused by Ranavirus spp. highlight the serious threat posed to wood frog populations. Despite limited knowledge of the wood frog immune system and antiviral response, they are being increasingly studied as a model to understand anuran immune systems and antiviral responses.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Virology
Yun Tian, Francisco De Jesus Andino, Collins N. Khwatenge, Jiuyi Li, Jacques Robert, Yongming Sang
Summary: This study conducted a comprehensive genome-wide viral transcriptome profiling during FV3 infection across multiple amphibian host tissues. The findings suggest that Ranaviruses like FV3 have acquired previously unknown molecular mimics, interfering with host interferon signaling during evolution. Extensive analysis validated the expression of almost all of the 98 annotated ORFs and profiled their differential expression in a tissue-, virus-, and temporal class-dependent manner.
Article
Immunology
Yun Tian, Collins N. Khwatenge, Jiuyi Li, Francisco De Jesus Andino, Jacques Robert, Yongming Sang
Summary: Using a whole transcriptomic analysis, this study identified virus-specific reads in non-coding intergenic regions and multiple cis-regulatory elements (CREs) neighboring highly transcribed coding genes. Additionally, putative microRNA (miRNA) sequences targeting IFN-related genes were found in the FV3 genome, suggesting a complex interplay between viral and host gene expressions in response to host interferon (IFN) response.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Fei Ke, Qi-Ya Zhang
Summary: Genomic studies of viral diseases in aquaculture have gained increasing attention due to the potential for recombination, mutation, and insertion in the viral genome, which could lead to more severe diseases and widespread infections. This review focuses on aquaculture viruses belonging to Varidnaviria, Duplodnaviria, and Naldaviricetes, and three families: Iridoviridae, Alloherpesviridae, and Nimaviridae. These large DNA viruses with genomes larger than 100 kbp and more than 100 genes have been extensively studied for their replication, transcription, virus-host interactions, and genetic variation.
Article
Virology
Zhenlu Sun, Jinjie Xu, Peihua Niu, Miao Jin, Qiao Gao, Ruiqing Zhang, Ji Wang, Yong Zhang, Xuejun Ma
Summary: This study analysed the pathogenic spectrum and epidemiological characteristics of infectious diarrhea in Yantai City, China. The study found that rotavirus, norovirus, enteric adenoviruses, enterovirus universal, and astrovirus were the main pathogens. Infectious diarrhea in Yantai City is primarily viral and does not show a significant seasonal pattern.
Review
Microbiology
Marcia Cardoso, Izabela Ragan, Lindsay Hartson, Raymond P. P. Goodrich
Summary: Emerging infectious disease threats are increasing in frequency due to various societal, political, and geographical pressures. Blood transfusions, although essential in treating medical conditions, pose a potential risk of infectious disease transmission. Proactive pathogen reduction methods, such as the Mirasol PRT system, have been implemented globally to reduce the likelihood of disease transmission via transfusions. This review examines the effectiveness of the Mirasol PRT system in reducing the transmission of emerging threats such as Ebola, SARS-CoV-2, hepatitis E, mpox, and other agents.
Article
Fisheries
Natalie K. Stilwell, Salvatore Frasca, Lisa L. Farina, Kuttichantran Subramaniam, Kamonchai Imnoi, Pedro H. Viadanna, Lacey Hopper, Jeffrey Powell, James Colee, Thomas B. Waltzek
Summary: Ranaviruses, large double-stranded DNA viruses, are increasingly being detected among aquacultured and wild fishes. Ranavirus disease outbreaks have resulted in significant economic losses in sturgeon hatcheries. Temperature manipulation may be an effective strategy to minimize ranavirus-associated disease.
DISEASES OF AQUATIC ORGANISMS
(2022)
Review
Biotechnology & Applied Microbiology
Tharmala Tharmalingam, Xiaobing Han, Ashley Wozniak, Laura Saward
Summary: Passive immunization with polyclonal hyper immunoglobulin therapy provides immediate protection by transferring immunoglobulins, but requires maintaining an effective dose until a natural adaptive immune response or pathogen clearance occurs.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Alexandra Penafiel-Ricaurte, Stephen J. Price, William T. M. Leung, Mario Alvarado-Rybak, Andres Espinoza-Zambrano, Catalina Valdivia, Andrew A. Cunningham, Claudio Azat
Summary: A surveillance study was conducted to investigate the prevalence and distribution of ranavirus in amphibians and fish in central Chile. The results showed that out of the tested X. laevis populations, 9 individuals were found to be infected with ranavirus, while no other species tested positive. Phylogenetic analysis indicated that the detected ranavirus strains were identical to the type species FV3. The study suggests that X. laevis may act as a reservoir host and contribute to the local and global spread of ranavirus through invasion and the pet trade.
Review
Engineering, Multidisciplinary
Dayi Zhang, Yunfeng Yang, Miao Li, Yun Lu, Yi Liu, Jingkun Jiang, Ruiping Liu, Jianguo Liu, Xia Huang, Guanghe Li, Jiuhui Qu
Summary: The COVID-19 pandemic and concerns about other pandemics have attracted global attention. This review discusses the ecological barrier that affects the transmission of viruses from animals to humans. It explores the key factors influencing this barrier and analyzes the impact of human activities and climate change. The review provides important insights into protecting the ecological barrier and reducing the risks of emerging infectious diseases.
Article
Biodiversity Conservation
Joseph R. Hoyt, Katy L. Parise, John E. DePue, Heather M. Kaarakka, Jennifer A. Redell, William H. Scullon, Rich O'Reskie, Jeffrey T. Foster, A. Marm Kilpatrick, Kate E. Langwig, J. Paul White
Summary: Emerging infectious diseases have led to decreases in population and biodiversity. This study examined the effectiveness of managing environmental exposure to reduce the impacts of a specific disease in bats. The results showed that targeted environmental management can be an effective strategy for controlling wildlife diseases.
JOURNAL OF APPLIED ECOLOGY
(2023)
Article
Multidisciplinary Sciences
Jennifer Cable, Anthony Fauci, William E. Dowling, Stephan Guenther, Dennis A. Bente, Pragya Dhruv Yadav, Lawrence C. Madoff, Lin-Fa Wang, Rahul K. Arora, Maria Van Kerkhove, May C. Chu, Thomas Jaenisch, Jonathan H. Epstein, Simon David William Frost, Daniel G. Bausch, Lisa E. Hensley, Eric Bergeron, Ioannis Sitaras, Michael D. Gunn, Thomas W. Geisbert, Cesar Munoz-Fontela, Florian Krammer, Emmie de Wit, Pontus Nordenfelt, Erica Ollmann Saphire, Sarah C. Gilbert, Kizzmekia S. Corbett, Luis M. Branco, Sylvain Baize, Neeltje van Doremalen, Marco A. Krieger, Sue Ann Costa Clemens, Renske Hesselink, Dan Hartman
Summary: The importance of maintaining and strengthening investments in infrastructure, collaborations, laboratory and manufacturing capacity, diagnostics, etc. during the COVID-19 pandemic to enable quick, concerted responses to future threats, especially to zoonotic pathogens, was emphasized during a symposium gathering researchers from various sectors.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Ecology
Kate E. Langwig, J. Paul White, Katy L. Parise, Heather M. Kaarakka, Jennifer A. Redell, John E. DePue, William H. Scullon, Jeffrey T. Foster, A. Marm Kilpatrick, Joseph R. Hoyt
Summary: Emerging infectious diseases can have devastating effects on host communities, with the timing of novel pathogen arrival shaping the trajectory of epidemics. Bats are most likely to spread P. destructans when highly infectious but with reduced mobility, with the predominant spread pattern of the fungus occurring in midwinter. The consequences of varying pathogen arrival times have substantial effects on disease impacts within bat communities, highlighting the importance of accurate estimation of spread timing and arrival consequences.
JOURNAL OF ANIMAL ECOLOGY
(2021)
Article
Engineering, Environmental
Devin K. Jones, Kathryn A. Quinlin, Maggie A. Wigren, Youn J. Choi, Maria S. Sepulveda, Linda S. Lee, David L. Haskins, Guilherme R. Lotufo, Alan Kennedy, Lauren May, Ashley Harmon, Thomas Biber, Nicolas Melby, Michael K. Chanov, Michelle L. Hudson, Peter B. Key, Katy W. Chung, David W. Moore, Jamie G. Suski, Edward F. Wirth, Jason T. Hoverman
Summary: Researchers have found it crucial to further explore the potential toxic effects of PFAS-free AFFF as a substitute for PFAS-containing AFFF. The study reveals variations in the toxicity of different chemical formulations on aquatic species, with some formulas being more toxic than others.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
R. Wesley Flynn, Gary Hoover, Michael Iacchetta, Samuel Guffey, Chloe de Perre, Belinda Huerta, Weiming Li, Jason T. Hoverman, Linda Lee, Maria S. Sepulveda
Summary: Per- and polyfluoroalkyl substances (PFAS) are contaminants that can negatively affect the health and development of amphibians. The sensitivity to PFAS varies among species, with longer developmental species being more susceptible. In areas with high levels of PFAS contamination, certain species may experience sublethal effects.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Ecology
Turner S. DeBlieux, Jason T. Hoverman
Summary: The field of natural enemy ecology aims to study the interactions between predator-prey and host-pathogen, and their effects on community structure and pathogen transmission. This study found that different natural enemies resulted in unique community structures, with predators having the most significant impact. Additionally, predators were able to reduce pathogen transmission, with different predator species having varying effects.
Article
Engineering, Environmental
Melissa E. Lech, Youn Jeong Choi, Linda S. Lee, Marisol S. Sepulveda, Jason T. Hoverman
Summary: Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants that have adverse effects on health and development in organisms. This study found that PFAS mixtures increased the susceptibility of larval American bullfrogs to parasitic infections, and there may be antagonistic effects among different PFAS.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Tyler D. Hoskins, Elizabeth B. Allmon, R. Wesley Flynn, Linda S. Lee, Youn Choi, Jason T. Hoverman, Maria S. Sepulveda
Summary: This study compared the toxicities of perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS), and found that the mixture had different toxic effects compared to the individual chemicals. The mixture's toxicity exceeded expectations and had impacts on the body condition and length of metamorphosis of the tadpoles.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Ecology
Andrew P. Hopkins, Jason T. Hoverman
Summary: In this study, the acute toxicity of two widely used fungicides, chlorothalonil and pyraclostrobin, on six North American larval amphibian species was examined. The results showed that pyraclostrobin was approximately 3.5x more toxic than chlorothalonil, with estimated LC50 values ranging from 5-18 μg/L for pyraclostrobin and 15-50 μg/L for chlorothalonil. Salamanders were found to be 3x more sensitive to pyraclostrobin than anuran species and equally as sensitive to chlorothalonil. The estimated LC50 values fell within the expected environmental concentration, indicating potential direct mortality in these species. Additional research is needed to assess the overall risk of these fungicides to amphibians and their aquatic habitats.
Article
Ecology
D. Riley Rackliffe, Jason T. Hoverman
Summary: Studies have found that laboratory-derived estimates of tolerance to contaminants can predict survival in field conditions for sensitive aquatic macroinvertebrates.
Article
Multidisciplinary Sciences
Rebecca H. Hardman, Laura K. Reinert, Kelly J. Irwin, Kendall Oziminski, Louise Rollins-Smith, Debra L. Miller
Summary: Hellbenders (Cryptobranchus alleganiensis) in the United States have experienced declines, with the Ozark subspecies listed as federally endangered. The presence of the fungus Batrachochytrium salamandrivorans (Bsal) poses a new threat. Skin secretions from Hellbenders were found to inhibit the growth of Bsal more effectively than Batrachochytrium dendrobatidis (Bd).
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Wesley C. Sheley, Matthew J. Gray, Mark Q. Wilber, Carolyn Cray, E. Davis Carter, Debra L. Miller
Summary: This study investigated the pathophysiological effects and immune responses associated with Bsal chytridiomycosis in Taricha granulosa. The results showed that electrolyte imbalances and dehydration induced by damage to the epidermis played a major role in the pathogenesis of Bsal chytridiomycosis in this species. Additionally, Bsal-infected T. granulosa exhibited a systemic inflammatory response identified through alterations in complete blood counts and protein electrophoretograms.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Environmental Sciences
Zacharias Pandelides, Jason Conder, Youn Choi, Elizabeth Allmon, Tyler Hoskins, Linda Lee, Jason Hoverman, Maria Sepulveda
Summary: This critical review synthesizes information on the ecotoxicity of per- and polyfluoroalkyl substances (PFAS) to amphibians, providing recommendations for risk assessors. The study highlighted the most sensitive endpoint, body mass, and recommended screening levels for PFOS and PFOA. The review also provided screening levels for PFAS concentrations in an amphibian diet, tissue, and moss substrate.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Veterinary Sciences
Eliza Baker, Rebecca H. Hardman, William B. Sutton, Sherri Reinsch, Michael Freake, Emily Holder, Carlin Frost, Bradley Nissen, Emilly Nolan, Richard Gerhold, Debra Miller
Summary: Populations of eastern hellbenders have been declining due to anthropogenic habitat disturbance and sedimentation. The impact of parasite-associated morbidity and mortality on hellbender populations is not well understood. This study detected trypanosome infection in hellbenders from Virginia, with the first report of the partial 18s rRNA sequence from hellbender trypanosomes in North America. Further research is needed to understand trypanosome epidemiology and implications for hellbender health.
JOURNAL OF WILDLIFE DISEASES
(2023)
Article
Multidisciplinary Sciences
Matthew J. Gray, Edward Davis Carter, Jonah Piovia-Scott, J. Patrick W. Cusaac, Anna C. Peterson, Ross D. Whetstone, Andreas Hertz, Aura Y. Muniz-Torres, Molly C. Bletz, Douglas C. Woodhams, John M. Romansic, William B. Sutton, Wesley Sheley, Allan Pessier, Catherine D. McCusker, Mark Q. Wilber, Debra L. Miller
Summary: Batrachochytrium salamandrivorans (Bsal), a fungal pathogen of amphibians, is emerging in Europe and could potentially invade North America. Experimental studies suggest that amphibian biodiversity loss due to Bsal invasion is expected to be greatest in the Appalachian Region and along the West Coast of the United States.
NATURE COMMUNICATIONS
(2023)
Correction
Veterinary Sciences
Wesley C. Sheley, Matthew J. Gray, Mark Q. Wilber, Carolyn Cray, E. Davis Carter, Debra L. Miller
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Ecology
Spencer Catron, Sarah Roth, Francesca Zumpano, Jason Bintz, James A. Fordyce, Suzanne Lenhart, Debra L. Miller, Jeanette Wyneken
Summary: Loggerhead sea turtles (Caretta caretta), a threatened species, predominantly nest in Florida and contribute to a significant portion of the Atlantic Ocean loggerhead population. However, the ecological mechanisms underlying their population dynamics remain understudied. This study examines the relationship between air temperature and emergence success across multiple nesting seasons, providing insights into the potential impact of climate change on loggerhead populations.
ECOLOGICAL MODELLING
(2023)
Article
Veterinary Sciences
Rebecca H. Hardman, Sawsan Ammar, Richard Gerhold, William Sutton, E. Davis Carter, Shawn Snyder, Jeronimo da Silva Neto, Meredith Tarkington, Matthew J. Gray, Debra L. Miller
Summary: Wildlife diseases pose a significant threat to species conservation, and it is crucial to implement comprehensive disease response programs for better identification of these diseases. Moribund and dead eastern newts in a pond in middle Tennessee were found to be infected with ranavirus and a previously undescribed Eimeria sp. These findings emphasize the importance of histopathologic evaluation and provide guidance for future outbreak investigations.
JOURNAL OF WILDLIFE DISEASES
(2023)