Article
Cardiac & Cardiovascular Systems
Tatsuro Hitsumoto, Osamu Tsukamoto, Ken Matsuoka, Junjun Li, Li Liu, Yuki Kuramoto, Shuichiro Higo, Shou Ogawa, Noboru Fujino, Shohei Yoshida, Hidetaka Kioka, Hisakazu Kato, Hideyuki Hakui, Yuki Saito, Chisato Okamoto, Hijiri Inoue, Jo Hyejin, Kyoko Ueda, Takatsugu Segawa, Shunsuke Nishimura, Yoshihiro Asano, Hiroshi Asanuma, Akiyoshi Tani, Riyo Imamura, Shinsuke Komagawa, Toshio Kanai, Masayuki Takamura, Yasushi Sakata, Masafumi Kitakaze, Jun-ichi Haruta, Seiji Takashima
Summary: This study demonstrates that dysregulation of cMLCK contributes to the development of cardiac systolic dysfunction in humans, and restoring cMLCK activity could serve as a novel myotropic therapy for advanced systolic heart failure.
Article
Agriculture, Multidisciplinary
Juan Wang, Hui Zhao, Ke Lv, Wei Zhao, Ning Zhang, Fan Yang, Xiang Wen, Xiaohua Jiang, Jingrui Tian, Xinjuan Liu, Chi-Tang Ho, Shiming Li
Summary: Pterostilbene (PTE) protects intestinal epithelial barrier integrity through the NF-kappa B-MLCK/p-MLC signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Critical Care Medicine
Chao Qin, Yi Jiang, Xing Chen, Yingxue Bian, Yaoqi Wang, Keliang Xie, Yonghao Yu
Summary: The study showed that dexmedetomidine treatment mitigated burn-induced intestinal barrier injury by inhibiting the MLCK/p-MLC signaling pathways, reducing histological damage, enhancing tight junction protein expression, and decreasing inflammation levels.
Article
Agriculture, Multidisciplinary
Shuiping Liu, Jinyan Li, Weili Kang, Yun Li, Lei Ge, Dandan Liu, Yunhuan Liu, Kehe Huang
Summary: This study revealed that AFB1 induces intestinal barrier dysfunction through the FXR-mediated MLCK signaling pathway, providing novel insights into the prevention of mycotoxin poisoning in the intestine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mariam Anis, Janae Gonzales, Rachel Halstrom, Noman Baig, Cat Humpal, Regaina Demeritte, Yulia Epshtein, Jeffrey R. Jacobson, Dustin R. Fraidenburg
Summary: In this study, we investigated the role of nmMLCK in the proliferation and migration of pulmonary arterial endothelial cells in the pathogenesis of PAH. We found that increased nmMLCK activity, along with increased ERK expression, may contribute to the development of PAH by stimulating cellular proliferation and migration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Peripheral Vascular Disease
Masaaki Ito, Ryuji Okamoto, Hiromasa Ito, Ye Zhe, Kaoru Dohi
Summary: Regulation of muscle contraction is essential in the cardiovascular system, with the interaction between myosin and actin filaments being a common mechanism. The phosphorylation of myosin regulatory light chain plays a crucial role in smooth muscle contraction, while in cardiac muscle, troponin C is the primary target protein for Ca2+ with RLC phosphorylation also modulating contraction. Recent advances have improved our understanding of the regulatory role of RLC phosphorylation in smooth and cardiac muscles.
HYPERTENSION RESEARCH
(2022)
Article
Chemistry, Medicinal
Xuan Fang, Vladimir Bogdanov, Jonathan P. Davis, Peter M. Kekenes-Huskey
Summary: Calmodulin (CaM) is a protein that regulates cellular processes by using calcium as a signal. In this study, a molecular model of CaM-MLCK complex was constructed to explore the molecular mechanism of MLCK activation by CaM. The results provide insights into the structural basis and unique mechanisms of MLCK activation by different inhibitory CaM variants.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Oncology
Fan Wu, Hui Li, Heng Zhang, Yusheng Liao, Huanping Ren, Jie Wu, Dan Zheng
Summary: In a D-GalN/LPS-induced ALF model, TNF-alpha and IL-6 signaling may increase MLCK and ROCK expression levels, further mediate phosphorylation of MLC, resulting in tight junction dysregulation and intestinal barrier dysfunction.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biology
Liwen Du, Leilei Zhu, Xiaozhen Lu, Yuezhou Yu, Peng Liu, Jianneng Pan
Summary: Inhibiting myosin light chain kinase (MLCK) can improve heat stroke-induced intestinal injury in rats by reducing intestinal permeability and protecting the intestinal mucosal barrier. This study demonstrates that inhibiting the MLCK/p-MLC2 signaling pathway may serve as a novel therapeutic approach for heat stroke-induced intestinal damage.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Physiology
Dane W. Sorensen, Desirelys Carreon, James M. Williams, William J. Pearce
Summary: Chronic hypoxia followed by hypoxic culture induced translocation of MLCK among at least three subcellular fractions, potentially affecting contractility in fetal arteries.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY, INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Oncology
Jingyin Zhang, Yingjian Jiang, Hongbo Li, Jiang Wang, Chang Li, Dianliang Zhang
Summary: The study investigates the effect and mechanism of heme oxygenase-1 (HO-1) on intestinal barrier damage in severe acute pancreatitis (SAP). The results suggest that upregulation of HO-1 expression can attenuate SAP-induced intestinal mucosal barrier damage, which is achieved through the inhibition of MLCK/p-MLC signaling pathway.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Oncology
Yongjie Xu, Shengju Liu, Liying Zhu, Longguang Dai, Wen Qian, Jingzhi Zhang, Xing Li, Wei Pan
Summary: Green tea components can protect hippocampal neurons in diabetic rats from apoptosis by inhibiting the JNK/MLCK pathway, ultimately improving cognitive function.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemical Research Methods
Shibo Wang, Rui Wang, George Q. Li, Jun-Lae Cho, Yulin Deng, Yujuan Li
Summary: The study found that simulated microgravity for 21 days damaged the intestinal barrier, leading to decreased numbers of goblet cells, enlarged intercellular space, down-regulated adhesion molecules, and increased intestinal permeability. Proteomic analysis revealed that simulated microgravity significantly down-regulated adhesion molecules, deteriorated metabolic pathways, focal adhesion pathways, and the regulation of actin cytoskeleton pathways.
JOURNAL OF PROTEOMICS
(2021)
Article
Integrative & Complementary Medicine
Xuke Han, Yang Gao, Xuan Yin, Shengju Wang, Xiaoran Zhang, Qiu Chen
Summary: Electroacupuncture (EA) appears to be effective in ameliorating diabetic bladder dysfunction (DBD) by reducing bladder hypertrophy, repairing the mucosa layer, and reversing impairment in detrusor smooth muscle cells (SMCs) through regulating p-MLC and p-MLCK levels.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Environmental Sciences
Lianxin Li, Jinge Xin, Hesong Wang, Yadong Wang, Weiqi Peng, Ning Sun, Haonan Huang, Yanxi Zhou, Xingmei Liu, Yu Lin, Jing Fang, Bo Jing, Kangcheng Pan, Yan Zeng, Dong Zeng, Xiang Qin, Yang Bai, Xueqin Ni
Summary: Fluoride is a common contaminant that poses challenges to animal and human health. This study investigates the role of the cytoskeleton in fluoride-induced barrier dysfunction and provides potential therapeutic targets for fluoride-induced intestinal injury.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)