期刊
ACS NANO
卷 9, 期 3, 页码 2917-2925出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn506952g
关键词
fluorescence microscopy; quantum dots; super-resolution microscopy; stochastic optical reconstruction microscopy
类别
资金
- National Science Foundation [MCB1052672]
We demonstrate multicolor three-dimensional super-resolution imaging with quantum dots (QSTORM). By combining quantum dot asynchronous spectral blueing with stochastic optical reconstruction microscopy and adaptive optics, we achieve three-dimensional imaging with 24 nm lateral and 37 nm axial resolution. By pairing two short-pass filters with two appropriate quantum dots, we are able to image single blueing quantum dots on two channels simultaneously, enabling multicolor imaging with high photon counts.
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