Article
Oncology
Aileen I. Fernandez, Niki Gavrielatou, Leena McCann, Saba Shafi, Myrto K. Moutafi, Sandra Martinez-Morilla, Ioannis A. Vathiotis, Thazin Nwe Aung, Vesal Yaghoobi, Yalai Bai, Yvonne G. Chan, Jodi Weidler, Roy Herbst, Michael Bates, David L. Rimm
Summary: This study investigates the use of PD-L1 mRNA level as a predictive biomarker for nonresponse to immune checkpoint inhibitors (ICIs) in lung cancer patients. The results show that high levels of PD-L1 and PD-L2 mRNA are associated with improved long-term benefit and overall survival, while low PD-L1 mRNA level has a high negative predictive value for absence of long-term benefit.
JOURNAL OF THORACIC ONCOLOGY
(2022)
Article
Virology
Oivind Bergh, Torsten Snogdal Boutrup, Renate Johansen, Helle Frank Skall, Nina Sandlund, Niels Jorgen Olesen
Summary: The viral hemorrhagic septicaemia virus (VHSV) is shown to cause high mortalities in Atlantic herring. Two VHSV genotypes (Ib and III) from Norway and Denmark have been tested, and it is found that genotype Ib has a high prevalence and mortality rate, suggesting a negative impact on wild Atlantic herring stocks. Further research is needed to understand how VHSV affects wild Atlantic herring stocks.
Article
Infectious Diseases
Victor Hugo Brunaldi Marutani, Flavia Miyabe, Alice Fernandes Alfieri, Camila Domit, Andressa Maria Rorato Nascimento de Matos, Mario Roberto Castro Meira Filho, Ana Paula Frederico Rodrigues Loureiro Bracarense
Summary: Cetacean morbillivirus (CeMV) was found to have a prevalence of 27.5% in non-epizootic conditions in a world heritage site in Southern Brazil, with interstitial pneumonia and non-suppurative encephalitis as main pathological findings. The study used a combination of pathological, immunohistochemical, and molecular assays to investigate CeMV occurrence under non-epizootic circumstances, shedding light on the virus's behavior in such conditions.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Medicine, Research & Experimental
Yu Gao, Yiwei Wang, Rende Guo
Summary: This study found that the expression of NKD2 was associated with the progression and prognosis of thyroid carcinoma (THCA), suggesting it could be a potential biomarker and therapeutic target for THCA.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Medicine, General & Internal
Elodie Long-Mira, Alexandra Picard-Gauci, Sandra Lassalle, Veronique Hofman, Salome Lalvee, Virginie Tanga, Katia Zahaf, Christelle Bonnetaud, Virginie Lespinet, Olivier Camuzard, Henri Montaudie, Gilles Poissonnet, Thierry Passeron, Marius Ilie, Paul Hofman
Summary: Testing for the BRAF mutation is essential for managing patients with locally advanced or metastatic melanoma. Using molecular analysis based on DNA sequencing is the gold standard method for screening mutations. Comparison between the Idylla BRAF Mutation Test and anti-BRAF V600E immunohistochemistry showed a 91% agreement rate for the detection of BRAF V600E mutation.
Article
Oncology
Diana Marzic, Blazen Marijic, Tamara Braut, Stefan Janik, Manuela Avirovic, Ita Hadzisejdic, Filip Tudor, Katarina Radobuljac, Miran Coklo, Boban M. Erovic
Summary: IMP3 expression is higher in malignant laryngeal lesions compared to dysplasia and benign lesions, serving as a useful marker to differentiate between invasive and noninvasive lesions. Higher IMP3 expression is associated with worse clinical outcomes in patients with squamous cell carcinoma of the larynx.
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Biochemistry & Molecular Biology
Matilde Calderoni, Maddalena Altare, Luca Mastracci, Federica Grillo, Laura Cornara, Aldo Pagano
Summary: This study demonstrates a method for qualitative identification of Peganum harmala seeds using optical and electron microscopy, as well as the development of a real-time qPCR test that can detect Peganum harmala DNA in quantities as low as less than 1 pg. In addition to species specificity and high sensitivity, this method accurately quantifies the presence of Peganum harmala seeds or seed parts in complex herbal mixtures, providing an indication of the danger level of the product.
Article
Virology
Stephanie Jahne, Sandra Felten, Michele Bergmann, Katharina Erber, Kaspar Matiasek, Marina L. Meli, Regina Hofmann-Lehmann, Katrin Hartmann
Summary: This study aimed to detect FCoV and characterize S gene mutation profiles in cats. The results showed inconsistencies between commercial RT-qPCR and sequencing, and no mutated FCoV was confirmed.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Biochemistry & Molecular Biology
Carmen Diaz-Galindo, Denisse Calderon-Vallejo, Irma Hernandez-Jasso, Daniel Cervantes-Garcia, Diego Martinez-Diaz, David Ibarra-Martinez, Martin Munoz-Ortega, J. Luis Quintanar
Summary: The study analyzed the expression of gonadotrophin-releasing hormone receptor in the human spinal cord, finding it present in motoneurons and suggesting a potential extrapituitary role independent of the reproductive system.
NEUROCHEMICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Meng Wang, Tingting Ren, Prince Marowa, Haina Du, Zongchang Xu
Summary: This study identified stable reference genes PP2A and TUA5 in S. glauca under different salt concentrations and tissues, providing a reliable method for normalizing gene expression. These reference genes were validated in germinating seeds of S. glauca, highlighting their utility in accurate gene expression profiling.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Samia Hussein, Anan Fathi, Nehal S. Abouhashem, Samar Amer, Mohamed Hemeda, Hala Mosaad
Summary: The study revealed the clinical significance of SATB-1 and ERBB2 as potential biomarkers for predicting aggressive behavior and poor prognosis in bladder cancer patients. Additionally, a positive correlation between the expression of SATB-1 and ERBB2 was found along with a significant association with disease stage and lymph node involvement.
Article
Virology
Leonore Kuchler, Isabelle Rufli, Michel C. Koch, Melanie M. Hierweger, Ronja V. Kauer, Celine L. Boujon, Monika Hilbe, Anna Oevermann, Patrik Zanolari, Torsten Seuberlich, Corinne Gurtner
Summary: This report describes a case of polioencephalomyelitis in an 8-year-old alpaca, most likely of viral origin. The findings suggest the possibility of interspecies transmission of the astrovirus, BoAstV-CH13/NeuroS1.