Article
Microbiology
Christophe Le Terrier, Clement Viguier, Patrice Nordmann, Alejandro J. Vila, Laurent Poirel
Summary: Taniborbactam showed excellent activity against B1 MBLs, except for SIM-1. VIM-1-like enzymes, particularly VIM-83, were less inhibited by Taniborbactam compared to VIM-2-like.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Nouf Al-Rashed, Khalid M. Bindayna, Mohammad Shahid, Nermin Kamal Saeed, Abdullah Darwish, Ronni Mol Joji, Ali Al-Mahmeed
Summary: This study collected 50 carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from four main hospitals in Bahrain. The results showed that all isolates were resistant to imipenem, meropenem, and cephalosporins, and harbored various resistance genes. The study emphasizes the importance of strengthening surveillance and stringent infection control measures in clinical settings to prevent the further spread of such isolates.
Article
Chemistry, Medicinal
Yoshihiro Yamaguchi, Koichi Kato, Yoshimi Ichimaru, Wanchun Jin, Misa Sakai, Miki Abe, Jun-ichi Wachino, Yoshichika Arakawa, Yukina Miyagi, Masanori Imai, Nobuyuki Fukuishi, Yuriko Yamagata, Masami Otsuka, Mikako Fujita, Hiromasa Kurosaki
Summary: The study found that the mutation D120E caused a structural change in IMP-1, affecting its inhibitory activity. Citrate monobenzyl ester 1 was synthesized to evaluate the inhibitory activity, showing enhanced activity when a benzyl group was introduced.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Min-Ge Wang, Chang Fang, Kai-Di Liu, Lin-Lin Wang, Ruan-Yang Sun, Rong-Min Zhang, Liang-Xing Fang, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao
Summary: This study investigated the transmission and molecular characteristics of bla(NDM)-producing Escherichia coli between companion animals and their healthcare providers at veterinary clinics in Guangzhou, China. The study revealed the clonal spread of bla(NDM)-positive E. coli isolates between these two groups.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Sho Saito, Kayoko Hayakawa, Shinya Tsuzuki, Masahiro Ishikane, Maki Nagashima, Kazuhisa Mezaki, Yuko Sugiki, Taichi Tajima, Nobuaki Matsunaga, Satoshi Ide, Noriko Kinoshita, Yoshiki Kusama, Yumiko Fujitomo, Takato Nakamoto, Yuta Toda, Mitsuo Kaku, Eiichi N. Kodama, Norio Ohmagari
Summary: IMP-type carbapenemase, found in various Gram-negative bacteria, has been increasingly detected worldwide. This study found that IMP-CRE may not contribute to worsened clinical outcomes compared to CSE, and identified risk factors for CRE isolation including gastrostomy, previous intravenous therapy, hemodialysis, and broad-spectrum antimicrobial exposure. Fluoroquinolone and aminoglycosides were found to be potentially useful antibiotics for IMP-CRE infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Stefan E. Heiden, Katharina Sydow, Stephan Schaefer, Ingo Klempien, Veronika Balau, Peter Bauer, Nils-Olaf Huebner, Katharina Schaufler
Summary: The study characterized four VIM-positive isolates from northeastern Germany, revealing high levels of multidrug resistance and nearly identical VIM-1 resistance plasmids, which showed some similarity to publicly available plasmid sequences from other sources.
Article
Infectious Diseases
Souad Loqman, Nabila Soraa, Seydina M. Diene, Jean-Marc Rolain
Summary: The study investigated the occurrence of carbapenem-resistant Enterobacteriaceae (CRE) among hospitalized patients in Mohamed VI University Hospital in Marrakech, Morocco. It found various bacterial strains resistant to carbapenems, with Klebsiella pneumoniae being the most common species. The study also identified several carbapenemase-producing isolates, with blaOXA-48 genes being documented for the first time in Citrobacter freundii and Citrobacter braakii in Morocco.
Article
Microbiology
Kazuyoshi Gotoh, Makoto Miyoshi, I. Putu Bayu Mayura, Koji Iio, Osamu Matsushita, Fumio Otsuka, Hideharu Hagiya
Summary: The study found that Biapenem shows good in vitro efficacy against carbapenemase-producing Enterobacteriaceae, potentially serving as a new therapeutic option for infections caused by IMP-producing CPE.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuliang Zhou, Sulin Mi, Xin Rao, Qi Zhang, Shiwen Wei, Meng Xiao, Zhiyong Peng, Jing Wang
Summary: This study evaluated the role of individualized active surveillance using Xpert Carba-R in reducing the risk of carbapenem-resistant microorganisms (CRO) in ICU. The use of Xpert Carba-R for monitoring the presence of carbapenem resistance genes was associated with a lower CRO risk. Among the 3,765 patients admitted to Zhongnan Hospital of Wuhan University, 390 manifested the presence of CRO, indicating a prevalence of 10.36%. Individualized active surveillance using Xpert Carba-R may be effective in reducing the overall incidence of CRO in ICU.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Cecile Emeraud, Aba Mahamat, Agnes B. Jousset, Sandrine Bernabeu, Tania Goncalves, Camille Pommier, Delphine Girlich, Aurelien Birer, Christophe Rodriguez, Jean-Michel Pawlotsky, Thierry Naas, Remy A. Bonnin, Laurent Dorte
Summary: Since 2021, the rapid spread of highly resistant NDM-14 producing Klebsiella pneumoniae has been identified in hospitals in France. This strain, primarily imported from Morocco, is highly resistant to most antimicrobials and belongs to a single clone. However, it remains susceptible to colistin, cefiderocol, and the combination of aztreonam/avibactam.
Article
Infectious Diseases
P. Urbanowicz, R. Izdebski, A. Baraniak, D. Zabicka, W. Hryniewicz, M. Gniadkowski
Summary: This study identified the molecular and genomic background of the rapid increase of MPPA in Poland, with multi-clonal spread as the leading factor. Various novel and specific characteristics of MPPA were also revealed through the analysis.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Vincenzo Di Pilato, Giulia Errico, Monica Monaco, Tommaso Giani, Maria Del Grosso, Alberto Antonelli, Sophia David, Erika Lindh, Romina Camilli, David M. Aanensen, Gian Maria Rossolini, Annalisa Pantosti
Summary: A nationwide survey in Italy in 2016 revealed that while CG258 remained the most common CR-KP lineage, new high-risk lineages CG307, ST101, and ST395 have emerged. KPC remained the most common carbapenemase among the isolates. This study indicates a polyclonal population of CR-KP in Italy.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Aung-Hein Aung, Kala Kanagasabai, Jocelyn Koh, Pei-Yun Hon, Brenda Ang, David Lye, Swaine L. Chen, Angela Chow
Summary: The study found that CPE colonization was higher in acute-care hospitals compared to intermediate-term and long-term-care facilities within a healthcare network. Risk factors for CPE colonization varied between different types of facilities, highlighting the need for targeted screening and precautionary measures to address this issue. Genomic analysis revealed possible intra-hospital transmission but no evidence of transmission from hospitals to long-term care facilities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Medicine, General & Internal
Raouaa Maaroufi, Olfa Dziri, Linda Hadjadj, Seydina M. Diene, Jean-Marc Rolain, Chedly Chouchani
Summary: This study investigated the presence of Gram-negative bacteria in a hospital environment in Northwestern Tunisia, identifying various resistant strains and characterizing the resistance genes involved. Carbapenem resistance was detected in several isolates, with different resistance genes such as bla(NDM-1) and bla(CTX-M) being identified. Whole-genome sequencing of a P. aeruginosa strain revealed the presence of bla(OXA-494) and other resistance genes. This new epidemiological data highlights the need for new surveillance strategies and strict hygiene rules to control the spread of multidrug-resistant bacteria.
Article
Infectious Diseases
Melese Hailu Legese, Daniel Asrat, Abraham Aseffa, Badrul Hasan, Adane Mihret, Gote Swedberg
Summary: This study aimed to characterize ESBL and AmpC producing Enterobacteriaceae from sepsis patients. The results showed high genotypic frequencies of ESBL producing Enterobacteriaceae, with Hawassa having the highest detection rate. Several variants of ESBL and AmpC genes were detected.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)