Article
Multidisciplinary Sciences
Charlene Ranadheera, Greg J. German, Laura Steven, Dale Eung, Dmytro Lyubashenko, Jessica C. Pepin, Marko Zivcec, Kym Antonation, Cindi R. Corbett
Summary: This study evaluates the feasibility of using pooling patient specimens with the FilmArray Respiratory Panel 2.1 to increase testing capacity and maintain detection of SARS-CoV-2 and influenza virus. The results show that pooling samples has a high detection rate and positive agreement, and the sensitivity of the pooling assay meets the detection requirements for nucleic acid amplification testing.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Kirstine K. Sogaard, Vladimira Hinic, Daniel Goldenberger, Alexander Gensch, Michael Schweitzer, Veronika Battig, Martin Siegemund, Stefano Bassetti, Roland Bingisser, Michael Tamm, Manuel Battegay, Maja Weisser, Daiana Stolz, Nina Khanna, Adrian Egli
Summary: This study evaluated the diagnostic performance of the Biofire(©) FilmArray pneumonia (PN)-panel against standard culture in broncho-alveolar lavage (BAL) samples. The results showed that the PN-panel had high sensitivity and specificity, but low positive predictive value. The detection rate of bacterial pathogens was higher in the PN-panel compared to culture. Further evaluation is needed to assess the impact of the PN-panel on clinical management.
Article
Infectious Diseases
Nabil Gastli, Julien Loubinoux, Matthieu Daragon, Jean-Philippe Lavigne, Pierre Saint-Sardos, Helene Pailhories, Carole Lemarie, Hanaa Benmansour, Camille D'Humieres, Lauranne Broutin, Olivier Dauwalder, Michael Levy, Gabriel Auger, Solen Kerneis, Vincent Cattoir
Summary: The study evaluated the performance of FA-PP in detecting bacterial pathogens and antibiotic resistance genes in respiratory specimens, showing that it performed well in detecting pathogens in most specimens. The positive and negative percentage agreement values of FA-PP compared to culture were high, but there were some false-negative results. In addition, there was some discordance between the semi-quantification results of FA-PP and culture results.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Harshad Lade, Jung-Min Kim, Yousun Chung, Minje Han, Eun-Kyung Mo, Jae-Seok Kim
Summary: This study evaluated the performance of two multiplex nucleic acid amplification assays for simultaneous detection of multiple respiratory pathogens. The results showed consistent performance between the Allplex Respiratory Panel and the BioFire FilmArray, with some variations in detection rates for specific pathogens.
Article
Medicine, General & Internal
Maja Mijac, Suncanica Ljubin-Sternak, Irena Ivkovic-Jurekovic, Jasmina Vranes
Summary: Human bocavirus (HBoV) is an important respiratory pathogen, especially in children, but it is often found in co-detection with other respiratory viruses, which makes the diagnostic approach challenging. We compared multiplex PCR and quantitative PCR for HBoV with multiplex tandem PCR (MT-PCR) in 55 cases of co-detection of HBoV and other respiratory viruses. In addition, we investigated whether there is a connection between the severity of the disease, measured by the localization of the infection, and amount of virus detected in the respiratory secretions. No statistically significant difference was found, but children with large amount of HBoV and other respiratory virus had a longer stay in hospital.
Article
Medicine, General & Internal
Dolores Escudero, Jonathan Fernandez-Suarez, Lorena Forcelledo, Salvador Balboa, Javier Fernandez, Ivan Astola, Brigida Quindos, Rainer Campos, Fernando Vazquez, Jose Antonio Boga
Summary: In this study of COVID-19 patients, the Biofire FilmArray Pneumonia Panel Plus (FAPP+) demonstrated high sensitivity and negative predictive value in detecting pathogens, allowing for rapid and accurate microbiological diagnosis and optimization of antimicrobial treatment.
Article
Microbiology
Benjamin Berinson, Anna Both, Laura Berneking, Martin Christner, Marc Luetgehetmann, Martin Aepfelbacher, Holger Rohde
Summary: The BCID2 assay showed good clinical performance for species identification in positive blood cultures. It is a reliable tool for rapid blood culture processing and species identification, although further validation is needed for certain cases with discrepant results.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Cheng-Chun Tai, Cheng-Hsien Tsai, Yu-Han Huang, Chia-Lin Lee, Hsin-Pai Chen, Yu-Jiun Chan
Summary: Respiratory viruses, particularly NIRVs, were frequently detected in adults hospitalized with RTIs, showing distinctive seasonal variations. A substantial number of RVs were identified in lower RT specimens or from patients admitted to ICU, highlighting their important role in causing severe respiratory infections.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Biochemistry & Molecular Biology
Ying-Ju Chen, Tze-Kiong Er
Summary: This study examined the distribution of respiratory viral pathogens during the COVID-19 pandemic in an emergency department. The most common pathogen detected was rhinovirus/enterovirus, followed by adenovirus and SARS-CoV-2. The results showed perfect agreement between the cobas Liat system and the FilmArray TM RP for SARS-CoV-2 detection. The FilmArray TM RP assay is a reliable method for detecting SARS-CoV-2 and other respiratory infections.
Article
Multidisciplinary Sciences
Pablo Acera Mateos, Renzo F. Balboa, Simon Easteal, Eduardo Eyras, Hardip R. Patel
Summary: PACIFIC is a deep-learning algorithm that accurately detects SARS-CoV-2 and other common RNA respiratory viruses from patient RNA-seq data, with over 99% precision and recall. It can systematically monitor viral infections in the current global pandemic with accuracy in in vitro cell culture and patient datasets.
SCIENTIFIC REPORTS
(2021)
Review
Medical Laboratory Technology
Jing Yang, Dandan Li, Jie Wang, Rui Zhang, Jinming Li
Summary: Viral respiratory infections are common and serious, and early diagnosis is crucial for effective management. Real-time reverse transcriptase PCR (rRT-PCR) is the best method for early diagnosis, and multiplex rRT-PCR can address various challenges associated with viral respiratory infections. However, the design and validation of multiplex rRT-PCR are more complex, and comprehensive research is lacking. This review provides guidance for designing and optimizing multiplex rRT-PCR detection panels and assesses the clinical utility of multiplex rRT-PCR in viral respiratory tract infection diagnosis.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2022)
Article
Virology
Bengul Durmaz, Olkar Abdulmajed, Riza Durmaz, Mehmet Koroglu, Oguz Ari, Serdal Celik, Mahmut Tayyar Kalcioglu
Summary: The study found that at least one respiratory virus was detected in 56% of children with otitis media with effusion, compared to 12.8% in individuals without middle ear disease. There was no positive association between viruses and bacteria found in the middle ear cavity. The detection rate of respiratory viruses was significantly higher in children aged 6 to 11 years.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Xin Zhang, Colleen Knoth, Anh Pham, Amorce Lima, Rosario Dominguez, Irvin Ibarra-Flores, Juan C. Lopez, Dominic Uy, Suzane Silbert, Anami Patel, Michael Aye, Yi-Wei Tang, Jennifer Dien Bard
Summary: Early detection of respiratory tract pathogens is crucial for clinical management. The BioCode Respiratory Pathogen Panel utilizes RT-PCR and barcoded magnetic beads to detect and identify 17 pathogens. A multicenter clinical trial showed comparable performance between BioCode RPP and FilmArray Respiratory Panel. This high-throughput test offers automated testing capability and a sample-to-result time under 5 hours.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Ann R. Falsey, Angela R. Branche, Daniel P. Croft, Maria A. Formica, Michael R. Peasley, Edward E. Walsh
Summary: The BioFire FilmArray Pneumonia Panel (BioFire PN) is a useful tool for diagnosing lower respiratory tract infections by accurately identifying bacterial and viral pathogens, as well as atypical bacteria.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Johan Lindstrom, Kristina Elfving, Magnus Lindh, Johan Westin, Marie Studahl
Summary: Rapid syndromic panel testing, like the FilmArray ME panel, shows good performance in diagnosing central nervous system infections. However, the risk of false-negative results for specific pathogens (such as HSV-1) highlights the need for additional testing. Variability in performance among pathogens emphasizes the importance of ensuring accuracy through supplementary diagnostic procedures.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Letter
Dermatology
Paola Zelini, Eugenio Isoletta, Martina Volonte, Daniele Lilleri, Irene Cassaniti, Valeria Musella, Catherine Klersy, Fausto Baldanti, Valeria Brazzelli
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Virology
Cristina Galli, Laura Pellegrinelli, Federica Giardina, Guglielmo Ferrari, Sara Colonia Uceda Renteria, Federica Novazzi, Elisa Masi, Elisabetta Pagani, Giulia Piccirilli, Maria Vittoria Mauro, Sandro Binda, Benedetta Corvaro, Claudia Tiberio, Eleonora Lalle, Fabrizio Maggi, Cristina Russo, Stefania Ranno, Elisa Vian, Elena Pariani, Fausto Baldanti, Antonio Piralla
Summary: This study aimed to assess the circulation and molecular evolution of influenza viruses (IVs), particularly A(H3N2), during the 2021-2022 season in Italy. Analysis of respiratory specimens collected from January 1, 2022 to May 31, 2022 showed that all confirmed IVs were type A, with a majority being H3N2. Phylogenetic analysis revealed genetic clusters and mutations in the HA gene sequences, suggesting the presence of drifted strains. The study highlights the importance of molecular surveillance in assessing vaccine effectiveness and detecting novel strains.
Article
Immunology
Filippo Bianchini, Virginia Crivelli, Morgan E. Abernathy, Concetta Guerra, Martin Palus, Jonathan Muri, Harold Marcotte, Antonio Piralla, Mattia Pedotti, Raoul De Gasparo, Luca Simonelli, Milos Matkovic, Chiara Toscano, Maira Biggiogero, Veronica Calvaruso, Pavel Svoboda, Tomas Cervantes Rincon, Tommaso Fava, Lucie Podesvova, Akanksha A. Shanbhag, Andrea Celoria, Jacopo Sgrignani, Michal Stefanik, Vaclav Honig, Veronika Pranclova, Tereza Michalcikova, Jan Prochazka, Giuditta Guerrini, Dora Mehn, Annalisa Clabbattini, Hassan Abolhassani, David Jarrossay, Mariagrazia Uguccioni, Donata Medaglini, Qiang Pan-Hammarstroem, Luigi Calzolai, Daniel Fernandez, Fausto Baldanti, Alessandra Franzetti-Pellanda, Christian Garzoni, Radislav Sedlacek, Daniel Ruzek, Luca Varani, Andrea Cavalli, Christopher O. Barnes, Davide F. Robbiani
Summary: Using coldspot-guided antibody discovery, neutralizing antibodies to highly conserved viral epitopes were identified. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1 and synergizes with antibody rbd.042 for neutralization.
SCIENCE IMMUNOLOGY
(2023)
Article
Infectious Diseases
Cristina Merla, Irene Mileto, Stefano Gaiarsa, Cristian Achille, Stefano Ghirardello, Marta Corbella, Fausto Baldanti, Patrizia Cambieri
Summary: We report the isolation of a strain of Pantoea genus carrying a VIM carbapenemase, which is the first of its kind. The strain was obtained from a rectal swab of a 10-day-old newborn in a neonatal intensive care unit (NICU). Whole-genome sequencing revealed that the strain was identified as Pantoea brenneri. The strain harbored the carbapenemase gene bla(VIM-1). Immediate contact measures and isolation of the newborn prevented the dissemination of VIM-producing P. brenneri and the plasmid carrying the VIM-1 gene to other newborns.
Review
Virology
Angioletta Lasagna, Antonio Piralla, Sabrina Borgetto, Mattia Quaccini, Fausto Baldanti, Paolo Pedrazzoli
Summary: Torque teno virus (TTV) can be detected in over 90% of healthy individuals. It can be detected in various samples such as blood, serum, breast milk, cerebrospinal fluid, and saliva, as well as in different tissues such as the vagina and hepatocytes. TTV DNAemia is stable in healthy infected individuals, while TTV viral load is inversely correlated with T lymphocyte numbers and functions in immunocompromised populations. The role of TTV in cancer pathogenesis and response to anticancer treatments is still not well-known.
Article
Genetics & Heredity
Daniela Matuozzo, Estelle Talouarn, Astrid Marchal, Peng Zhang, Jeremy Manry, Yoann Seeleuthner, Yu Zhang, Alexandre Bolze, Matthieu Chaldebas, Baptiste Milisavljevic, Adrian Gervais, Paul Bastard, Takaki Asano, Lucy Bizien, Federica Barzaghi, Hassan Abolhassani, Ahmad Abou Tayoun, Alessandro Aiuti, Ilad Alavi Darazam, Luis M. Allende, Rebeca Alonso-Arias, Andres Augusto Arias, Gokhan Aytekin, Peter Bergman, Simone Bondesan, Yenan T. Bryceson, Ingrid G. Bustos, Oscar Cabrera-Marante, Sheila Carcel, Paola Carrera, Giorgio Casari, Khalil Chaibi, Roger Colobran, Antonio Condino-Neto, Laura E. Covill, Ottavia M. Delmonte, Loubna El Zein, Carlos Flores, Peter K. Gregersen, Marta Gut, Filomeen Haerynck, Rabih Halwani, Selda Hancerli, Lennart Hammarstroem, Nevin Hatipoglu, Adem Karbuz, Sevgi Keles, Christele Kyheng, Rafael Leon-Lopez, Jose Luis Franco, Davood Mansouri, Javier Martinez-Picado, Ozge Metin Akcan, Isabelle Migeotte, Pierre-Emmanuel Morange, Guillaume Morelle, Andrea Martin-Nalda, Giuseppe Novelli, Antonio Novelli, Tayfun Ozcelik, Figen Palabiyik, Qiang Pan-Hammarstroem, Rebeca Perez de Diego, Laura Planas-Serra, Daniel E. Pleguezuelo, Carolina Prando, Aurora Pujol, Luis Felipe Reyes, Jacques G. Riviere, Carlos Rodriguez-Gallego, Julian Rojas, Patrizia Rovere-Querini, Agatha Schlueter, Mohammad Shahrooei, Ali Sobh, Pere Soler-Palacin, Yacine Tandjaoui-Lambiotte, Imran Tipu, Cristina Tresoldi, Jesus Troya, Diederik van de Beek, Mayana Zatz, Pawel Zawadzki, Saleh Zaid Al-Muhsen, Mohammed Faraj Alosaimi, Fahad M. Alsohime, Hagit Baris-Feldman, Manish J. Butte, Stefan N. Constantinescu, Megan A. Cooper, Clifton L. Dalgard, Jacques Fellay, James R. Heath, Yu-Lung Lau, Richard P. Lifton, Tom Maniatis, Trine H. Mogensen, Horst von Bernuth, Alban Lermine, Michel Vidaud, Anne Boland, Jean-Francois Deleuze, Robert Nussbaum, Amanda Kahn-Kirby, France Mentre, Sarah Tubiana, Guy Gorochov, Florence Tubach, Pierre Hausfater, C. O. V. I. D. Human Genetic Effort, Isabelle Meyts, Shen-Ying Zhang, Anne Puel, Luigi D. Notarangelo, Stephanie Boisson-Dupuis, Helen C. Su, Bertrand Boisson, Emmanuelle Jouanguy, Jean-Laurent Casanova, Qian Zhang, Laurent Abel, Aurelie Cobat
Summary: Through a genome-wide rare variant burden association analysis, it was found that there is an association between at-risk variants in the TLR7 gene and rare loss-of-function variants in TLR3-dependent type I interferon immunity genes. These findings suggest that rare variants in TLR3- and TLR7-dependent type I interferon immunity genes may underlie life-threatening COVID-19 in patients under 60 years old.
Article
Public, Environmental & Occupational Health
Jose Camilla Sammartino, Marta Colaneri, Cecilia Bassoli, Mariaelena Ceresini, Antonio Piralla, Alessandro Ferrari, Elena Percivalle, Fausto Baldanti, Raffaele Bruno, Mario U. Mondelli
Summary: This study aimed to verify the presence of viable virus on different surfaces exposed to droplets released by coughing in SARS-CoV-2 RNA positive patients. The results showed that droplets on common inanimate surfaces did not contain viable virus, while laboratory-propagated viruses could survive and grow on the same surfaces.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Immunology
Marta Colaneri, Raffaella Lissandrin, Matteo Calia, Cecilia Bassoli, Elena Seminari, Alessandro Pavesi, Francesca Rovida, Fausto Baldanti, Alba Muzzi, Guido Chichino, Angelo Regazzetti, Cecilia Grecchi, Angelo Pan, Matteo Lupi, Erica Franceschini, Cristina Mussini, Raffaele Bruno
Summary: This study aimed to assess the efficacy of steroid therapy in reducing intrahospital mortality, length of stay, and neurological sequelae in WNND patients. The results showed that steroid therapy did not significantly reduce intrahospital mortality or neurological sequelae. More prospective data are needed to demonstrate the protective effect of steroid treatment.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Eun Kim, Muhammad S. Khan, Alessandro Ferrari, Shaohua Huang, Jose C. Sammartino, Elena Percivalle, Thomas W. Kenniston, Irene Cassaniti, Fausto Baldanti, Andrea Gambotto
Summary: The study evaluates the booster effect of an S1 subunit vaccine in aged mice previously primed with adenoviral vaccines. It demonstrates that a booster dose of the rS1Beta subunit vaccine, given to mice, induces strong and long-lived S1-specific immune responses and significantly increases neutralizing antibodies against Wuhan, Beta, and Delta strains, including cross-reactive antibodies against Omicron variants. The findings suggest that the rS1Beta subunit vaccine can offer cross-neutralization against broad variants, providing important implications for controlling breakthrough SARS-CoV-2 variants in elderly individuals primed with adenovirus-based vaccines.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Valentina Zuccaro, Greta Petazzoni, Irene Mileto, Marta Corbella, Erika Asperges, Paolo Sacchi, Sara Rattotti, Marzia Varettoni, Irene Defrancesco, Patrizia Cambieri, Fausto Baldanti, Luca Arcaini, Raffaele Bruno
Summary: Several studies have shown a strong connection between gut microbiota and the response to immunotherapy in tumor patients, suggesting that gut microbiota can serve as a biomarker for response. This study aimed to compare the gut microbiota diversity in chronic lymphocytic leukemia patients treated with B-cell receptor inhibitors for at least 12 months. The findings revealed differences in bacterial distribution between different response groups.
Article
Microbiology
Paola Zelini, Piera d'Angelo, Federica Zavaglio, Ehsan Soleymaninejadian, Liliana Mariani, Francesca Perotti, Mattia Dominoni, Stelvio Tonello, Pierpaolo Sainaghi, Rosalba Minisini, Daria Apostolo, Daniele Lilleri, Arsenio Spinillo, Fausto Baldanti
Summary: In this prospective study, it was found that vaccinated pregnant women and their newborns had higher levels of cytokines and immune responses after SARS-CoV-2 infection. Additionally, the newborns of vaccinated mothers had higher levels of specific antibodies. Vaccination can enhance the immune profile of pregnant women and provide immune protection to newborns.
Article
Infectious Diseases
Irene Mileto, Greta Petazzoni, Marta Corbella, Stefano Gaiarsa, Cristina Merla, Angela Kuka, Marina Ramus, Cristina Terulla, Micaela Brandolini, Antonio Piralla, Patrizia Cambieri, Fausto Baldanti
Summary: This article describes a new NDM-producing Kp clone, ST6668, that rapidly spread across hospitals in the Pavia province of Italy. Genomic surveillance programs are shown to be useful for tracking the spread of new clones with reduced antibiotic susceptibility.
Article
Immunology
Simona Secondino, Paolo Pedrazzoli, Sabrina Basso, Paolo Bossi, Alba Bianco, Ilaria Imarisio, Anna Pagani, Marica De Cicco, Stella Muscianisi, Michela Casanova, Carlo Morosi, Cristiana Bergamini, Marco Benazzo, Maria Cossu Rocca, Cesare Perotti, Fausto Baldanti, Marco Zecca, Lisa F. Licitra, Patrizia Comoli
Summary: This study aimed to explore the efficacy of autologous EBV-specific CTL transplantation in the treatment of recurrent/metastatic EBV-NPC patients after chemotherapy. The results showed that patients who received CTL transplantation had a 5-year overall survival rate of 44% and a median overall survival of 60 months. This indicates that autologous CTL transplantation can effectively control the disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Angioletta Lasagna, Irene Cassaniti, Francesca Arena, Federica Bergami, Elena Percivalle, Giuditta Comolli, Antonella Sarasini, Alessandro Ferrari, Daniela Cicognini, Roberta Schiavo, Giuliana Lo Cascio, Paolo Pedrazzoli, Fausto Baldanti
Summary: We prospectively evaluated the long-term immunogenicity of the third dose of the SARS-CoV-2 BNT162b2 mRNA vaccine in 55 patients undergoing cancer treatment. Our findings indicate that one year after receiving the third dose, more than 67% of patients showed an immune response against the virus, with less than 20% of patients showing no response to certain variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
