Article
Gastroenterology & Hepatology
Heiner Wedemeyer, Peter Erren, Uwe Naumann, Ansgar Rieke, Albrecht Stoehr, Tim Zimmermann, Kristina Lohmann, Bettina Koenig, Stefan Mauss
Summary: This study evaluated the real-world safety and effectiveness of Glecaprevir/pibrentasvir treatment in patients with cirrhosis from the German Hepatitis C-Registry. The results showed that Glecaprevir/pibrentasvir was well-tolerated and highly effective in patients with chronic hepatitis C and cirrhosis in real-world practice.
LIVER INTERNATIONAL
(2021)
Review
Medicine, General & Internal
Stefano Gitto, Carmela Cursaro, Alessandra Bartoli, Marzia Margotti, Pietro Andreone
Summary: Hepatitis C virus is a global health issue with direct-acting antivirals showing promising results in treatment. Despite the positive impacts, there are debated issues regarding the effects of treatment on various aspects of health. Identifying barriers to Hepatitis C virus care delivery is a complex challenge for achieving global eradication goals by 2030.
Article
Gastroenterology & Hepatology
Yu Jun Wong, Prem Harichander Thurairajah, Rahul Kumar, Kwong Ming Fock, Ngai Moh Law, Sin-Yoong Chong, Fria Gloriba Manejero, Tiing-Leong Ang, Eng Kiong Teo, Jessica Tan
Summary: This retrospective study in incarcerated HCV-infected patients in Singapore showed that unrestricted access to DAA significantly increased treatment access, completion rate, and reduced treatment default rate, highlighting the impact of this strategy in HCV micro-elimination efforts.
CLINICAL AND MOLECULAR HEPATOLOGY
(2021)
Review
Gastroenterology & Hepatology
Saleh A. Alqahtani, Massimo G. Colombo
Summary: The prevalence and burden of hepatitis C virus (HCV) in children are poorly understood. Vertical transmission and injection drug use are the main modes of infection. Early diagnosis and treatment are crucial in preventing HCV-induced severe liver disease. Direct-acting antiviral drugs have shown to be as safe and effective in young HCV patients as in adults.
LIVER INTERNATIONAL
(2021)
Article
Virology
Rajneesh Kumar, Kwai Peng Chan, Victoria Sze Min Ekstrom, Judith Chui Ching Wong, Kun Lee Lim, Wee Ching Ng, Shi Min Woo, Kian Sing Chan, Sobhana Thangaraju, Terence Yi Shern Kee, Sheryl Shien Wen Gan, Marjorie Wai Yin Foo, Lynette Lin Ean Oon, Wan Cheng Chow
Summary: HCV antigen testing shows high clinical sensitivity and specificity for the diagnosis of acute and chronic hepatitis C in at-risk and immunocompromised patients, with advantages of short turnaround time and relatively low cost for monitoring HCV infection and reinfection in patients on hemodialysis.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Ahmed K. Oraby, Cassandra L. Gardner, Robert F. Needle, Hassan M. Kofahi, Kylie R. Everard, Nathan G. A. Taylor, Suzette G. Rutihinda, Jacqueline P. Barry, Kensuke Hirasawa, Paris E. Georghiou, Rodney S. Russell
Summary: The novel small-molecule compound AO13 demonstrated a consistent but low-level antiviral effect against HCV, potentially acting on a late stage in the viral life cycle. This compound could serve as a lead compound for future drug development against other important viruses.
MICROBIOLOGY SPECTRUM
(2021)
Article
Medicine, General & Internal
Diana Valutite, Yulia Ostankova, Alexandr Semenov, Liudmila Lyalina, Areg Totolian
Summary: This study investigated the presence of primary resistance mutations in the NS3 and NS5 regions of HCV in treatment-naive patients. According to the results, primary mutations associated with resistance were found, which may increase the risk of treatment failure in the future.
Review
Virology
Christopher Dietz, Benjamin Maasoumy
Summary: Hepatitis C virus infection poses a significant threat to human health, but the development of specific antiviral drugs has revolutionized the treatment and brought hope to patients.
Review
Medicine, General & Internal
Elena Treppo, Luca Quartuccio, Gaafar Ragab, Salvatore De Vita
Summary: HCV infection can lead to rheumatologic manifestations such as mixed cryoglobulinemia, which may progress to cryoglobulinemic vasculitis and non-Hodgkin lymphoma. Antiviral therapy is essential to eradicate HCV, while immunosuppressive therapy can be used for organ-threatening manifestations.
Review
Gastroenterology & Hepatology
Omar Alshuwaykh, Paul Y. Kwo
Summary: Chronic hepatitis C infection is a major cause of liver disease and hepatocellular carcinoma globally. While treatment has been successful in the past, challenges in diagnosis and treatment remain, making global elimination of hepatitis C problematic. Multiple populations are being targeted for care models and efforts for a vaccine continue.
CLINICAL AND MOLECULAR HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Po-Yao Hsu, Yu-Ju Wei, Jia-Jung Lee, Sheng-Wen Niu, Jiun-Chi Huang, Cheng-Ting Hsu, Tyng-Yuan Jang, Ming-Lun Yeh, Ching- Huang, Po-Cheng Liang, Yi-Hung Lin, Ming-Yen Hsieh, Meng-Hsuan Hsieh, Szu-Chia Chen, Chia-Yen Dai, Zu-Yau Lin, Shinn-Cherng Chen, Jee-Fu Huang, Jer-Ming Chang, Shang-Jyh Hwang, Wan-Long Chuang, Chung-Feng Huang, Yi-Wen Chiu, Ming-Lung Yu
Summary: HCV-viremic patients on hemodialysis have a high prevalence of comedications with various potential drug-drug interactions (DDIs) with available DAA regimens. Elbasvir/grazoprevir has the fewest potential DDIs, while sofosbuvir/velpatasvir/voxilaprevir has the most potential DDIs among the analyzed regimens.
CLINICAL AND MOLECULAR HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Shogo Tanaka, Hiroji Shinkawa, Akihiro Tamori, Shigekazu Takemura, Sawako Uchida-Kobayashi, Ryosuke Amano, Kenjiro Kimura, Go Ohira, Kohei Nishio, Jun Tauchi, Masahiko Kinoshita, Norifumi Kawada, Shoji Kubo
Summary: A study examined the effects of direct-acting antiviral (DAA)-induced sustained virological response (SVR) in patients with hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC) after liver resection. While DAA-induced SVR itself may not suppress postoperative recurrence, the study found that DAA treatment improved liver function and prolonged postoperative survival in patients.
HEPATOLOGY RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Huan-Keat Chan, Mohamed Azmi Hassali, Noor Syahireen Mohammed, Azlina Azlan, Muhammad Radzi Abu Hassan
Summary: This study examined the barriers to scaling up HCV treatment in Malaysia and identified a range of supply- and demand-side issues, calling for strengthened inter-organizational collaborations to overcome these barriers.
Article
Infectious Diseases
Bingqin Tan, Mei Liu, Liming Wang, Jinhuan Wang, Fang Xiong, Xuli Bao, Yao Gao, Lele Yu, Jun Lu
Summary: The study suggests that the dynamic fluctuations of serum microRNAs could predict the efficacy of antiviral therapy for CHB. There were differences in the expression levels of microRNAs between responder and non-responder groups at baseline and after therapy, with some microRNAs correlated with hepatitis B virus DNA titer and e antigen.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Medicine, Research & Experimental
Jose Gabriel Solis, Ervin Saul Enciso Lopez, Aleida Bautista Santos, Juan Carlos Anda Garay, Jose Luis Calixto Rodriguez, Rosalba Moreno Alcantar, Luis Montiel Lopez
Summary: The study aimed to evaluate the response of carotid atherosclerosis to treatment with direct-acting antiviral agents for chronic hepatitis C. After treatment, a significant reduction in C-reactive protein levels was found, and there was a trend towards reduction in carotid intima-media thickness and a statistically significant resolution of atherosclerotic plaque. Further studies are needed to confirm these findings and their impact on long-term cardiovascular outcomes.
ARCHIVES OF MEDICAL RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Cynthia Lucero-Obusan, Gina Oda, Anoshiravan Mostaghimi, Patricia Schirmer, Mark Holodniy
Summary: Praedico is a customizable surveillance and data analytics platform built on big data technologies. The system has been evaluated and found to be simple, flexible, and stable, with acceptable performance in terms of representativeness, timeliness, sensitivity, and Predictive Value Positive. However, there are still areas for improvement, including data quality and user acceptability. The system has the potential to be used for interdepartmental and interagency collaboration and public health data sharing.
Article
Immunology
Aditya Sharma, Gina Oda, Mark Holodniy
Summary: The effectiveness of mRNA-based vaccines following the emergence of the SARS-CoV-2 Omicron variant was evaluated. The study found that BNT162b2 and mRNA-1273 were effective against COVID-19, and a third dose provided additional protection over the primary series.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
Eleftherios Mylonakis
Summary: This study aimed to investigate whether Ensovibep improves clinical outcomes for hospitalized COVID-19 patients. The results showed no significant differences in pulmonary outcomes and recovery rates between Ensovibep and placebo. The safety assessment did not identify any concerns.
ANNALS OF INTERNAL MEDICINE
(2022)
Article
Medicine, General & Internal
Yinong Young-Xu, Gabrielle M. Zwain, Hector S. Izurieta, Caroline Korves, Ethan Powell, Jeremy Smith, Abirami Balajee, Mark Holodniy, David O. Beenhouwer, Maria C. Rodriguez-Barradas, Sheldon T. Brown, Vincent C. Marconi
Summary: This study investigated the effectiveness of mRNA booster doses among vaccinated veterans and found that the booster was highly effective against infection, hospitalization, and death. Although the effectiveness against Delta variant infection was moderately higher than the Omicron variant, the effectiveness against severe disease and death was similarly high for both variants.
Article
Immunology
Neha Balachandran, Jordan Cates, Anita K. Kambhampati, Vincent C. Marconi, Alexis Whitmire, Elena Morales, Sheldon T. Brown, Diki Lama, Maria C. Rodriguez-Barradas, Rosalba Gomez Moronez, Gilberto Rivera Domiguez, David O. Beenhouwer, Aleksandra Poteshkina, Zlatko Anthony Matolek, Mark Holodniy, Cynthia Lucero-Obusan, Madhuri Agarwal, Cristina Cardemil, Umesh Parashar, Sara A. Mirza
Summary: This study identified risk factors for acute gastroenteritis (AGE) among hospitalized adults in the United States. Factors such as immunosuppressive therapy, HIV infection, severe renal disease, and household contact with an AGE patient were associated with an increased risk of AGE. Norovirus infection and severe AGE were also found to be correlated.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Gina Oda, Aditya Sharma, Cynthia Lucero-Obusan, Patricia Schirmer, Mark Holodniy
Summary: This study aims to determine the characteristics and sources of exposure in veterans with elevated blood lead levels. The results showed that firearms were the largest exposure source among veterans with elevated BLL. Potential conditions associated with lead exposure included attention-deficit/hyperactivity disorder and nausea or vomiting.
AMERICAN JOURNAL OF PUBLIC HEALTH
(2022)
Article
Medicine, General & Internal
Angela J. Rogers, Deborah Wentworth, Andrew Phillips, Katy Shaw-Saliba, Robin L. Dewar, Neil R. Aggarwal, Abdel G. Babiker, Weizhong Chang, Nila J. Dharan, Victoria J. Davey, Elizabeth S. Higgs, Norman Gerry, Adit A. Ginde, J. W. Awori Hayanga, Helene Highbarger, Jeroen L. Highbarger, Mamta K. Jain, Virginia Kan, Kami Kim, Perrine Lallemand, Bradley G. Leshnower, Joseph K. Lutaakome, Gail Matthews, Ahmad Mourad, Eleftherios Mylonakis, Ven Natarajan, Maria L. Padilla, Lavannya M. Pandit, Roger Paredes, Sarah Pett, Srikanth Ramachandruni, M. Tauseef Rehman, Brad T. Sherman, D. Clark Files, Samuel M. Brown, Michael A. Matthay, B. Taylor Thompson, James D. Neaton, H. Clifford Lane, Jens D. Lundgren
Summary: This study conducted a cross-sectional study of plasma antigen levels in 2540 hospitalized patients with COVID-19, finding that plasma antigen levels were closely associated with the severity of pulmonary disease and clinically important outcomes, with multiple clinical and viral factors associated with baseline plasma antigen levels.
ANNALS OF INTERNAL MEDICINE
(2022)
Article
Infectious Diseases
Tseli Mohammed, Jessica V. V. Brewer, Mary Pyatt, Stacey B. Whitbourne, J. Michael Gaziano, Connor Edson, Mark Holodniy
Summary: This study tested the feasibility of home blood sample collection methods to determine COVID-19 infection or vaccination status. Participants were randomly assigned to use two different devices and rated their experience. The Abbott assay showed the highest sensitivity in detecting known COVID infection and/or vaccination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Patricia Schirmer, Aditya Sharma, Cynthia Lucero-Obusan, Gina Oda, Mark Holodniy
Summary: The study evaluated the adherence to CDC guidelines for repeat chlamydia/gonorrhea testing and testing for HIV/syphilis in the US Veterans Health Administration. The results showed that nearly two-thirds of patients did not receive recommended repeat testing, and nearly one-third were not tested for HIV/syphilis. Additional education on CDC-recommended sexually transmitted infection guidelines and testing recommendations may be beneficial for healthcare providers in the Veterans Health Administration.
SEXUALLY TRANSMITTED DISEASES
(2023)
Article
Immunology
John B. Harley, Saiju Pyarajan, Elizabeth S. Partan, Lauren Epstein, Jason A. Wertheim, Abhinav Diwan, Christopher W. Woods, Victoria Davey, Sharlene Blair, Dennis H. Clark, Kenneth M. Kaufman, Shagufta Khan, Iouri Chepelev, Alexander Devine, Perry Cameron, Monica F. McCann, Mary Cloud B. Ammons, Devin D. Bolz, Jane K. Battles, Jeffrey L. Curtis, Mark Holodniy, Vincent C. Marconi, Charles D. Searles, David O. Beenhouwer, Sheldon T. Brown, Jonathan P. Moorman, Zhi Q. Yao, Maria C. Rodriguez-Barradas, Shyam Mohapatra, Osmara Y. Molina De Rodriguez, Emerson B. Padiernos, Eric R. McIndoo, Emily Price, Hailey M. Burgoyne, Ian Robey, Dawn C. Schwenke, Carey L. Shive, Ronald M. Przygodzki, Rachel B. Ramoni, Holly K. Krull, Robert A. Bonomo
Summary: The US Department of Veterans Affairs has established a biorepository to provide resources for the study of COVID-19 and future emerging diseases.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Genetics & Heredity
Margaret G. Guo, David L. Reynolds, Cheen E. Ang, Yingfei Liu, Yang Zhao, Laura K. H. Donohue, Zurab Siprashvili, Xue Yang, Yongjin Yoo, Smarajit Mondal, Audrey Hong, Jessica Kain, Lindsey Meservey, Tania Fabo, Ibtihal Elfaki, Laura N. Kellman, Nathan S. Abell, Yash Pershad, Vafa Bayat, Payam Etminani, Mark Holodniy, Daniel H. Geschwind, Stephen B. Montgomery, Laramie E. Duncan, Alexander E. Urban, Russ B. Altman, Marius Wernig, Paul A. Khavari
Summary: Noncoding variants play a role in the heritability of neuropsychiatric diseases. A study in developing human neural cells investigated 2,221 noncoding variants associated with ten neuropsychiatric disorders and identified differentially-active single-nucleotide variants (daSNVs) in specific neural cell types. Integrating epigenomic and transcriptomic data helped identify candidate disease-relevant target genes modulated by these daSNVs.
Article
Immunology
Jennifer M. Ross, Jonathan D. Sugimoto, Andrew Timmons, Jonathan Adams, Katrina Deardoff, Anna Korpak, Cindy Liu, Kathryn Moore, Deanna Wilson, Roger Bedimo, Kyong-Mi Chang, Kelly Cho, Kristina Crothers, Eric Garshick, J. Michael Gaziano, Mark Holodniy, Christine M. Hunt, Stuart N. Isaacs, Elizabeth Le, Barbara E. Jones, Javeed A. Shah, Nicholas L. Smith, Jennifer S. Lee
Summary: In a cohort of hospitalized US veterans with SARS-CoV-2 infection, those with a higher Charlson comorbidity index had more severe COVID-19 illness, longer hospital stays, and higher rates of rehospitalization.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Cynthia Lucero-Obusan, Rishi Deka, Patricia Schirmer, Gina Oda, Mark Holodniy
Summary: This study describes the epidemiology of coccidioidomycosis among a national cohort of Veterans and identifies certain demographic factors associated with the infection. The highest incidence rates and case counts were observed in specific counties in Arizona and California. The study highlights the increased risk of severe coccidioidomycosis among specific racial and ethnic groups.
Article
Immunology
Tomas O. Jensen, Greg A. Grandits, Mamta K. Jain, Thomas A. Murray, Birgit Grund, Kathryn Shaw-Saliba, Michael A. Matthay, Mahsa Abassi, Magdalena Ardelt, Jason V. Baker, Peter Chen, Robin L. Dewar, Anna L. Goodman, Timothy J. Hatlen, Helene C. Highbarger, Mark Holodniy, Perrine Lallemand, Sylvain Laverdure, Bradley G. Leshnower, David Looney, Charalampos D. Moschopoulos, Henry Mugerwa, Daniel D. Murray, Eleftherios Mylonakis, Stephanie Nagy-Agren, M. Tauseef Rehman, Adam Rupert, Randy A. Stevens, Stuart Turville, Amy Weintrob, Katherine Wick, Jens Lundgren, Emily R. Ko
Summary: This study suggests that neutralizing monoclonal antibodies have an antiviral effect among hospitalized patients with COVID-19, without affecting the endogenous anti-nucleocapsid antibody response, systemic inflammation, or clinical status on day 5.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Jordan Cates, Cristina Cardemil, Sara A. Mirza, Ben Lopman, Aron J. Hall, Mark Holodniy, Cynthia Lucero-Obusan
Summary: Norovirus infection was associated with a slight increased risk of hospitalization and significant increased risk of mortality within 3 days of norovirus diagnosis among a large national cohort of US veterans.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)