Article
Infectious Diseases
Maria V. Golikova, Elena N. Strukova, Kamilla N. Alieva, Vladimir A. Ageevets, Alisa A. Avdeeva, Ofeliia S. Sulian, Stephen H. Zinner
Summary: The minimal inhibitory concentration (MIC) is used to determine bacterial susceptibility or resistance to antibiotics and predict clinical efficacy. Other measures of resistance include the MIC determined at high bacterial inocula (MICHI) and the mutant prevention concentration (MPC). This study analyzed the resistance profiles of different K. pneumoniae strains based on meropenem susceptibility, carbapenemase production, and specific carbapenemase types. The analysis showed that MIC and MPC did not correlate, but there was a significant correlation between MICHI and MPC, indicating similar resistance properties. Determining MICHI can help assess the resistance-related risk of a specific K. pneumoniae strain.
Article
Microbiology
Yanyan Zhang, Danxia Gu, Xuemei Yang, Yuchen Wu, Congcong Liu, Zhangqi Shen, Rong Zhang
Summary: In this study, a carbapenemase-producing Klebsiella michiganensis strain was identified from a patient with Guillain-Barre syndrome. The strain exhibited resistance to multiple antibiotics and carried multiple resistance genes. The findings suggest that this strain may facilitate the dissemination of resistance genes through horizontal transfer and nosocomial spread.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Pinar Zarakolu, Ozgen Koseoglu Eser, Baris Otlu, Oznur Gurpinar, Cuneyt Ozakin, Halis Akalin, Iftihar Koksal, Serhat Unal
Summary: This study aimed to assess the activity of fosfomycin against E. coli and K. pneumoniae isolates and the frequency of carbapenemase types in CR groups. Fosfomycin demonstrated significant in-vitro activity against CS and CR E. coli, and CS K. pneumoniae, with emerging resistance in CR K. pneumoniae and OXA-48 being the predominant carbapenemase type in Turkey.
JOURNAL OF CHEMOTHERAPY
(2022)
Article
Microbiology
Xinmiao Jia, Peiyao Jia, Ying Zhu, Wei Yu, Xue Li, Jingyuan Xi, Xiaoyu Liu, Kang Liao, Yingchun Xu, Bin Cheng, Qiwen Yang
Summary: We identified a novel hybrid plasmid carrying both bla(NDM-1) and bla(IMP-4) genes, and characterized its genetic and phenotypic features. The plasmid showed potential for horizontal transfer and high stability, without exerting significant growth pressure on bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Serpil Genc, Fetiye Kolayli, Eda Yazici Ozcelik
Summary: This study demonstrates the increasing incidence of bla(NDM) reaching endemic levels, in addition to the endemic bla(OXA-48) in Turkey. It is noteworthy that this is the first report of the co-production of bla(KPC) + NDM and bla(OXA-48 + KPC) in CRKP isolates.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Article
Chemistry, Medicinal
Clyde A. Smith, Nichole K. Stewart, Marta Toth, Pojun Quan, John D. Buynak, Sergei B. Vakulenko
Summary: The widespread presence of carbapenem-hydrolyzing beta-lactamases in Gram-negative bacteria has reduced the effectiveness of carbapenem antibiotics, limiting treatment options. In the Enterobacteriaceae family, production of class D beta-lactamases, specifically from the OXA-48-type family, is a major mechanism of resistance. This study evaluates a novel carbapenem, NA-1-157, which shows promise in treating infections caused by OXA-48-producing bacterial pathogens.
ACS INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Nouf Al-Rashed, Khalid M. Bindayna, Mohammad Shahid, Nermin Kamal Saeed, Abdullah Darwish, Ronni Mol Joji, Ali Al-Mahmeed
Summary: This study collected 50 carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from four main hospitals in Bahrain. The results showed that all isolates were resistant to imipenem, meropenem, and cephalosporins, and harbored various resistance genes. The study emphasizes the importance of strengthening surveillance and stringent infection control measures in clinical settings to prevent the further spread of such isolates.
Article
Infectious Diseases
Souad Loqman, Nabila Soraa, Seydina M. Diene, Jean-Marc Rolain
Summary: The study investigated the occurrence of carbapenem-resistant Enterobacteriaceae (CRE) among hospitalized patients in Mohamed VI University Hospital in Marrakech, Morocco. It found various bacterial strains resistant to carbapenems, with Klebsiella pneumoniae being the most common species. The study also identified several carbapenemase-producing isolates, with blaOXA-48 genes being documented for the first time in Citrobacter freundii and Citrobacter braakii in Morocco.
Article
Chemistry, Medicinal
Eugenie Romero, Saoussen Oueslati, Mohamed Benchekroun, Agathe C. A. D. Hollander, Sandrine Ventre, Kamsana Vijayakumar, Corinne Minard, Cynthia Exilie, Linda Tlili, Pascal Retailleau, Agustin Zavala, Eddy Elisee, Edithe Selwa, Laetitia A. Nguyen, Alain Pruvost, Thierry Naas, Bogdan I. Iorga, Robert H. Dodd, Kevin Cariou
Summary: A series of imino-analogues of beta-lactams have been developed as efficient non-covalent inhibitors of beta-lactamases, showing inhibitory effects on three major carbapenemases and a cephalosporinase.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Laura Dabos, Joanna E. Raczynska, Pierre Bogaerts, Agustin Zavala, Delphine Girlich, Remy A. Bonnin, Laurent Dortet, Aurelie Peyrat, Pascal Retailleau, Bogdan I. Iorga, Mariusz Jaskolski, Youri Glupczynski, Thierry Naas
Summary: OXA-48-producing Enterobacterales have disseminated globally, with several variants reported, differing by a few amino acid substitutions or deletions, mostly in the /35-/36 loop region. OXA-517, a natural variant of OXA-48, with an Arg214Lys substitution and a deletion in the β5-β6 loop, has the ability to hydrolyze extended-spectrum cephalosporins and carbapenems. This study highlights the remarkable ability of OXA-48-like carbapenemases to evolve through mutation/deletion in the β5-β6 loop to expand their hydrolysis spectrum.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Junsung Hong, Dayoung Kang, Dokyun Kim
Summary: The study evaluated the performance of the RESIST-5 O.K.N.V.I. assay for identifying common carbapenemases in clinical isolates, showing high sensitivity (98.4%) and specificity (100%). The assay accurately identified non-targeted carbapenemase producers and non-CP CREs as negative results.
Article
Chemistry, Medicinal
Doris Mia Taylor, Justin Anglin, Liya Hu, Lingfei Wang, Banumathi Sankaran, Jin Wang, Martin M. Matzuk, B. V. Venkataram Prasad, Timothy Palzkill
Summary: This study screened various low-molecular-weight compounds against OXA-48 and identified functional scaffolds for inhibitor development with key interactions revealed. Some low-micromolar-potency inhibitors synergize with ampicillin to kill Escherichia coli expressing OXA-48, albeit at high concentrations of the respective inhibitors. Additionally, several compounds showed micromolar potency toward OXA-24 and OXA-58 class D carbapenemases prevalent in Acinetobacter baumannii.
ACS INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Remy A. Bonnin, Cecile Emeraud, Agnes B. Jousset, Thierry Naas, Laurent Dortet
Summary: The study compared different methods for cefiderocol susceptibility testing and found that the CE-IVD BMD plate showed high consistency, while the MIC test strip's underestimation could lead to errors. Disk diffusion may be useful for screening, but all CRE should be re-tested using BMD for definitive categorization.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Stacey Ceron, Zackary Salem-Bango, Deisy A. Contreras, Elizabeth L. Ranson, Shangxin Yang
Summary: This study aimed to investigate the clinical and genomic characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) co-producing New Delhi metallo-β-lactamases (NDM) and oxacillinase 48 (OXA-48)-like carbapenemases isolated from patients in Southern California since 2016. The results showed genetic diversity in the CRKP isolates, with various lineages and antimicrobial resistance genes identified. Patients infected with this rare type of CRKP had limited treatment options and poor clinical outcomes, highlighting the importance of real-time genomic surveillance for infection prevention and treatment guidance.
Article
Infectious Diseases
Fatma Ben Abid, Clement K. M. Tsui, Yohei Doi, Anand Deshmukh, Christi L. McElheny, William C. Bachman, Erin L. Fowler, Ahmed Albishawi, Kamran Mushtaq, Emad B. Ibrahim, Sanjay H. Doiphode, Manal M. Hamed, Muna A. Almaslmani, Abdullatif Alkhal, Adeel A. Butt, Ali S. Omrani
Summary: The study conducted whole genome sequencing and multi-locus sequence typing on 149 carbapenem-resistant Enterobacterales obtained from clinical samples between April 2014 and November 2017. The most common species were Klebsiella pneumoniae and Escherichia coli, with genes encoding metallo-beta-lactamases and OXA-48-like enzymes being frequently detected in the isolates.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Samanta Freire, Teresa Grilo, Patrice Nordmann, Laurent Poirel, Marta Aires-de-Sousa
Summary: A rapid and reliable multiplex PCR was developed to simultaneously detect known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing fosA, fosC, and fosL-like genes, demonstrating a sensitivity and specificity of 100%.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Mustafa Sadek, Remi Le Guern, Eric Kipnis, Philippe Gosset, Laurent Poirel, Rodrigue Dessein, Patrice Nordmann
Summary: This study reports the development of cefiderocol resistance among sequential Pseudomonas aeruginosa clinical isolates, without exposure to the drug. The resistance was driven by multifactorial mechanisms, including mutational changes in iron transporter proteins associated with drug uptake and overexpression of certain genes.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Maxime Bouvier, Valeria Gaia, Patrice Nordmann
Summary: Carbapenem-resistant Enterobacterales, including KPC-producing Klebsiella pneumoniae, pose a major threat to public health due to their rapid spread. Ceftazidime-avibactam, a beta-lactam/beta-lactamase inhibitor combination, has shown excellent activity against multidrug-resistant KPC-producing Enterobacterales strains. However, there is an increasing occurrence of CAZ-AVI-resistant K. pneumoniae isolates, mainly due to the emergence of KPC variants that confer resistance to CAZ-AVI but retain carbapenem resistance.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Mustafa Sadek, Laurent Poirel
Summary: This study evaluated the in vitro activity of cefepime/taniborbactam and cefepime/zidebactam against aztreonam/avibactam-resistant E. coli strains. The results showed that cefepime/taniborbactam had a resistance rate of 100% against these strains, while cefepime/zidebactam was effective in all cases. This suggests that cefepime/zidebactam combination could be a promising therapeutic option.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Ahmad Ibrahim, Maxime Bouvier, Mustafa Sadek, Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Summary: Cefiderocol (FDC) is an effective siderophore cephalosporin against multidrug-resistant Gram-negative bacteria. The SuperFDC medium was developed to rapidly and accurately identify FDC-resistant pathogens, which is crucial for controlling their spread. The medium showed high sensitivity and specificity in detecting FDC-resistant isolates with various resistance mechanisms.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
Summary: A study was conducted to select MEM-VAB resistant mutants and decipher the resistance mechanisms. The mutants showed reduced susceptibility to various antibiotics and had mutations in the ompK36 gene. Complementation with the wildtype ompK36 gene reduced the minimal inhibitory concentrations. Additionally, the bla KPC gene copy numbers were significantly increased in the resistant mutants.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Chloe Buchs, Doris Yoong Wen Di, Gian Maria Rossolini, Roger Stephan, Mariana Castanheira, Laurent Poirel
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Otavio Hallal Ferreira Raro, Laurent Poirel, Maurine Tocco, Patrice Nordmann
Summary: The study aimed to evaluate whether subdosages of antibiotics administered in veterinary medicine could enhance plasmid transfer and resistance gene exchange in gut microbiota. The results showed that antibiotics in veterinary medicine may induce the transfer of plasmid-encoded resistance and contribute to the global spread of antibiotic resistance genes.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Editorial Material
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Clement Viguier, Maxime Bouvier, Mustafa Sadek, Auriane Kerbol, Laurent Poirel, Patrice Nordmann
Summary: This study develops a rapid culture-based test for the identification of Aztreonam-avibactam resistance among multidrug-resistant Enterobacterales. The test is rapid, highly sensitive, specific, easily interpretable, and can be easily implemented in routine laboratory practices.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Teresa Grilo, Samanta Freire, Bruno Miguel, Luis Nogueira Martins, Maria Favila Menezes, Patrice Nordmann, Laurent Poirel, Maria Jose Rego Sousa, Marta Aires-de-Sousa
Summary: This study evaluated the occurrence of plasmid-mediated fos genes among fosfomycin-resistant Escherichia coli isolates collected from patients in Lisbon, Portugal. The results showed a low prevalence of fosfomycin-resistant E. coli isolates, but 15% of these isolates carried transferable fosA genes, and a high rate of extended-spectrum beta-lactamase (ESBL) production was observed.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
