Article
Infectious Diseases
Dusten T. Rose, Alexander Moskhos, Arya Wibisono, Kelly R. Reveles
Summary: This study evaluated the impact of using MicroScan and VITEK 2 automated systems to determine vancomycin MIC values on the treatment of MRSA bacteremia. The results showed that the MicroScan group had a higher rate of vancomycin alternative therapy use compared to the VITEK 2 group, while the VITEK 2 group had a shorter median hospital stay.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Warren Rose, Michael Fantl, Matthew Geriak, Victor Nizet, George Sakoulas
Summary: Literature has shown the effectiveness of combination antimicrobial therapy in treating refractory MRSA bacteremia, but considerations need to be made on which regimens are more beneficial, potential harms, and which patients can benefit from more aggressive treatment. In addition, combination therapy for MRSA bacteremia does not need to be uniform throughout the treatment course, with options for early intensive treatment and later consolidation therapy.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
George Sakoulas
Summary: Given the difficulty of obtaining answers from randomized clinical trials for treating challenging bacteremic infections caused by methicillin-resistant Staphylococcus aureus, clinicians, microbiologists, and pharmacists need to collaborate to find new individualized antimicrobial therapies. One successful example involved using imipenem/cilastatin plus fosfomycin to treat a particularly stubborn MRSA bacteremia and spinal abscess.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Erin E. Zwack, Ze Chen, Joseph C. Devlin, Zhi Li, Xuhui Zheng, Ada Weinstock, Keenan A. Lacey, Edward A. Fisher, David Fenyo, Kelly V. Ruggles, P'ng Loke, Victor J. Torres
Summary: Infection with Staphylococcus aureus weakens the transcriptional response and downregulates genes related to innate immune response and cytokine signaling, leading to impaired neutrophil recruitment. This transcriptional suppression is conserved across different S. aureus clones and is absent in blood exposed to heat-killed S. aureus or infected with less virulent Staphylococcus epidermidis. The master regulator S. aureus exoprotein expression and its regulated pore-forming toxins are key mediators of this transcriptional suppression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Rozeen Abu Shqara, Imad Kassis, Ranaa Damouni Shalabi, Halima Dabaja Younis
Summary: This study aimed to investigate the clinical features, antibiotic susceptibility, mortality rate, and risk factors of mortality in children with Pseudomonas bacteremia. The results showed relatively lower mortality rate in children with Pseudomonas bacteremia, and younger age, multidrug-resistant isolates, and inappropriate antibiotic treatment were associated with higher mortality.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Immunology
Miquel Pujol, Jose-Maria Miro, Evelyn Shaw, Jose-Maria Aguado, Rafael San-Juan, Mireia Puig-Asensio, Carles Pigrau, Esther Calbo, Miguel Montejo, Regino Rodriguez-Alvarez, Maria-Jose Garcia-Pais, Vicente Pintado, Rosa Escudero-Sanchez, Joaquin Lopez-Contreras, Laura Morata, Milagros Montero, Marta Andres, Juan Pasquau, Maria-del-Mar Arenas, Belen Padilla, Javier Murillas, Alfredo Jover-Saenz, Luis-Eduardo Lopez-Cortes, Graciano Garcia-Pardo, Oriol Gasch, Sebastian Videla, Pilar Hereu, Cristian Tebe, Natalia Pallares, Mireia Sanllorente, Maria-Angeles Dominguez, Jordi Camara, Anna Ferrer, Ariadna Padulles, Guillermo Cuervo, Jordi Carratala
Summary: The study found that daptomycin plus fosfomycin provided a slightly higher treatment success rate for MRSA bacteremia and endocarditis compared to daptomycin alone, but this difference was not statistically significant. The antibiotic combination was associated with lower rates of microbiological failure and complicated bacteremia, but was also more frequently linked to adverse events.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Biology
Akanksha Rajput, Saugat Poudel, Hannah Tsunemoto, Michael Meehan, Richard Szubin, Connor A. Olson, Yara Seif, Anne Lamsa, Nicholas Dillon, Alison Vrbanac, Joseph Sugie, Samira Dahesh, Jonathan M. Monk, Pieter C. Dorrestein, Rob Knight, Joe Pogliano, Victor Nizet, Adamm. Feist, Bernhard O. Palsson
Summary: The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.
Article
Infectious Diseases
Helio S. Sader, Cecilia G. Carvalhaes, Rodrigo E. Mendes
Summary: The study evaluated the antimicrobial activity of Ceftaroline against Staphylococcus aureus isolated from patients with infective endocarditis. Ceftaroline demonstrated potent in vitro activity against a large collection of S. aureus isolates recovered from patients with bloodstream infections, including IE. Ceftaroline susceptibility rates were higher in North America and LATAM-APAC compared to Europe.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Organic
Jian Li, Hans Renata
Summary: A novel chemoenzymatic approach was developed for the synthesis of fasamycin A, a halogenated naphthacenoid with activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis. The synthesis involved combining two fragments through a Sammes annulation to form a dimethylnaphthacenone system, followed by enzymatic halogenation to introduce the necessary chlorine substituent at a later stage.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Chemistry, Medicinal
Santosh Kumar Verma, Rameshwari Verma, Kothanahally S. Sharath Kumar, Laxmi Banjare, Afzal B. Shaik, Richie R. Bhandare, Kadalipura P. Rakesh, Kanchugarakoppal S. Rangappa
Summary: MRSA is becoming a major concern due to its rapid resistance development, prompting the need for novel antibiotics. The oxadiazole moiety serves as an interesting and adaptable molecular structure with potential for research and development as antibacterial agents. Studies on oxadiazole-containing derivatives as potent antibacterial agents against multidrug-resistant MRSA strains and exploring structure-activity relationships have been conducted, providing valuable insights for future drug development.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Chemistry, Medicinal
Rameshwari Verma, Santosh Kumar Verma, Kadalipura P. Rakesh, Yarabahally R. Girish, Milad Ashrafizadeh, Kothanahally S. Sharath Kumar, Kanchugarakoppal S. Rangappa
Summary: MRSA poses a deadly threat to humanity due to its easy transmission and resistance to antibiotics, including vancomycin. The development of new, efficient, and nontoxic drug candidate to fight against MRSA isolates is urgently needed. Research on pyrazole-containing derivatives has shown promise in combating MRSA with a broad antibacterial movement.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
David B. Huang, Eric Gaukel, Nancy Kerzee, Katyna Borroto-Esoda, Simon Lowry, Yan Q. Xiong, Wessam Abdelhady, Arnold S. Bayer
Summary: The combined efficacy of lysin LSVT-1701 plus daptomycin was highly effective at reducing MRSA counts in target tissue, suggesting its potential as an adjunctive therapy for invasive MRSA infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Joe Amoah, Eili Y. Klein, Kathleen Chiotos, Sara E. Cosgrove, Pranita D. Tamma
Summary: According to a multicenter observational study, prioritizing administration of beta-lactam over vancomycin for critically ill patients with suspected bloodstream infections may reduce early mortality, highlighting the significant impact of a simple practice change on improving patient survival.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Shu-Chen Kuo, Mei-Chen Tan, Wei-Cheng Huang, Han-Chieh Wu, Feng-Jui Chen, Yu-Chieh Liao, Hui-Ying Wang, Yih-Ru Shiau, Tsai-Ling Lauderdale
Summary: The study aimed to determine the susceptibilities of Elizabethkingia spp. to various antibiotics, revealing differences in susceptibility among different strains. Discrepancies were observed between the results obtained from traditional methods and an automated system.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Sang Hyun Ra, Ji-Soo Kwon, Ji Yeun Kim, Hye-Hee Cha, Hyun-Jung Lee, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Won Young Kim, Sung-Han Kim
Summary: The study evaluated the presence of salivary VZV DNA in patients with acute abdominal pain and its potential relation to enteric zoster. Approximately 10% of patients showed positivity for salivary VZV DNA. Further research is needed to investigate whether antiviral therapy based on VZV DNA results may relieve abdominal pain.
INFECTIOUS DISEASES
(2021)
Letter
Critical Care Medicine
Hyemin Chung, Sang-Bum Hong, Jin Won Huh, Heungsup Sung, Kyung-Hyun Do, Sang-Oh Lee, Chae-Man Lim, Younsuck Koh, Sang-Ho Choi
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Infectious Diseases
Yun Woo Lee, So Yun Lim, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Yang Soo Kim, Sang-Ho Choi
Summary: This study evaluated the clinical characteristics and outcomes of Enterococcus raffinosus bacteremia in adults. The study found that E. raffinosus bacteremia mainly originated from biliary tract infection, had high resistance rates to penicillin and ampicillin, but had a low bacteremia-related mortality rate.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Haein Kim, Hyeonji Seo, Hyemin Chung, Sunghee Park, Heungsup Sung, Mi-Na Kim, Seongman Bae, Jiwon Jung, Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Yong Pil Chong
Summary: The value of follow-up blood culture (FUBC) in the management of Gram-negative bacteremia (GNB) is controversial. This study evaluated bedside risk predictors and found that hemodialysis, fever on the day of FUBCs, intravascular device, no use of in vitro active antibiotic within 24 h, non-fermenting bacteria, and multidrug resistance were independent risk factors for positive FUBCs in GNB. The yield of FUBCs increased as the number of bedside risk predictors increased, and positive FUBCs were significantly associated with 30-day mortality.
Article
Infectious Diseases
Moonsuk Bae, Yunseo Jeong, Seongman Bae, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jiwon Jung
Summary: The study found that in patients with uncomplicated Pseudomonas aeruginosa bloodstream infection, there was no significant difference in recurrence rates or 30-day mortality between short-course and prolonged-course antimicrobial therapy. Prolonged therapy did not significantly reduce the risk of P. aeruginosa infection recurrence within 180 days compared to short-course therapy.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Kyung Hwa Jung, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Sung-Han Kim
Summary: The study identified hematologic malignancy and ulcerative colitis as independent risk factors for the relapse of gastrointestinal cytomegalovirus disease. Approximately 10% of the participants experienced disease relapse after antiviral treatment. Prolonged antiviral treatment may not be helpful in preventing relapse of GI CMV disease.
Article
Virology
Hyemin Chung, Eunsil Kim, Bomin Kwon, Yeong-Geon Cho, Seongman Bae, Jiwon Jung, Min-Jae Kim, Yong-Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang-Soo Kim
Summary: This study investigated the kinetics of Gn-specific antibodies in patients with severe fever with thrombocytopenia syndrome (SFTS). The results showed that the peak of anti-Gn-specific IgM occurred later than that of anti-NP-specific IgM, and anti-Gn-specific IgG lasted for at least 3 years.
Article
Immunology
Hyeonji Seo, Jeong-Young Lee, Seung Hee Ryu, Sun Hee Kwak, Eun Ok Kim, Seongman Bae, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Heungsup Sung, Mi-Na Kim, Yang Soo Kim, Jiwon Jung
Summary: The study suggests that patients with negative CPE culture had lower rates of infection and transmission compared to those with positive CPE culture. The Xpert Carba-R assay is clinically useful not only for rapid identification of CPE but also for predicting the risks of infection and transmission when performed along with culture.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Critical Care Medicine
Jin-Young Huh, Sang-Ho Choi, Kyung-Wook Jo, Jin Won Huh, Sang-Bum Hong, Tae Sun Shim, Chae-Man Lim, Younsuck Koh
Summary: This study investigated the risk factors for progression from non-severe Legionella pneumonia (LP) to severe LP. The results showed that a low platelet count and delayed antibiotic treatment were significantly associated with the progression to severe LP from non-severe LP.
ACUTE AND CRITICAL CARE
(2022)
Article
Medicine, General & Internal
Jiyoung Lee, Euijin Chang, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang -Oh Lee, Sang -Ho Choi, Yang Soo Kim, Seongman Bae
Summary: This study evaluated the rate and composition of bacterial co-infection in COVID-19 patients and assessed the association between the use of empirical antibacterials and mortality. The results showed that bacterial co-infection was infrequent in the patients, and the co-infected organisms varied depending on the previous use of antibacterial drugs.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
Review
Infectious Diseases
Sang -Ho Choi, Antoni Cesar, Timothy Arthur Chandos Snow, Naveed Saleem, Nishkantha Arulkumaran, Mervyn Singer
Summary: For hospitalized adults with mild-to-moderate community-acquired pneumonia (CAP), respiratory fluoroquinolone monotherapy or β-lactam plus macrolide combination therapy are suggested as first-line options according to guidelines. However, the efficacy of these regimens has not been adequately evaluated.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Yun Woo Lee, Jin Won Huh, Sang-Bum Hong, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Heungsup Sung, Kyung-Hyun Do, Sang-Oh Lee, Chae-Man Lim, Yang Soo Kim, Younsuck Koh, Sang-Ho Choi
Summary: We investigated the distribution and characteristics of severe Corynebacterium striatum pneumonia in South Korea from 2014 to 2019. The majority of cases were hospital-acquired and more than half of the patients were immunocompromised. The proportion of C. striatum cases among patients with severe hospital-acquired pneumonia increased over time, while methicillin-resistant Staphylococcus aureus (MRSA) infections decreased. C. striatum was responsible for a significant proportion of severe hospital-acquired pneumonia cases, and the mortality rates were high, similar to those caused by MRSA. This pathogen is emerging as a possible cause of severe pneumonia, especially among immunocompromised patients.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Hyeonji Seo, Haein Kim, Seongman Bae, Seonghee Park, Hyemin Chung, Heung-Sup Sung, Jiwon Jung, Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Ki Young Son, Yong Pil Chong
Summary: This study found no significant differences in clinical deterioration between patients treated with fluvoxamine and placebo among adult patients with symptomatic COVID-19 admitted to the CTC. Factors such as age, fever, and pneumonia may be independent risk factors for clinical deterioration.
INFECTION AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Tark Kim, Seong Yeon Park, Yee Gyung Kwak, Jiwon Jung, Min-Chul Kim, Seong-Ho Choi, Shi Nae Yu, Hyo-Lim Hong, Yong Kyun Kim, Se Yoon Park, Eun Hee Song, Ki-Ho Park, Oh Hyun Cho, Sang-Ho Choi
Summary: In Korea, Staphylococcus aureus and streptococci are the main pathogens causing community-onset Pyomyositis, with MRSA being less common in these cases. Enteric Gram-negative organisms should be considered major etiologies, especially in the healthcare-associated Pyomyositis patient population in Korea.
INFECTION AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
