Article
Immunology
Bongyoung Kim, Jeoungyeon Kim, Hyun-uk Jo, Ki Tae Kwon, Seong-yeol Ryu, Seong-Heon Wie, Jieun Kim, Se Yoon Park, Kyung-Wook Hong, Hye In Kim, Mi Hyun Bae, Hyun ah Kim, Mi-Hee Kim, Yong-Hak Sohn, Yangsoon Lee, Hyunjoo Pai
Summary: This study examines the changes in characteristics of community-onset fluoroquinolone-resistant E. coli isolates causing community-acquired acute pyelonephritis in South Korea. The results show an increase in antimicrobial resistance against cephalosporins, as well as the prevalence of ST131 and the presence of ESBL and/or PMQR determinants in the isolates from 2017-2018 compared to those from 2010-2011.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Microbiology
Isidro Garcia-Menino, Pilar Lumbreras, Luz Leston, Monica Alvarez-Alvarez, Vanesa Garcia, Jens Andre Hammerl, Javier Fernandez, Azucena Mora
Summary: Analyzing the clonal structure and antimicrobial resistance of E. coli isolates in urinary tract infections is important for detecting changes in emerging uropathogenic clones and guiding optimal control and antibiotic prescribing.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Kwangwon Seo, Kyung-Hyo Do, Wan-Kyu Lee
Summary: This study investigated the molecular characteristics of fluoroquinolone-resistant E. coli isolates from suckling piglets with colibacillosis. The results showed that all isolates exhibited multi-drug resistance and mutations in quinolone resistance determining regions. Plasmid-mediated quinolone resistance genes were also identified, along with other resistance genes and toxin genes.
Article
Veterinary Sciences
Jin-Hyun Kim, Hye-Jin Lee, Ok-Mi Jeong, Dong-Wan Kim, Ji-Yeon Jeong, Yong-Kuk Kwon, Min-Su Kang
Summary: The study investigated the characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from chickens in Korea, revealing high resistance to multiple antibiotics and diverse genetic and fitness traits among the isolates. The findings suggest that fluoroquinolone-resistant APEC in poultry poses a potential risk to public health.
Article
Infectious Diseases
Soha S. Abdelrahim, Magdy Fouad, Nilly Abdallah, Rasha F. Ahmed, Shaimaa Zaki
Summary: The study identified a high prevalence of the O25-ST131 clone among E. coli isolates, with significantly higher rates of ESBL, MDR, and antibiotic resistance. The presence of the CTX-M-15 gene was notably high in intestinal isolates, while the O16-ST131 clone was not detected in the study samples.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Razib Mazumder, Arif Hussain, Jody E. E. Phelan, Susana Campino, S. M. Arefeen Haider, Araf Mahmud, Dilruba Ahmed, Md Asadulghani, Taane G. G. Clark, Dinesh Mondal
Summary: The study reveals the presence of multi-resistant non-lactose fermenting E. coli strains responsible for intestinal and extraintestinal infections. Whole-genome sequencing and analysis identified high-risk clonal lineages and important genetic features. The findings suggest that non-lactose fermenting E. coli strains can cause infections and highlight the need for monitoring and prevention strategies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Ayorinde O. Afolayan, A. Oladipo Aboderin, Anderson O. Oaikhena, Erkison Ewomazino Odih, Veronica O. Ogunleye, Adeyemi T. Adeyemo, Abolaji T. Adeyemo, Oyeniyi S. Bejide, Anthony Underwood, Silvia Argimon, Monica Abrudan, Abiodun Egwuenu, Chikwe Ihekweazu, David M. Aanensen, Iruka N. Okeke
Summary: In this study, the identity of circulating clones of Escherichia coli associated with bloodstream infections in South-Western Nigeria was investigated. High resistance to commonly used antimicrobials was observed, but susceptibility to carbapenems and colistin was found. The study also identified two clusters of isolates and a possible outbreak cluster of Escherichia coli.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Elena Salamanca-Rivera, Lorena Lopez-Cerero, Jose Manuel Rodriguez-Martinez, Alvaro Pascual, Jesus Rodriguez-Bano
Summary: Escherichia coli ST131 clade C is an important driver for fluoroquinolone resistance (FQ-R). This study found that long-term care facilities are important reservoirs of clade C ST131 E. coli, and cross-transmission is the most relevant transmission mechanism.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Nubwa Medugu, Isabella A. Tickler, Carissa Duru, Ruth Egah, Abu Ocheiku James, Vivian Odili, Fatima Hanga, Eyinade Kudirat Olateju, Binta Jibir, Bernard E. Ebruke, Grace Olanipekun, Fred C. Tenover, Stephen K. Obaro
Summary: This study characterized the phenotypic and genotypic profiles of multi-drug resistant Enterobacterales isolated from patients in Nigerian hospitals. The isolates showed high resistance rates to third-generation cephalosporins and carbapenems. The most common resistance genes were bla(TEM-1B), bla(CTX-M-15), and bla(NDM-1). High-risk clones and potential treatment options were also identified.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Jan Koren, Michal Andrezal, Elham Ozaee, Hana Drahovska, Martin Wawruch, Adriana Liptakova, Tibor Maliar
Summary: The study analyzed E. coli strains from two healthcare facilities in University Hospital Bratislava and found the emergence of ST131 ESBL-producing E. coli, which poses major challenges worldwide, with the majority originating from the first hospital. Additionally, investigation on ST131 and clonal relatedness indicated a possible epidemiological association.
Article
Infectious Diseases
Virginia Nunez-Samudio, Maydelin Pecchio, Gumercindo Pimentel-Peralta, Yohana Quintero, Mellissa Herrera, Ivan Landires
Summary: The study conducted in two hospitals in central Panama identified ESBL-producing E. coli strains as a significant public health concern in Central America, with the pandemic clone ST43/ST131 carrying CTX-M-15. This is the first report confirming the presence of this antimicrobial-resistant organism in inpatients and outpatients in the region, highlighting potential challenges in treating infections.
Article
Immunology
James R. Johnson, Connie Clabots, Stephen B. Porter, Tricia Bender, Brian D. Johnston, Paul Thuras
Summary: This study found that ST131-H30 subclone of Escherichia coli exhibits exceptional intestinal persistence, possibly due to a combination of fluoroquinolone resistance and virulence factors. These findings provide new insights for further study on the pandemic emergence of ST131-H30.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Public, Environmental & Occupational Health
Romain Martischang, Patrice Francois, Abdessalam Cherkaoui, Nadia Gaia, Gesuele Renzi, Americo Agostinho, Monica Perez, Christophe E. Graf, Stephan Harbarth
Summary: The study found that the prevalence of ESBL-EC belonging to the ST131 H30 subclone showed an increasing trend from 2010 to 2015, followed by a decline. Multiple unnoticed ESBL-EC outbreaks occurred in LTCFs, and clonal expansion of a rare ST131 H89 subclone was observed. Despite discontinuation of contact precautions for ESBL-EC carriers since 2019, no rebound effect was observed in ESBL-EC prevalence or different subclones.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Infectious Diseases
Kazuma Sakaeda, Takuya Sadahira, Yuki Maruyama, Takehiro Iwata, Masami Watanabe, Koichiro Wada, Motoo Araki
Summary: We conducted a molecular biological analysis of ESBL-producing E. coli strains and their sensitivity to FMOX. Most of the strains belonged to the ST-131 sequence type and had CTX-M genotypes. The isolated strains were sensitive to FMOX in drug susceptibility tests. FMOX showed efficacy for perioperative prophylactic administration in urologic surgery and for therapeutic administration for urinary tract infections.
Article
Critical Care Medicine
Sunita Mulpuru, Li Li, Lingyun Ye, Todd Hatchette, Melissa K. Andrew, Ardith Ambrose, Guy Boivin, William Bowie, Ayman Chit, Gael Dos Santos, May Elsherif, Karen Green, Francois Haguinet, Scott A. Halperin, Barbara Ibarguchi, Jennie Johnstone, Kevin Katz, Joanne M. Langley, Jason LeBlanc, Mark Loeb, Donna Mackinnon-Cameron, Anne McCarthy, Janet E. McElhaney, Allison McGeer, Jeff Powis, David Richardson, Makeda Semret, Vivek Shinde, Daniel Smyth, Sylvie Trottier, Louis Valiquette, Duncan Webster, Shelly A. McNeil, Philippe Lagace-Wiens, Bruce Light, Andre Poirier, Stephanie Smith, Gregory Taylor
Article
Microbiology
J. J. LeBlanc, M. ElSherif, S. Mulpuru, M. Warhuus, A. Ambrose, M. Andrew, G. Boivin, W. Bowie, A. Chit, G. Dos Santos, K. Green, S. A. Halperin, T. F. Hatchette, B. Ibarguchi, J. Johnstone, K. Katz, J. M. Langley, P. Lagace-Wiens, M. Loeb, A. Lund, D. MacKinnon-Cameron, A. McCarthy, J. E. McElhaney, A. McGeer, A. Poirier, J. Powis, D. Richardson, M. Semret, V Shinde, D. Smyth, S. Trottier, L. Valiquette, D. Webster, L. Ye, S. A. McNeil
JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Article
Infectious Diseases
Christiaan H. Righolt, Philippe Lagace-Wiens, Salaheddin M. Mahmud
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2020)
Article
Infectious Diseases
Alyssa R. Golden, Heather J. Adam, Melanie Baxter, Andrew Walkty, Philippe Lagace-Wiens, James A. Karlowsky, George G. Zhanel
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2020)
Review
Pharmacology & Pharmacy
George G. Zhanel, Jenine Esquivel, Sheryl Zelenitsky, Courtney K. Lawrence, Heather J. Adam, Alyssa Golden, Rachel Hink, Liam Berry, Frank Schweizer, Michael A. Zhanel, Denice Bay, Philippe R. S. Lagace-Wiens, Andrew J. Walkty, Joseph P. Lynch, James A. Karlowsky
Editorial Material
Medicine, General & Internal
Carl Boodman, Philippe Lagace-Wiens, Jared Bullard
CANADIAN MEDICAL ASSOCIATION JOURNAL
(2020)
Article
Public, Environmental & Occupational Health
Christiaan H. Righolt, Geng Zhang, Gregory W. Hammond, Philippe Lagace-Wiens, Salaheddin M. Mahmud
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2020)
Article
Microbiology
James A. Karlowsky, Philippe R. S. Lagace-Wiens, Nancy M. Laing, Melanie R. Baxter, Heather J. Adam, George G. Zhanel
JOURNAL OF CLINICAL MICROBIOLOGY
(2020)
Editorial Material
Medicine, General & Internal
Carl Boodman, Terence Wuerz, Philippe Lagace-Wiens
CANADIAN MEDICAL ASSOCIATION JOURNAL
(2020)
Review
Pharmacology & Pharmacy
George G. Zhanel, Christina Deng, Sheryl Zelenitsky, Courtney K. Lawrence, Heather J. Adam, Alyssa Golden, Liam Berry, Frank Schweizer, Michael A. Zhanel, Neal Irfan, Denice Bay, Philippe Lagace-Wiens, Andrew Walkty, Lionel Mandell, Joseph P. Lynch, James A. Karlowsky
Summary: Lefamulin is a novel oral and intravenous pleuromutilin developed for community-acquired bacterial pneumonia, with a unique mechanism of action and broad-spectrum antibacterial activity. It has been approved by FDA, EMA, and Health Canada for the treatment of CABP.
Article
Immunology
Carl Boodman, Catherine Smith, Ken von Kuster, Philippe Lagace-Wiens, Terence Wuerz
Summary: The ongoing epidemic of intravenous opioid and methamphetamine use in North America is associated with an increased risk of bacteremia from environmental organisms. This study reports a case of Mycobacterium mucogenicum bacteremia and soft tissue infection in a person who uses tap water to inject drugs.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Editorial Material
Medicine, General & Internal
Carl Boodman, Terence Wuerz, Philippe Lagace-Wiens
CANADIAN MEDICAL ASSOCIATION JOURNAL
(2021)
Article
Infectious Diseases
George G. Zhanel, Justin Kosar, Melanie Baxter, Rita Dhami, Sergio Borgia, Neal Irfan, Kelly S. MacDonald, Gordon Dow, Philippe Lagace-Wiens, Maxime Dube, Marco Bergevin, Carlo Tascini, Yoav Keynan, Andrew Walkty, James Karlowsky
Summary: Ceftobiprole is used in Canada as a directed therapy for treating severe infections caused by MRSA, often in combination with daptomycin or vancomycin. Treatment duration is typically over 10 days, with high rates of microbiological and clinical success and a good safety profile.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
David C. Alexander, Robert M. Taylor, Irene Martin, Heather Adam, Philippe Lagace-Wiens, Peter Pieroni, James A. Karlowsky, Paul Van Caeseele
Summary: This study compared the performance of microscopy, culture, and the Hologic Aptima Combo 2 (AC2) assay for detecting Neisseria gonorrhoeae in joint fluid specimens. The results showed that AC2 was more sensitive than culture for diagnosing gonococcal arthritis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Courtney K. Lawrence, Chris Sathianathan, Mauro Verrelli, Philippe Lagace-Wiens, Robert Ariano, Grace Badejo, Michelle L. Boyce, J. Christine Davis, Sheryl A. Zelenitsky
CANADIAN JOURNAL OF HOSPITAL PHARMACY
(2020)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
