Article
Infectious Diseases
Dusten T. Rose, Alexander Moskhos, Arya Wibisono, Kelly R. Reveles
Summary: This study evaluated the impact of using MicroScan and VITEK 2 automated systems to determine vancomycin MIC values on the treatment of MRSA bacteremia. The results showed that the MicroScan group had a higher rate of vancomycin alternative therapy use compared to the VITEK 2 group, while the VITEK 2 group had a shorter median hospital stay.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Asmaa Sadat, Radwa Reda Shata, Alshimaa M. M. Farag, Hazem Ramadan, Adel Alkhedaide, Mohamed Mohamed Soliman, Mohamed Elbadawy, Amira Abugomaa, Amal Awad
Summary: The study investigated the prevalence, antibiotic susceptibility profiles, and toxin genes of PVL-positive S. aureus in unpasteurized raw cow's milk from retail outlets in Mansoura, Egypt. The findings suggest a high level of antibiotic resistance in PVL-positive S. aureus and the presence of multiple toxin genes in the isolates.
Article
Infectious Diseases
Puthiya Purayil Preeja, Sanath H. Kumar, Veena Shetty
Summary: The study revealed the prevalence, molecular characteristics, and antibiotic resistance profiles of CA-MRSA in a tertiary care hospital in Mangalore, India. It found that CA-MRSA showed higher resistance to certain antibiotics compared to HA-MRSA, indicating the urgent need to address the emergence of multidrug-resistant CA-MRSA in healthcare settings.
Article
Microbiology
Tetsuo Yamaguchi, Itaru Nakamura, Takahiro Sato, Daisuke Ono, Ayami Sato, Shiro Sonoda, Kotaro Aoki, Yuri Miura, Shinobu Koyama, Kiyoko Tamai, Tetsuya Matsumoto, Junzo Hisatsune, Motoyuki Sugai, Yoshikazu Ishii, Kazuhiro Tatedaa
Summary: This study conducted a nationwide surveillance in Japan to investigate changes in the molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) over the past decade. The results revealed an increasing trend in the isolation of Panton-Valentine leucocidin (PVL)-positive strains, with CC8 being dominant among these strains. Additionally, a unique ST22 clone carrying both PVL and toxic shock syndrome toxin-1 genes has emerged.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Kushal Vanamala, Ketki Bhise, Hiram Sanchez, Razieh Kebriaei, Duy Luong, Samaresh Sau, Hosam Abdelhady, Michael J. Rybak, David Andes, Arun K. Iyer
Summary: This study aims to improve the efficacy of VAN treatment by identifying the folate receptor for MRSA infected tissues and developing folate decorated lipid nanoparticles containing VAN (LVAN). In comparison to conventional VAN, LVAN showed a higher bactericidal effect and a superior ability to inhibit biofilm in MRSA with an enhanced accumulation in MRSA infected thigh tissues and a reduced accumulation in kidney. The results suggested that LVAN is a promising candidate to overcome the current limitations of bacterial resistance and adverse side effects in kidneys found in VAN.
Article
Microbiology
Jan Tkadlec, Anh Vu Le, Marie Brajerova, Anna Soltesova, Jozef Marcisin, Pavel Drevinek, Marcela Krutova
Summary: The epidemiology of MRSA is characterized by the rise and fall of epidemic clones. This study aimed to gain data on the current epidemiology of MRSA in Slovakia, and it identified the presence of epidemic HA-MRSA clones and the emergence of the global epidemic USA300 CA-MRSA clone. The extensive spread of the USA300 clone in Slovakia is a significant finding.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Nabil Abdullah El Aila, Nahed Ali Al Laham, Thierry Naas
Summary: The prevalence of MRSA and PVL-positive isolates in Gaza Strip hospitals is high, reflecting a similar situation in the community. It is crucial to implement surveillance and interventions to control their spread.
Article
Pharmacology & Pharmacy
Christopher Ross, Basir Syed, Joanna Pak, Vishal Jhanji, Jason Yamaki, Ajay Sharma
Summary: The study investigated the stability of vancomycin ophthalmic drops prepared in different solutions stored at room temperature or under refrigeration for 28 days. Results showed that vancomycin ophthalmic drops prepared using PBS, BSS, and normal saline were stable up to the tested time point of 28 days, irrespective of their storage temperature.
Article
Food Science & Technology
Patricia Esteban, Sergio Redrado, Laura Comas, M. Pilar Domingo, M. Isabel Millan-Lou, Cristina Seral, Sonia Algarate, Concepcion Lopez, Antonio Rezusta, Julian Pardo, Maykel Arias, Eva M. Galvez
Summary: This study aimed to evaluate the antibiotic efficacy of Gliotoxin (GT) as monotherapy or in combination with other therapeutics against MRSA in vitro and in vivo.
Article
Infectious Diseases
Katarzyna Garbacz, Ewa Kwapisz, Lidia Piechowicz, Maria Wierzbowska
Summary: Research on bacteriophage therapy and its use in combination with antibiotics is on the rise. A study on oral Staphylococcus aureus isolates found that the majority were susceptible to phages from an international set, with different phage groups showing associations with antibiotic sensitivity in the bacteria.
Article
Engineering, Biomedical
Renee L. Tonkin, Anna Klockner, Adrian Najer, Carolina J. Simoes da Silva, Cecile Echalier, Marc S. Dionne, Andrew M. Edwards, Molly M. Stevens
Summary: This study develops a hierarchical microparticle system for bacteria-activated single- and dual-antibiotic drug delivery, effectively killing antibiotic-resistant bacteria.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Immunology
Charles Darkoh, Kadiatou Keita, Chioma Odo, Micah Oyaro, Eric L. Brown, Cesar A. Arias, Blake M. Hanson, Herbert L. DuPont
Summary: A study found that vancomycin nonsusceptible strains of Clostridioides difficile are circulating in patient populations, posing a serious challenge to treatment as vancomycin is a first-line antibiotic for CDI. Routine susceptibility testing may be necessary.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Ching-Hsi Hsiao, Eugene Yu-Chuan Kang, Lung-Kun Yeh, David H. K. Ma, Hung-Chi Chen, Kuo-Hsuan Hung, Yhu-Chering Huang
Summary: This study investigated the genotyping, antibiotic susceptibility, and clinical features of Staphylococcus aureus keratitis, with a focus on the role of Panton-Valentine leucocidin (PVL). The results showed that approximately one third of S. aureus keratitis in Taiwan was caused by community-associated MRSA. Although MRSA isolates were more resistant than MSSA, the clinical characteristics were similar between the two groups. Fluoroquinolones could be a good empirical antibiotic choice for S. aureus keratitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anaelle Fait, Dan I. Andersson, Hanne Ingmer
Summary: The loss of resistance in vancomycin-intermediate Staphylococcus aureus (VISA) strains is correlated with an increase in fitness and is attributed to adaptive mutations. Upon re-exposure to vancomycin, these revertants evolve faster and reach higher levels of resistance than vancomycin-naive cells. This suggests that previous vancomycin adaptation should be considered a risk factor when deciding on antimicrobial chemotherapy.
Review
Infectious Diseases
Marcela Krutova, Mark Wilcox, Ed Kuijper
Summary: The recommendations for the treatment of Clostridioides difficile infection (CDI) have been updated recently. The drug of choice for CDI treatment should not only possess clinical efficacy, but also exhibit optimal antimicrobial stewardship by restoring the gut microbiota quickly to minimize the risk of infection relapses. Metronidazole, the current treatment option, has low concentration in stool and reduced antimicrobial bioactivity due to interactions with fecal microbiota. Elevated minimum inhibitory concentrations of metronidazole in epidemic C. difficile ribotypes and the emergence of resistance pose potential risks for treatment failure. Oral vancomycin and fidaxomicin, on the other hand, reach high concentrations in the stool and can effectively reduce C. difficile shedding. Facilities with higher CDI incidence and/or occurrence of epidemic ribotypes should avoid using metronidazole to prevent prolonged shedding and further transmission. Fidaxomicin, with its narrow spectrum of antimicrobial activity and persistence on spores, is the preferred option to reduce recurrent CDI rates.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)