Review
Infectious Diseases
Aurelie Chauffour, Florence Morel, Florence Reibel, Stephanie Petrella, Claudine Mayer, Emmanuelle Cambau, Alexandra Aubry
Summary: This study conducted a systematic review on DNA gyrase gene mutations in M. leprae, finding mutations in 4% of clinical isolates. In response to discrepancies in FQ resistance, a consensus numbering system was proposed to properly identify mutations.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Biochemistry & Molecular Biology
Ivica Samanic, Blanka Dadic, Zeljka Sanader Marsic, Mia Dzelalija, Ana Maravic, Hrvoje Kalinic, Pavle Vrebalov Cindro, Zeljko Sundov, Marija Tonkic, Ante Tonkic, Jonatan Vukovic
Summary: This study investigated the molecular genetic mechanisms of clarithromycin and levofloxacin resistance in Helicobacter pylori infections in patients from Croatia. Point mutations in the 23S rRNA, gyrA, and gyrB genes were found to alter target sites or protein structure, leading to reduced efficacy of standard antibiotics. Molecular docking analysis revealed that resistance-related mutations resulted in decreased susceptibility to clarithromycin and levofloxacin due to significant differences in non-covalent interactions, ultimately resulting in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Suat Moi Puah, Khean Lee Goh, Heng Kang Ng, Kek Heng Chua
Summary: Resistance to clarithromycin and levofloxacin in Helicobacter pylori is a major challenge, with primary resistance rates of 14.8% and 3.3% respectively in our study. Silent mutations and polymorphisms in gyrA and gyrB genes were identified, although their role in levofloxacin resistance needs further elucidation.
Article
Microbiology
Giovanna Mendoza-Mujica, Diana Flores-Leon, Joaquim Ruiz
Summary: Amino acid changes in various genes were observed in B. bacilliformis isolates from Peru, with the main cause of ciprofloxacin resistance being substitutions in GyrA or GyrB. The bacteria show a tendency to develop mutations in GyrB outside of the typical positions seen in GyrA and ParC.
Article
Microbiology
Sheeba Santhini Manoharan-Basil, Natalia Gonzalez, Jolein Gyonne Elise Laumen, Chris Kenyon
Summary: Antimicrobial resistance in Neisseria gonorrhoeae is a significant global health concern, and horizontal gene transfer plays an important role in the development of resistance. This study identified evidence of horizontal gene transfer in fluoroquinolone resistance genes and predicted likely progenitors for these transferred genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Min Qiao, Weicong Ren, Haiping Guo, Fengmin Huo, Yuanyuan Shang, Yufeng Wang, Mengqiu Gao, Yu Pang
Summary: WFQ-228 demonstrates potent antimicrobial activity against various clinical isolates of pathogens, including FQ-resistant isolates. MFX exhibits the most potent activity against FQ-susceptible MTB, making it more effective in isolates with specific mutations conferring low-level FQ resistance. Bactericidal effect is noted more frequently in FQ-susceptible isolates than FQ-resistant isolates for MFX.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Gerald L. Murray, Erica L. Plummer, Kaveesha Bodiyabadu, Lenka A. Vodstrcil, Jose L. Huaman, Jennifer A. Danielewski, Teck Phui Chua, Dorothy A. Machalek, Suzanne Garland, Michelle Doyle, Emma L. Sweeney, David M. Whiley, Catriona S. Bradshaw
Summary: This study examined the relationship between Mycolpasma genitalium gyrA sequence variants and fluoroquinolone treatment failure. The results showed that patients with both parC S83I and gyrA sequence variations had a higher rate of moxifloxacin failure compared to those with only parC S83I variations.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Medical Laboratory Technology
Tevhide Ziver-Sarp, Pelin Yuksel-Mayda, Suat Saribas, Suleyman Demiryas, Nesrin Gareayaghi, Sevgi Ergin, Ihsan Tasci, Dogukan Ozbey, Kadir Bal, Yusuf Erzin, Seher Akkus, Hrisi Bahar-Tokman, Mehmet Demirci, Banu Tufan-Kocak, Bekir Kocazeybek
Summary: This study aimed to determine the resistance of H. pylori strains to clarithromycin and levofloxacin, and investigate the mutations responsible for levofloxacin resistance. The findings showed that mutations in the gyrA gene had an enhancing effect on levofloxacin resistance, while dual resistance developed with increased rates of clarithromycin and levofloxacin resistance.
CLINICAL LABORATORY
(2021)
Article
Infectious Diseases
Jing Bi, Qinglong Guo, Xiangdong Fu, Juan Liang, Lidong Zeng, Min Ou, Juanjuan Zhang, Zhaoqin Wang, Yicheng Sun, Lei Liu, Guoliang Zhang
Summary: The study identified mutations in the gyrA and gyrB genes as the main mechanisms of GAT resistance. These findings provide new insights into molecular diagnosis of GAT resistance in the clinical setting.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Raj Kishor Sharma, Usha Kumari, Namrata Kumari, Rakesh Kumar
Summary: This study aims to characterize genetic mutations in MDR isolates of Mycobacterium tuberculosis in the Eastern Indian population, and identify drug resistance-related gene mutations. Monitoring predominant mutations in the MDR-TB patient population is essential for future therapeutic regimes.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Infectious Diseases
Sacha J. Pidot, Jessica L. Porter, Troy Lister, Timothy P. Stinear
Summary: Nontuberculosis mycobacterial (NTM) infections are on the rise globally, with limited treatment options available. However, the development of new antimycobacterial drugs shows promise in addressing this issue. The novel antibiotic SPR719 has shown activity against NTM species, with a MIC range of 0.125-4 μg/ml, comparable to commonly used antimycobacterial antibiotics such as rifampicin and clarithromycin. Further evaluation of SPR720 for NTM infection treatment is warranted.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Jianfeng Wang, Chu Yang, Chao Zhang, Xiaoyan Mao, An Lizhe
Summary: In this study, the genome of Clostridium difficile was studied using metagenomic technology, revealing its gene sequence information and potential toxicological genes. Through gene function analysis and pathogenicity analysis, the metabolism activity, catalytic activity, carbohydrate-active enzyme genes and other aspects of the bacterium were understood, and an assessment was made on its virulence genes.
Review
Microbiology
Marta Mattana, Riccardo Tomasello, Claudia Cammarata, Paola Di Carlo, Teresa Fasciana, Giulio Giordano, Alessandro Lucchesi, Sergio Siragusa, Mariasanta Napolitano
Summary: This review analyzed the correlation between coagulation complications related to C. difficile infection and inflammasome activation, particularly the pyrin-dependent one. The evidence suggests that inflammasome activation increases the risk of venous thromboembolism, and inhibition of molecules involved in coagulation activation could block this process. It may be possible to reduce complications and mortality associated with C. difficile infection by reducing inflammatory activity and preventing thromboembolic complications.
Review
Biotechnology & Applied Microbiology
Deirdre A. Collins, Thomas Riley
Summary: Clostridium difficile infection (CDI) is a urgent threat to patients worldwide, and recurrent CDI remains a high risk due to disruption of the gut microbiota. Traditional therapy with vancomycin and metronidazole carries a high risk of recurrence, leading to the development of novel narrow spectrum antimicrobial agents like fidaxomicin and ridinilazole. Ridinilazole shows promising results in vitro and in animal models, and phase I and II clinical trials have demonstrated its efficacy and superiority over vancomycin in reducing recurrence of CDI. Phase III trials are currently ongoing to further evaluate its potential in reducing recurrent CDI and its impact on healthcare systems.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Ibrahim A. Al-Zahrani
Summary: Clostridioides difficile (C. difficile) infection is a global threat to many healthcare settings. Excessive use of antimicrobials, lack of optimal antibiotic policies, and suboptimal infection control practices have fueled the development of this health issue. Prudent use of antimicrobials and simple infection control measures can significantly reduce infection rates.
SAUDI MEDICAL JOURNAL
(2023)
Article
Immunology
Carol Strahm, Marco Seneghini, Sabine Gusewell, Thomas Egger, Onicio Leal-Neto, Angela Brucher, Eva Lemmenmeier, Dorette Meier Kleeb, J. Carsten Moeller, Philip Rieder, Markus Ruetti, Remus Rutz, Hans-Ruedi Schmid, Reto Stocker, Danielle Vuichard-Gysin, Benedikt Wiggli, Ulrike Besold, Stefan P. Kuster, Allison McGeer, Lorenz Risch, Andree Friedl, Matthias Schlegel, Dagmar Schmid, Pietro Vernazza, Christian R. Kahlert, Philipp Kohler
Summary: In a study among healthcare workers, participants with a positive nasopharyngeal swab for SARS-CoV-2 were more likely to experience long COVID symptoms, while seropositive participants without a positive swab had only mild effects. Additionally, baseline physical activity was negatively associated with neurocognitive impairment and fatigue symptoms.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Christopher E. Kandel, Andra Banete, Maureen Taylor, Andrea Llanes, Janine McCready, Gloria Crowl, Matthew Young, Angel X. Li, Emily Chien, Winfield Yim, Lily Yip, Robert Kozak, Allison J. McGeer, Samira Mubareka, Jeff E. Powis
Summary: There was no difference in viral shedding between outpatients with coronavirus disease 2019 (COVID-19) due to the severe acute respiratory coronavirus virus 2 (SARS-CoV-2) delta (delta) variant who received 2 vaccine doses at 7 days after symptom onset and those who did not receive the vaccine, with SARS-CoV-2 cultured from 2 (7%) of 28 and 1 (4%) of 26 outpatients, respectively.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Letter
Public, Environmental & Occupational Health
Ahmed Vanker, Lara de Waal, Allison McGeer, Matthew Morgan
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Letter
Immunology
Matthew P. Muller, Sharmistha Mishra, Allison McGeer, Samir Patel, Jonathan Gubbay, Maan Hasso, Adrienne K. Chan, Robert Kozak, Jerome A. Leis, Darrell S. Tan
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Julie A. Bettinger, Michael A. Irvine, Hennady P. Shulha, Louis Valiquette, Matthew P. Muller, Otto G. Vanderkooi, James D. Kellner, Karina A. Top, Manish Sadarangani, Allison McGeer, Jennifer E. Isenor, Kimberly Marty, Phyumar Soe, Gaston De Serres
Summary: This study examined the short-term safety of COVID-19 vaccines in adults with a previous history of SARS-CoV-2 infection. It found that adults with moderate or severe previous infection were more likely to experience health events after each vaccine dose, while the risk associated with previous infection was attenuated after subsequent doses.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Genetics & Heredity
Selina Casalino, Erika Frangione, Monica Chung, Georgia MacDonald, Sunakshi Chowdhary, Chloe Mighton, Hanna Faghfoury, Yvonne Bombard, Lisa Strug, Trevor J. Pugh, Jared Simpson, Saranya Arnoldo, Navneet Aujla, Erin Bearss, Alexandra Binnie, Bjug Borgundvaag, Howard Chertkow, Marc Clausen, Marc Dagher, Luke Devine, David Di Iorio, Steven Marc Friedman, Chun Yiu Jordan Fung, Anne-Claude Gingras, Lee W. Goneau, Deepanjali Kaushik, Zeeshan Khan, Elisa Lapadula, Tiffany Lu, Tony Mazzulli, Allison McGeer, Shelley L. McLeod, Gregory Morgan, David Richardson, Harpreet Singh, Seth Stern, Ahmed Taher, Iris Wong, Natasha Zarei, Elena Greenfeld, Limin Hao, Matthew Lebo, William Lane, Abdul Noor, Jennifer Taher, Jordan Lerner-Ellis
Summary: Rapid advancements in genome sequencing technology have improved our understanding of the relationship between genes and human disease. This study collected blood samples from COVID-19 patients and performed genome sequencing to analyze the data. The researchers developed a comprehensive report that includes information on disease risks, genetic variants, and ancestry. They emphasize the importance of genetic counseling and offer counseling and referrals for clinically significant findings.
Article
Immunology
Jacqui van Warmerdam, Aaron Campigotto, Ari Bitnun, Georgina MacDougall, Melanie Kirby-Allen, Blake Papsin, Allison McGeer, Upton Allen, Shaun K. Morris
Summary: Despite the availability of pneumococcal vaccines, children with high-risk conditions are still susceptible to invasive pneumococcal disease. Insufficient vaccination is the main factor contributing to the occurrence of the disease.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Correction
Microbiology
Bradley Pickering, Oliver Lung, Finlay Maguire, Peter Kruczkiewicz, Jonathon D. Kotwa, Tore Buchanan, Marianne Gagnier, Jennifer L. Guthrie, Claire M. Jardine, Alex Marchand-Austin, Ariane Masse, Heather McClinchey, Kuganya Nirmalarajah, Patryk Aftanas, Juliette Blais-Savoie, Hsien-Yao Chee, Emily Chien, Winfield Yim, Andra Banete, Bryan D. Griffin, Lily Yip, Melissa Goolia, Matthew Suderman, Mathieu Pinette, Greg Smith, Daniel Sullivan, Josip Rudar, Oksana Vernygora, Elizabeth Adey, Michelle Nebroski, Guillaume Goyette, Andres Finzi, Genevieve Laroche, Ardeshir Ariana, Brett Vahkal, Marceline Cote, Allison J. McGeer, Larissa Nituch, Samira Mubareka, Jeff Bowman
NATURE MICROBIOLOGY
(2023)
Article
Immunology
Hannah Chung, Michael A. Campitelli, Sarah A. Buchan, Aaron Campigotto, Branson Chen, Natasha S. Crowcroft, Vinita Dubey, Jonathan B. Gubbay, Timothy Karnauchow, Kevin Katz, Allison J. McGeer, J. Dayre McNally, Samira Mubareka, Michelle Murti, David C. Richardson, Laura C. Rosella, Kevin L. Schwartz, Marek Smieja, George Zahariadis, Jeffrey C. Kwong
Summary: The effectiveness of influenza vaccine against laboratory-confirmed influenza was similar between statin users and nonusers. However, statin users had a higher risk of influenza infection compared to nonusers, regardless of vaccination status. This study suggests that statin use may affect the effectiveness of influenza vaccine and increase the risk of influenza infection.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Dylan C. Kain, Sandra Isabel, Mariana Abdulnoor, Karel Boissinot, Richard De Borja, Amanda Filkin, Bernard Lam, Jason Li, Ilinca Lungu, Liz McCreight, Allison McGeer, Tony Mazzulli, Aimee Paterson, Philip Zuzarte, Felicia Vincelli, Cassandra Bergwerff, Ramzi Fattouh, Jared T. Simpson, Jennie Johnstone
Summary: During the COVID-19 pandemic, a severe SARS-CoV-2 outbreak occurred in a hospital in Toronto, Canada, from November 2020 to January 2021. The outbreak involved 8 patients and 10 staff and resulted in 3 patient deaths. Investigation revealed that patients cared for in geriatric chairs at the nursing station were at high risk for both acquiring and transmitting SARS-CoV-2. Therefore, it is important to consider the risk of informal patient care settings and prioritize room-based care during high-risk periods or outbreaks.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Economics
Ryan O'Reilly, Hong Lu, Jeffrey C. C. Kwong, Allison McGeer, Teresa To, Beate Sander
Summary: The present study aimed to determine the short- and long-term healthcare costs associated with community-acquired pneumonia (CAP) from the healthcare payer perspective in Ontario, Canada. Through a retrospective population-based matched cohort study, it was found that CAP is associated with significantly increased acute and long-term healthcare costs compared to unexposed subjects. This study highlights the burden of CAP in both the inpatient and outpatient setting, and will inform strategic healthcare planning for future interventions and healthcare programs.
JOURNAL OF MEDICAL ECONOMICS
(2023)
Article
Infectious Diseases
Kuganya Nirmalarajah, Winfield Yim, Patryk Aftanas, Angel X. Li, Altynay Shigayeva, Lily Yip, Zoe Zhong, Allison J. Mcgeer, Finlay Maguire, Samira Mubareka, Robert Kozak
Summary: This study investigated the effects of Remdesivir (RDV) treatment on intra-host SARS-CoV-2 diversity and low-frequency mutations in moderately ill hospitalized COVID-19 patients. The results showed minimal intra-host variability and few low-frequency variants in patients receiving short courses of RDV, suggesting little selective pressure.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2023)
Article
Geriatrics & Gerontology
Pamela Leece, Michael Whelan, Andrew P. Costa, Nick Daneman, Jennie Johnstone, Allison McGeer, Paula Rochon, Kevin L. Schwartz, Kevin A. Brown
Summary: Studies conducted before the COVID-19 pandemic have found that crowding in nursing homes is associated with higher incidence of respiratory infections and mortality. This association is consistent across various respiratory pathogens. Decreasing crowding is important for resident wellbeing and reducing the transmission of respiratory pathogens.
LANCET HEALTHY LONGEVITY
(2023)
Letter
Immunology
Sharon Sukhdeo, Matthew Muller, Allison McGeer, Jerome A. Leis, Adrienne Chan, Jonathan B. Gubbay, Samir Patel, Saman Khan, Stephen Perusini, Xinliu Angel Li, Robert Kozak, Sharmistha Mishra, Darrell H. S. Tan, Christopher Kandel
OPEN FORUM INFECTIOUS DISEASES
(2023)
Meeting Abstract
Medical Laboratory Technology
Gregory Morgan, Marc Clausen, Selina Casalino, Chloe Mighton, Sunakshi Chowdhary, Erika Frangione, Monica Chung, Chun Yiu Jordan Fung, Georgia MacDonald, Elisa Lapadula, Saranya K. Arnoldo, Erin Bearss, Alexandra Binnie, Bjug Borgundvaag, Howard Chertkow, Marc Dagher, Luke Devine, Hanna Faghfoury, Steven M. Friedman, Anne-Claude Gingras, Lee W. Goneau, Zeeshan Khan, Tony Mazzulli, Allison McGeer, Shelley L. McLeod, Konika Nirmalanathan, Trevor J. Pugh, David C. Richardson, Jared Simpson, Seth Stern, Lisa Strug, Ahmed Taher, Iris Wong, Natasha Zarei, Yvonne Bombard, Jordan Lerner-Ellis, Jennifer Taher
CLINICAL BIOCHEMISTRY
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)