Article
Immunology
Yodeline Guillaume, Meti Debela, Damien Slater, Kenia Vissieres, Ralph Ternier, Molly F. Franke, Jason B. Harris, Louise C. Ivers
Summary: We report that stool culture may not be sufficiently robust in detecting Vibrio cholerae in Haiti during the waning period of the initial outbreak in 2018-2019, with a sensitivity of 33.3% and specificity of 97.4%.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Bolutife Fakoya, Karthik Hullahalli, Daniel H. F. Rubin, Deborah R. Leitner, Roma Chilengi, David A. Sack, Matthew K. Waldor
Summary: This study created next-generation cholera challenge strains and demonstrated their accuracy and safety in measuring immunity and vaccine effectiveness.
Article
Microbiology
Yong Yan, Li Zhan, Guoying Zhu, Junyan Zhang, Ping Li, Lixia Chen, Peiyan He, Jianyong Luo, Zhongwen Chen
Summary: A novel multiplex assay for rapid detection of Vibrio cholerae serogroup and cholera toxin toxigenicity was developed. The assay showed good diagnostic sensitivities and is suitable for rapid identification and screening of large specimens.
Review
Medicine, General & Internal
David A. Montero, Roberto M. Vidal, Juliana Velasco, Sergio George, Yalda Lucero, Leonardo A. Gomez, Leandro J. Carreno, Richard Garcia-Betancourt, Miguel O'Ryan
Summary: This review provides an overview of the causative agent of cholera, Vibrio cholerae, including its evolution, pathogenesis, and the immune response against it. It highlights the adaptability of V. cholerae and its virulence factors, as well as the importance of vaccination in controlling cholera outbreaks. The review also discusses current and future vaccine developments.
FRONTIERS IN MEDICINE
(2023)
Article
Microbiology
Kaoru Ochi, Tamaki Mizuno, Prosenjit Samanta, Asish K. Mukhopadhyay, Shin-ichi Miyoshi, Daisuke Imamura
Summary: Cholera, caused by pathogenic strains of V. cholerae generated by lysogenization of the filamentous cholera toxin phage CTX Phi, has recently shown alterations in the CTX Phi prophage array, resulting in an inability to replicate the genome. This change suggests a significant shift in the dissemination pattern of the current cholera pandemic.
Article
Microbiology
Haeun Kim, Brianne J. Burkinshaw, Linh G. Lam, Kevin Manera, Tao G. Dong
Summary: This study identified two compounds that effectively target the growth of V. cholerae, potentially providing a new approach to control cholera infections.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Pan Lu, Jialiang Chen, Zhenpeng Li, Zhe Li, Jingyun Zhang, Biao Kan, Bo Pang
Summary: In this study, a CARID system was developed for rapid detection of toxigenic V. cholerae serogroups. The system showed rapid, sensitive, economically efficient, and portable features, and was suitable for detecting complex samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Dustin A. Farr, Dhrubajyoti Nag, Jeffrey H. Withey
Summary: The study reveals that zebrafish can serve as a natural host model for Vibrio cholerae, exhibiting similar diarrheal symptoms as humans. By assessing various immunological markers, it was found that infection with V. cholerae in zebrafish leads to increased gene expression of T-bet and GATA3, as well as elevated levels of mucosal antibodies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Paul Breen, Andrew D. Winters, Kevin R. Theis, Jeffrey H. Withey
Summary: Zebrafish provide an attractive model for studying host-microbe interactions, with the core intestinal microbiome primarily consisting of Proteobacteria. Vibrio cholerae can significantly alter the zebrafish intestinal microbiome, with different strains inducing varying changes. Specific microbes targeted by V. cholerae for colonization in zebrafish hosts depend on the genotype of the V. cholerae strain.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Tania Nasreen, Nora A. S. Hussain, Jia Yee Ho, Vanessa Zhi Jie Aw, Munirul Alam, Stephanie K. Yanow, Yann F. Boucher
Summary: This article describes a method for rapid detection of Vibrio cholerae in water samples without the need for DNA extraction. It is useful for field research in low-resource settings and can support continuous monitoring in cholera-endemic areas.
Article
Food Science & Technology
Donghyun Lee, Hunseok Choi, Seonghyeon Son, Jonghyun Bae, Jayun Joo, Dong Wook Kim, Eun Jin Kim
Summary: The expression of virulence genes in Vibrio cholerae is regulated by the ToxR regulon, which is triggered by environmental stimuli during infection in the human small intestine. This study investigates the induction of virulence gene expression under laboratory culture conditions through two point mutations in the toxT gene. The findings suggest that the regulation of virulence gene expression may be more complex than previously thought, involving factors beyond the ToxR regulon.
Article
Environmental Sciences
Sadok Khouadja, Ana Roque, Mar Gonzalez, Dolors Furones
Summary: In this study, we investigated the presence of Vibrio cholerae virulence genes in Vibrio alginolyticus isolates and found that some of the V. alginolyticus strains might have derived their virulence genes from V. cholerae, making them a possible reservoir of V. cholerae virulence genes.
JOURNAL OF WATER AND HEALTH
(2022)
Review
Chemistry, Medicinal
Denise Chac, Chelsea N. Dunmire, Jasneet Singh, Ana A. Weil
Summary: This review discusses the host and environmental factors that impact susceptibility to V. cholerae infection and disease severity, emphasizing the ingestion of drinking water contaminated with fecal matter as the primary route of cholera transmission.
ACS INFECTIOUS DISEASES
(2021)
Article
Plant Sciences
Shruthi Vasanth, Regina Sumitha Mohanraj, Jharna Mandal
Summary: Centella asiatica extract inhibited the production of cholera toxin in Vibrio cholerae and reduced the transcription of ctxA gene expression.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Genetics & Heredity
Matthew J. Dorman, Nicholas R. Thomson
Summary: Between 1965 and 1968, cholera outbreaks in Sudan and former Czechoslovakia caused public health concern. These events were caused by a clone of toxigenic V. cholerae O37, suggesting its global distribution beyond Europe and North Africa. Understanding the biology of non-O1 strains of V. cholerae is crucial in comprehending the global significance of cholera. This article consolidates epidemiological, molecular, and genomic descriptions of the bacteria responsible for these outbreaks.
MICROBIAL GENOMICS
(2023)
Article
Biochemical Research Methods
Warawan Wongboot, Kazuhisa Okada, Siriporn Chantaroj, Watcharaporn Kamjumphol, Shigeyuki Hamada
JOURNAL OF MICROBIOLOGICAL METHODS
(2018)
Article
Multidisciplinary Sciences
Kazuhisa Okada, Warawan Wongboot, Siriporn Chantaroj, Wirongrong Natakuathung, Amonrattana Roobthaisong, Watcharaporn Kamjumphol, Fumito Maruyama, Taichiro Takemura, Ichiro Nakagawa, Makoto Ohnishi, Shigeyuki Hamada
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Kazuhisa Okada, Mathukorn Na-Ubol, Wirongrong Natakuathung, Amonrattana Roobthaisong, Fumito Maruyama, Ichiro Nakagawa, Siriporn Chantaroj, Shigeyuki Hamada
Article
Multidisciplinary Sciences
Yo Sugawara, Yukihiro Akeda, Noriko Sakamoto, Dan Takeuchi, Daisuke Motooka, Shota Nakamura, Hideharu Hagiya, Norihisa Yamamoto, Isao Nishi, Hisao Yoshida, Kazuhisa Okada, Khwar Nyo Zin, Mya Mya Aye, Kazunori Tonomo, Shigeyuki Hamada
Editorial Material
Infectious Diseases
Watcharaporn Kamjumphol, Warawan Wongboot, Namfon Suebwongsa, Pipat Kluabwang, Siriporn Chantaroj, Kazuhisa Okada
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2019)
Article
Public, Environmental & Occupational Health
Norihisa Yamamoto, Shigeto Hamaguchi, Yukihiro Akeda, Pitak Santanirand, Narong Chaihongsa, Suntariya Sirichot, Suwichak Chiaranaicharoen, Hideharu Hagiya, Kouji Yamamoto, Anusak Kerdsin, Kazuhisa Okada, Hisao Yoshida, Shigeyuki Hamada, Kazunori Oishi, Kumthorn Malathum, Kazunori Tomono
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2019)
Article
Multidisciplinary Sciences
Kazuhisa Okada, Warawan Wongboot, Watcharaporn Kamjumphol, Namfon Suebwongsa, Piyada Wangroongsarb, Pipat Kluabwang, Nuttagarn Chuenchom, Witaya Swaddiwudhipong, Thanee Wongchai, Weerawat Manosuthi, Norrathep Assawapatchara, Patchanee Khum-on, Patpong Udompat, Chareeya Thanee, Suwatthiya Kitsaran, Lakkana Jirapong, Charoen Jaiwong, Supalert Nedsuwan, Chotipong Siripipattanamongkol, Pilailuk Akkapaiboon Okada, Siriporn Chantaroj, Sho Komukai, Shigeyuki Hamada
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Masatomo Morita, Kazuhisa Okada, Tetsu Yamashiro, Tsuyoshi Sekizuka, Amonrattana Roobthaisong, Warawan Wongboot, Siriporn Chantaroj, Nguyen Dong Tu, Phonepadith Xangsayarath, Noikaseumsy Sithivong, Khambai Noilath, Arounnapha Vongdouangchanh, Makoto Kuroda, Shigeyuki Hamada, Hidemasa Izumiya, Makoto Ohnishi
OPEN FORUM INFECTIOUS DISEASES
(2020)
Article
Environmental Sciences
Josh M. Colston, Benjamin F. Zaitchik, Hamada S. Badr, Eleanor Burnett, Syed Asad Ali, Ajit Rayamajhi, Syed M. Satter, Daniel Eibach, Ralf Krumkamp, Jurgen May, Roma Chilengi, Leigh M. Howard, Samba O. Sow, M. Jahangir Hossain, Debasish Saha, M. Imran Nisar, Anita K. M. Zaidi, Suman Kanungo, Inacio Mandomando, Abu S. G. Faruque, Karen L. Kotloff, Myron M. Levine, Robert F. Breiman, Richard Omore, Nicola Page, James A. Platts-Mills, Ulla Ashorn, Yue-Mei Fan, Prakash Sunder Shrestha, Tahmeed Ahmed, Estomih Mduma, Pablo Penatero Yori, Zulfiqar Bhutta, Pascal Bessong, Maribel P. Olortegui, Aldo A. M. Lima, Gagandeep Kang, Jean Humphrey, Andrew J. Prendergast, Robert Ntozini, Kazuhisa Okada, Warawan Wongboot, James Gaensbauer, Mario T. Melgar, Tuula Pelkonen, Cesar Mavacala Freitas, Margaret N. Kosek
Summary: Diarrheal disease, a major health problem for children, is influenced by climate factors such as temperature, humidity, and soil moisture. This study analyzed data from multiple countries and found that different enteropathogens have different responses to climate variables. Temperature, soil moisture, and humidity are particularly influential parameters, and climate change may lead to shifts in the relative burden of diarrhea-causing agents.
Article
Immunology
Kazuhisa Okada, Amonrattana Roobthaisong, Suthida Muangnoicharoen Hearn, Pilailuk Akkapaiboon Okada, Pawinee Doung-Ngern, Warawan Wongboot, Atchareeya Nakkarach, Masatomo Morita, Toshio Kodama, Tetsuya Iida
Summary: We report the first case of infection due to Vibrio parahaemolyticus O10:K4 isolated from a hospitalized patient with acute diarrhea in Thailand. The whole genome of the O10:K4 strain was sequenced and compared to other strains, confirming the spread of the O10:K4 strain to Southeast Asia.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Microbiology
Kazuhisa Okada, Amonrattana Roobthaisong, Shigeyuki Hamada
Summary: Understanding the survival strategies of pandemic cholera pathogens in aquatic environments is important for preventing their dissemination. Long-term cultivation of Vibrio cholerae under nutrient-limited conditions causes mutations in flagella-related genes, inhibiting motility. These mutations also reduce the transition to the viable but nonculturable state of the organisms. Longer cultivation leads to further mutations in metabolism-related genes and the loss of virulence factors and large DNA regions.
Article
Public, Environmental & Occupational Health
Hamada S. Badr, Josh M. Colston, Nhat-Lan H. Nguyen, Yen Ting Chen, Eleanor Burnett, Syed Asad Ali, Ajit Rayamajhi, Syed M. Satter, Nguyen Van Trang, Daniel Eibach, Ralf Krumkamp, Juergen May, Ayola Akim Adegnika, Gedeon Prince Manouana, Peter Gottfried Kremsner, Roma Chilengi, Luiza Hatyoka, Amanda K. Debes, Jerome Ateudjieu, Abu S. G. Faruque, M. Jahangir Hossain, Suman Kanungo, Karen L. Kotloff, Inacio Mandomando, M. Imran Nisar, Richard Omore, Samba O. Sow, Anita K. M. Zaidi, Nathalie Lambrecht, Bright Adu, Nicola Page, James A. Platts-Mills, Cesar Mavacala Freitas, Tuula Pelkonen, Per Ashorn, Kenneth Maleta, Tahmeed Ahmed, Pascal Bessong, Zulfiqar A. Bhutta, Carl Mason, Estomih Mduma, Maribel P. Olortegui, Pablo Penataro Yori, Aldo A. M. Lima, Gagandeep Kang, Jean Humphrey, Robert Ntozini, Andrew J. Prendergast, Kazuhisa Okada, Warawan Wongboot, Nina Langeland, Sabrina J. Moyo, James Gaensbauer, Mario Melgar, Matthew Freeman, Anna N. Chard, Vonethalom Thongpaseuth, Eric Houpt, Benjamin F. Zaitchik, Margaret N. Kosek
Summary: This study aimed to model the spatiotemporal variation in paediatric Shigella infection and predict its prevalence across low-income and middle-income countries. The findings revealed the association between Shigella and climatological factors, with sub-Saharan Africa being a high-risk region for transmission. These findings can guide prioritization of populations for future vaccine trials and campaigns.
LANCET GLOBAL HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Amonrattana Roobthaisong, Kazuhisa Okada, Nilar Htun, Wah Wah Aung, Warawan Wongboot, Watcharaporn Kamjumphol, Aye Aye Han, Yi Yi, Shigeyuki Hamada
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2017)
Letter
Immunology
Kazuhisa Okada, Wirongrong Natakuathung, Mathukorn Na-Ubol, Amonrattana Roobthaisong, Warawan Wongboot, Fumito Maruyama, Ichiro Nakagawa, Siriporn Chantaroj, Shigeyuki Hamada
EMERGING INFECTIOUS DISEASES
(2015)
Letter
Immunology
Wah Wah Aung, Kazuhisa Okada, Mathukorn Na-Ubol, Wirongrong Natakuathung, Toe Sandar, Nan Aye Thidar Oo, Mya Mya Aye, Shigeyuki Hamada
EMERGING INFECTIOUS DISEASES
(2015)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
